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Research Article

Agricultural Science Digest, volume 35 issue 3 (september 2015) : 229-232

Production of indole-3-acetic acid by berseem (Trifolium alexandrinum L.) rhizobia isolated from Haryana, India

Varsha Garg, Kamlesh Kukreja, Rajesh Gera, Ankit Singla
1Department of Microbiology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India.
Cite article:- Garg Varsha, Kukreja Kamlesh, Gera Rajesh, Singla Ankit (2024). Production of indole-3-acetic acid by berseem (Trifolium alexandrinum L.) rhizobia isolated from Haryana, India. Agricultural Science Digest. 35(3): 229-232. doi: 10.5958/0976-0547.2015.00053.1.

ABSTRACT

A total of 40 Rhizobium strains were isolated from the root nodules of Berseem (Trifolium alexandrinum L.) crop which was collected from 17 different villages of three districts (Hisar, Sirsa and Karnal) of Haryana state. Morphologically, biochemically and finally by plant infectivity test, they were recognized as rhizobia. All the isolated strains were tested for the production of indole-3-acetic acid (a phytohormone having important physiological implications). IAA production was checked quantitatively in YEM (yeast extract mannitol) medium supplemented with 0.1 mgml-1 L-tryptophan. All the 40 Rhizobium strains were found to be IAA producers after 72 h; however their production amount varied significantly. Rhizobium strain BH14 was found to elaborate maximum amount of IAA (176 µgml-1) followed by BH13 (147.6 µgml-1) and BH45 (147.4 µgml-1). All the three superior IAA producers belonged to district Hisar. These results confirm the potential of some strains of R. leguminosarum bv trifolii as biofertilizers.

REFERENCES

  1. Ahmad, F., Ahmad, I. and Khan, M.S. (2005). Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turk. J. Biol. 29:29-34.
  2. Asghar, H.N., Zahir, Z.A. and Arshad, M. (2004). Screening rhizobacteria for improving the growth, yield and oil content of canola (Brassica nappus L.). Aust. J. Agric. Res. 55:187-194.
  3. Basu, P.S. and Ghosh, A.C. (2001). Production of Indole Acetic Acid in cultures by a Rhizobium species from the root nodules of a monocotyledonous tree, Roystonea regia. Acta. Biotechnol. 21:65-72.
  4. Bose, A., Shah, D. and Keharia, H. (2013). Production of indole-3-acetic-acid (IAA) by the white rot fungus Pleurotus ostreatus under submerged condition of Jatropha seedcake Mycology: An Int. J. Fungal Biol. 4:103-111.
  5. Dasgupta, D., Sengupta, C. and Paul, G. (2015). Screening and Identification of Best Three Phosphate Solubilizing and IAA Producing PGPR Inhabiting the Rhizosphere of Sesbania Bispinosa. Int. J. Innov. Res. Sci. Eng. Technol. 4:3968-3979.
  6. Datta, C. and Basu, P.S. (2000). Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub, Cajanus cajan. Microbiol. Res. 155:123-127.
  7. Davies P. J. (2010). The plant hormones: Their nature, occurrence, and functions. In: Plant Hormones. Editors: Davis P J. Publisher: Kluwer Academic Publishers. Netherlands. 1-15.
  8. Deshwal V.K., Dubey, R.C. and Maheshwari, D.K. (2003). Isolation of plant growth promoting strains of Bradyrhizobium (Arachis sp.) with biocontrol potential against Macrophomina causing charcoal root of peanut. Curr. Sci. 84:443-448.
  9. Erum, S. and Bano, A. (2008). Variation in Phytohormone Production in Rhizobium Strains at Different Altitudes of Nothern Areas of Pakistan. Int. J. Agr. Biol. 10:536-40.
  10. Ghosh, A.C. and Basu, P.S. (2002). Growth behaviour and bioproduction of indole acetic acid by a Rhizobium species isolated from root nodules of a leguminous tree Dalbergia lanceolarea. Indian J. Exp. Biol. 40:796-801.
  11. Ghosh, S. and Basu, P.S. (2006). Production and metabolism of indole acetic acid in roots and root nodules of Phaseolus mungo. Microbiol. Res. 161:362-366.
  12. Ghosh, S., Sengupta, C., Maiti, T.K. and Basu, P.S. (2008). Production of 3-indolyl acetic acid in root nodules and culture by a Rhizobium species isolated from root nodules of the leguminous pulse Phaseolous mungo. Folia Microbiol. 53:351-355.
  13. Gordon, S.A. and Weber, R.P. (1951). Colorimetric estimation of indole acetic acid. Plant Physiol. 26:192-195.
  14. Hussain, A., Shah, S.T., Rahman, H., Irshad, M. and Iqbal, A. (2015). Effect of IAA on in vitro growth and colonization of Nostoc in plant roots: Front. Plant Sci. 6:1-9.
  15. Khalid, A., Tahir, S., Arshad, M. and Zahir, Z.A. (2004). Relative efficiency of rhizobacteria for auxin biosynthesis in rhizosphere and non-rhizosphere soils. Aus. J. Soil Res. 42:921-926.
  16. Kumar, R. P. and Ram, M.R. (2012). Production of indole acetic acid by Rhizobium isolates from Vigna trilobata (L) Verdc. Afr. J. Microbiol. Res. 6:5536-5541.
  17. Laghari, H.H., Channa, A.D., Solangi, A.A. and Soomro, S.A. (2000). Comparative digestibility of different cuts of berseem (Trifolium alexandrinum L.) in sheep. Pak. J. Biol. Sci. 3:1938-1939.
  18. Mandal, S.M., Mondal, K.C., Dey, S. and Pati, B.R. (2007). Optimization of cultural and nutritional conditions for indole-3-acetic acid (IAA) production by a Rhizobium sp. isolated from root nodules of Vigna mungo (L.) Hepper. Res. J. Microbiol. 2:239-246.
  19. Mohite, B. (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J. Soil Sci. Plant Nutr. 13:638-649.
  20. Patten, C.L. and Glick, B.R. (1996). Bacterial biosynthesis of indole-3-acetic acid. Can. J. Microbiol. 42:207-20.
  21. Sahasrabudhe, M.M. (2011). Screening of rhizobia for indole acetic acid production. Ann. Biol. Res. 2:460-468.
  22. Sasirekha, B., Shivakumar, S. and Sullia, S.B. (2012). Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion. J. Soil Sci. Plant Nutr. 12:863-873.
  23. Sergeeva, E., Liaaimer, A. and Bergman, B. (2002). Evidence for production of the phytohormone indole -3- acetic acid by cyanobacteria. Planta. 215:229-238.
  24. Spaepen, S. and Vanderleyden, J. (2011). Auxin and Plant-Microbe Interactions. Cold Spring Harb. Perspect. Biol. 3:1-13.
  25. Sridevi, M. and Mallaiah, K.V. (2007). Bioproduction of indole acetic acid by Rhizobium strains isolated from root nodules of green manure crop, Sesbania sesban (L.) Merr. Iranian J. Biotech. 5:178-182.
  26. Sridevi, M. and Mallaiah, K.V. (2008). Production of indole-3-acetic acid by Rhizobium isolates from Sesbania species. Plant Sci. Res. 1:13-16.
  27. Varalakshmi, P. and Malliga, P. (2012). Evidence for production of Indole-3-acetic acid from a fresh water cyanobacteria (Oscillatoria annae) on the growth of H. annus. Int. J. Sci. Res. Publications. 2:1-15.
  28. Vargas, L.K., Lisboa, B.B., Schlindwein, G., Granada,C.E., Giongo, A., Beneduzi, A. and Passaglia, L.M.P. (2009). Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state. Rev. Bras. Cienc. Solo. 33:1227-1235.
  29. Verma, P., Chandra, A., Roy, A.K., Malaviya, D.R., Kaushal, P., Pandey, D. and Bhatia, S. (2015). Development, characterization and cross-species transferability of genomic SSR markers in berseem (Trifolium alexandrinum L.), an important multi-cut annual forage legume. Mol. Breeding. 35:1-14.
  30. Vijila, K. (2000). Estimation of IAA production in nitrogen fixing microorganisms. Practical manual-microbial interaction in soil. Tamil Nadu Agricultural University, Coimbatore, pp. 38-39.
  31. Vincent, J.M. (1970). A Manual for the Practical Study of the Root Nodule Bacteria. Blackwell Scientific Publications, Oxford. pp.7-9.
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