Agricultural Science Digest

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Agricultural Science Digest, volume 34 issue 4 (december 2014) : 281 - 284

SHELF LIFE STUDY OF FORMULATIONS OF FUNGAL AND BACTERIAL ANTAGONISTS AS BIOINOCULANTS

Meenu Gupta*, N.P. Dohroo
1Department of Vegetable Science, Dr. Y. S. Parmar University of Horticulture & Forestry, Nauni, Solan-173 230, India
Cite article:- Gupta* Meenu, Dohroo N.P. (2024). SHELF LIFE STUDY OF FORMULATIONS OF FUNGAL AND BACTERIAL ANTAGONISTS AS BIOINOCULANTS. Agricultural Science Digest. 34(4): 281 - 284. doi: 10.5958/0976-0547.2014.01020.9.

ABSTRACT

The talc based formulations of two fungal (Trichoderma harzianum and T. hamatum) and seven bacterial antagonists (Strains of Bacillus subtilis and Pseudomonas fluorescens) were evaluated for their shelf life at two temperatures (ambient and 10oC). Microbial count was highest initially but a gradual decline was recorded with the increase in the storage time. Among fungal and bacterial antagonists, highest microbial count was recorded in T. harzianum (2.2 x 108) and Bacillus subtilis I2 (2.0 x 108) at zero day which was reduced to 8.3 x 106 cfu/g and 5.6 x 106 cfu/g, respectively, after 80 days of storage at ambient temperature. At 10oC, initial highest count was found in T. harzianum (2.0 x 108) and P. fluorescens I1 (1.2 x 108) which was declined to 3.0 x 106 and 3.3 x 106, respectively, after 80 days of storage.

REFERENCES

  1. Bhat, K.A., Anwar, A., Lone, G.M., Hussain, K. and Nazir, G. (2009). Shelf life of liquid fermented product of Trichoderma harzianum in talc. J. Mycol. Pl. Pathol. 39(2): 263-265.
  2. El-Hassan, S.A. and Gowen, S.R. (2006). Formulation and delivery of the bacterial antagonist Bacillus subtilis for management of lentil vascular wilt caused by Fusarium oxysporum f.sp. lentis. J. Phytopathology 154 (3): 148–155.
  3. Gade, R.M., Wardhe, S.R., Armarker, S., Sangale, U.R. and Sharma, P.K. (2008). Shelf life of Trichoderma spp. in different carrier materials. J. Mycol. Pl. Pathol. 38(3): 606-607.
  4. Jayaraj, J., Parthasarthy, T. and Radhakrishnan, N.V. (2007). Characterization of a Pseudomonas fluorescens strain from tomato rhizosphere and its use for integrated management of tomato damping-off. Biocontrol 52(5): 683-702.
  5. Jayaraj, J., Radhakrishnan, N.V. and Velazhahan, R. (2006). Development of formulations of Trichoderma harzianum strain M1 for control of damping-off of tomato caused by Pythium aphanidermatum. Archives Phytopathology Plant Protec. 39(1): 1-8.
  6. Jayaraj, J., Radhakrishnan, N.V., Kannan, R., Sakthivel, K. Suganya, D., Venkatesan, S. and Velazhahan, R. (2005). Development of new formulations of Bacillis subtilis for management of tomato damping-off caused by Pythium aphanidermatum. Biocontrol Sci Technol. 15(1): 55-65.
  7. Jayarajan, R., Ramakrishnan, G., Dinakaran, D. and Sridhar, R. (1994). Development of product of Trichoderma viride and Bacillus subtilis for biocontrol of root rot disease. In: Biotechnology in India. Dwivedi, B.K. (Eds) pp 25-36. Bioved Research Society, Allahabad.
  8. Kloepper, J.W. and Schroth, M.N. (1981). Development of powder formulation of rhizobacteria for inoculation of potato seed pieces. Phytopathology 71: 590–592.
  9. Kumaresan, K., Subramanian, M., Vaithiyanathan, S., Sevagaperumal, N. and Chandradekar, G. (2005). Broad spectrum action of phenazine against active and dormant structures of fungal pathogens and root knot nematode. Archives Phytopathology Plant Protec. 38(1): 19-30.
  10. Mythukumar, A. (2009). Trichoderma viride and Pseudomonas fluorescens formulations against Pythium aphanidermatum in vitro. Indian J. Plant Protec. 37(1/2):204-206.
  11. Nakkeran, S., Sankar, P. and Jeyarajan, R. (1997). Standardization of storage conditions to increase the shelf life of Trichoderma formulations. J. Mycol. Pl. Pathol. 27: 60-63.
  12. Sabaratnam, S. and Traquair, J.A. (2002). Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biol. Contl. 23(3): 245-253.
  13. Sandikar, B.M. and Awasthi, R.S. (2010). Preparation and shelf-life study of Pseudomonas and Bacillus bioformulations against phytopathogenic Pythium and Fusarium species. Internat. J. Plant Protec. 2(2): 251-254.
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