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Current IssueEditorial BoardOnline First ArticlesArchive

volume 34 issue 3 (september 2014) : 203 - 206,   Doi: 10.5958/0976-0547.2014.01002.7

STUDIES ON GENETIC VARIABITY IN BASMATI AND NON BASMATI RICE (ORZYA SATIVA L.) USING RAPD MARKERS

R
Ravindra kumar*
P
Parteek Mahalwar
R
Ritu Sirohi
K
Kavita Jethuri
V
Vivek Kumar
1Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, India
Cite article:- kumar* Ravindra, Mahalwar Parteek, Sirohi Ritu, Jethuri Kavita, Kumar Vivek (2025). STUDIES ON GENETIC VARIABITY IN BASMATI AND NON BASMATI RICE (ORZYA SATIVA L.) USING RAPD MARKERS. Agricultural Science Digest. 34(3): 203 - 206. doi: 10.5958/0976-0547.2014.01002.7.

ABSTRACT

A total of 14 genotypes of rice (Oryza sativa L.) were subjected to random amplified polymorphic DNA (RAPD) analysis using polymerase chain reaction with ten arbitrary decamer oligonucleotide primers. Rice genotypes studied in current investigation include three traditional basmati gentypes, six evolved basmati varieties, and five varieties of non basmati rice. The percentage of polymorphic bands ranged from 71% to 100% with an average of 89% polymorphism. Fairly high genetic diversity was recorded through jaccard’s similarity matrix, the genetic similarity coefficient (GS) values based on RAPDs for all the possible pairs of genotypes ranged from 0.02 ( Haryana Basmati-1 and Narendra Usar-3) to 1.000 (Ranbir Basmati and Type -3). It was observed that genetic diversity is lower among the traditional basmati genotypes whereas the evolved basmati varieties showed the higher genetic diversity.

REFERENCES

  1. Chang, T.T. (1976). The origin, evolution, cultivation, dissemination and diversification of Asian and African rices. Euphytica., 25: 425-445.
  2. Choudhury, P.R.; Kohli, S.; Srinivasan, K.; Mohapatra, T. and Sharma, R.P. (2001) Identification and classification of aromatic rices based on DNA fingerprinting. Euphytica., 118: (3) 243-251.
  3. O’Neill, R.; Snowdon, R.J. and Kohler, W. (2003). Population genetics aspects of biodiversity. Progress in Botany.,
  4. 64: 115-137.
  5. Murray, M. G. and Thompson, W. F. (1980). Rapid isolation of high molecular weigh plant DNA. Nucleic Acids Res., 8: 4321-4325.
  6. Raychoudhury, P.; Kohli, S.; Srinivasan, K.; Mohapatra, T. Sharma, R. P. (2001) Identification and classification of aromatic rices based on DNA fingerprinting.Euphytica.,118: 243–251.
  7. Ren, F.; Lu, B.; Li, S.; Huang, J. and Zhu, Y. (2003) A comparative study of genetic relationships among the AA- genome Oryza species using RAPD and SSR markers. Theoretical and Applied Genetics.,108: (1) 113-120.
  8. Rohlf, F. J. (1993). Numerical taxonomy and multivarieties analysis system NTSys-PC version 1.80 Exeter software, New York.
  9. Rohlf, F. J. (2000). NTSYS-pc: Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publications, New York, USA.
  10. Saker, M.M.; Youssef, S.S.; Abdallah, N.A.; Bashandy, H.S. and El-Sharkawy, A.M. (2005) Genetic analysis of some Egyptian rice genotypes using RAPD, SSR and AFLP. African Journal of Biotechnology., 49: 882-890.        
  11. Verma, S.K.; Khanna, V. and Singh, N. (1999). Random amplified polymorphic DNA analysis of Indian scented basmati rice (Oryza sativa L.) germplasm for identification of variability and duplicate accessions, if any. Electrophoresis., 20: (8) 81786-1789.
  12. Virk, P.S.; Ford-Llyod, B.V.; Newbury, H.J. and Jackson, M.T. (1995). The identification of duplicate accessions within the rice germplasm collection using RAPD analysis., Theor. Appl. Genet. 90:1049-1055.
  13. Williams, K.; Kubelik, A.; Livak, K.; Rafalski, J. and Tingey, V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res., 18: 6531–6535.
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    volume 34 issue 3 (september 2014) : 203 - 206,   Doi: 10.5958/0976-0547.2014.01002.7

    STUDIES ON GENETIC VARIABITY IN BASMATI AND NON BASMATI RICE (ORZYA SATIVA L.) USING RAPD MARKERS

    R
    Ravindra kumar*
    P
    Parteek Mahalwar
    R
    Ritu Sirohi
    K
    Kavita Jethuri
    V
    Vivek Kumar
    1Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, India
    Cite article:- kumar* Ravindra, Mahalwar Parteek, Sirohi Ritu, Jethuri Kavita, Kumar Vivek (2025). STUDIES ON GENETIC VARIABITY IN BASMATI AND NON BASMATI RICE (ORZYA SATIVA L.) USING RAPD MARKERS. Agricultural Science Digest. 34(3): 203 - 206. doi: 10.5958/0976-0547.2014.01002.7.

    ABSTRACT

    A total of 14 genotypes of rice (Oryza sativa L.) were subjected to random amplified polymorphic DNA (RAPD) analysis using polymerase chain reaction with ten arbitrary decamer oligonucleotide primers. Rice genotypes studied in current investigation include three traditional basmati gentypes, six evolved basmati varieties, and five varieties of non basmati rice. The percentage of polymorphic bands ranged from 71% to 100% with an average of 89% polymorphism. Fairly high genetic diversity was recorded through jaccard’s similarity matrix, the genetic similarity coefficient (GS) values based on RAPDs for all the possible pairs of genotypes ranged from 0.02 ( Haryana Basmati-1 and Narendra Usar-3) to 1.000 (Ranbir Basmati and Type -3). It was observed that genetic diversity is lower among the traditional basmati genotypes whereas the evolved basmati varieties showed the higher genetic diversity.

    REFERENCES

    1. Chang, T.T. (1976). The origin, evolution, cultivation, dissemination and diversification of Asian and African rices. Euphytica., 25: 425-445.
    2. Choudhury, P.R.; Kohli, S.; Srinivasan, K.; Mohapatra, T. and Sharma, R.P. (2001) Identification and classification of aromatic rices based on DNA fingerprinting. Euphytica., 118: (3) 243-251.
    3. O’Neill, R.; Snowdon, R.J. and Kohler, W. (2003). Population genetics aspects of biodiversity. Progress in Botany.,
    4. 64: 115-137.
    5. Murray, M. G. and Thompson, W. F. (1980). Rapid isolation of high molecular weigh plant DNA. Nucleic Acids Res., 8: 4321-4325.
    6. Raychoudhury, P.; Kohli, S.; Srinivasan, K.; Mohapatra, T. Sharma, R. P. (2001) Identification and classification of aromatic rices based on DNA fingerprinting.Euphytica.,118: 243–251.
    7. Ren, F.; Lu, B.; Li, S.; Huang, J. and Zhu, Y. (2003) A comparative study of genetic relationships among the AA- genome Oryza species using RAPD and SSR markers. Theoretical and Applied Genetics.,108: (1) 113-120.
    8. Rohlf, F. J. (1993). Numerical taxonomy and multivarieties analysis system NTSys-PC version 1.80 Exeter software, New York.
    9. Rohlf, F. J. (2000). NTSYS-pc: Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publications, New York, USA.
    10. Saker, M.M.; Youssef, S.S.; Abdallah, N.A.; Bashandy, H.S. and El-Sharkawy, A.M. (2005) Genetic analysis of some Egyptian rice genotypes using RAPD, SSR and AFLP. African Journal of Biotechnology., 49: 882-890.        
    11. Verma, S.K.; Khanna, V. and Singh, N. (1999). Random amplified polymorphic DNA analysis of Indian scented basmati rice (Oryza sativa L.) germplasm for identification of variability and duplicate accessions, if any. Electrophoresis., 20: (8) 81786-1789.
    12. Virk, P.S.; Ford-Llyod, B.V.; Newbury, H.J. and Jackson, M.T. (1995). The identification of duplicate accessions within the rice germplasm collection using RAPD analysis., Theor. Appl. Genet. 90:1049-1055.
    13. Williams, K.; Kubelik, A.; Livak, K.; Rafalski, J. and Tingey, V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res., 18: 6531–6535.
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