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Research Article

Agricultural Science Digest, volume 40 issue 4 (december 2020) : 418-423

Effect of Vitamin D on the Growth, Haematological and Bio-Chemical Profile of Labeo rohita

Muhammad Inayat, Farzana Abbas, Saira Razzaq, Muhammad Hafeez-ur-Rehman, Arshad Javid, Ali Hussain, Muhammad Akmal, Iqra Anwer
1Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Cite article:- Inayat Muhammad, Abbas Farzana, Razzaq Saira, Hafeez-ur-Rehman Muhammad, Javid Arshad, Hussain Ali, Akmal Muhammad, Anwer Iqra (2020). Effect of Vitamin D on the Growth, Haematological and Bio-Chemical Profile of Labeo rohita. Agricultural Science Digest. 40(4): 418-423. doi: 10.18805/ag.D-226.

ABSTRACT

Background: Fish health and disease management is the key factor in modern aquaculture where intensification manifolds the infestation chances of parasites. Irrespective of antibiotics, the use of dietary additives gaining attention in development of aqua feed. Vitamin D is an important dietary additive for fish in terms it can boost up the immune system of fish and unluckily fish cannot synthesize vitamin D itself. 
Methods: The present study was planned to determine the effect of vitamin D as feed additive on the immunity, hematology and body composition of Labeo rohita. Four diets (T1, T2, T3, T4) containing different concentration of vitamin. D (250mg, 500mg, 1.0g and 1.5g) in food and a control diet were administrated for 90 days trial. 
Result: At the termination of experiment there were significant differences in growth and hematological parameters between control and test diets. T3 (1.0g of vitamin D) showed the maximum weight gain with lowest FCR value (1.43±0.33) however, the RBC count of T4 group was highest (1.85±0.07) than the control, T1, T2 and T3 groups.  The body composition of Labeo rohita growth factor and survival rate were significantly higher (P < 0.05) in fish fed on diets containing vitamin D at 1.00g/Kg concentration.

REFERENCES

  1. Andrews, J. W., Murai, T., Page, J. W. (1980). Effects of dietary cholecalciferol and ergocalciferol on catfish. Aquaculture. 19(1): 49-54. 
  2. AOAC., (2010). Official Methods of Analysis of Association of Official Analytical Chemists. 18th Edition, Washington, DC.
  3. Bahmani, M., Kazemi, R., Donskaya, P. (2001). A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiology and Biochemistry. 24(2):135-140. 
  4. Bekcan, S., Dogankaya, L., Cakirogullari, G. C. (2006). Growth and body composition of European catfish (Silurus glanis L.) fed diets containing different percentages of protein. Israeli Journal Aquaculture. Bamidgeh. 58: 137-142.
  5. Bhosale, S.V., Bhilave, M.P., Nadaf, S.B. (2010). Formulation of fish feed using ingredients from plant sources. Research Journal of Agricultural Sciences. 1(3): 284-287.
  6. Blaxhall, P.C., Daisley, K.W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology. 5(6):771-781. 
  7. Brown, P.B. and Robinson, E.H. (1992). Vitamin D studies with channel catfish (Ictalurus punctatus) reared in calcium-free water. Comparative Biochemistry and Physiology Part A: Physiology. 103(1): 213-219. 
  8. Calvo, M.S., Whiting, S.J., Barton, C.N. (2005). Symposium: vitamin D insufficiency: a significant risk factor in chronic diseases and potential disease-specific biomarkers of vitamin D sufficiency. Journal of Nutrition. 135: 301-37.
  9. Cnaani, A., Tinman, S., Avidar, Y., Ron, M., Hulata, G. (2004). Comparative study of biochemical parameters in response to stress in Oreochromis aureus, O. mossambicus and two strains of O. niloticus. Aquaculture Research. 35(15): 1434-1440. 
  10. Cranney, A., Horsley, T., O’Donnell, S., Weiler, H., Puil, L., Ooi, D., Fang, M. (2007). Effectiveness and safety of vitamin D in relation to bone health. Evidence report/technology assessment. (158): 1.
  11. Dacie, J. A., Lewis, S. M. (1991). Practical haematology A (7th ed.). London: Churchill Livingstone.
  12. Andrade, J. I. A., Ono, E. A., de Menezes, G. C., Brasil, E. M., Roubach, R., Urbinati, E. C., Affonso, E. G. (2007). Influence of diets supplemented with vitamins C and E on pirarucu (Arapaima gigas) blood parameters. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 146(4): 576-580. 
  13. Dusso, A.S., Brown, A.J., Slatopolsky, E. (2005) Vitamin D. Am. J. Physiol. Renal Physiol, 289: 8-28. 
  14. Falahatkar, B. (2005). The effect of dietary vitamin C on some of hematologhc biochemistry and growth indexes of great sturgeon (Huso huso) (Doctoral dissertation, Ph. D. Thesis, Tarbiat Modaarres University, Tehran, Iran (Persian).
  15. Gasco, L., Gai, F., Maricchiolo, G., Genovese, L., Ragonese, S., Bottari, T., Caruso, G. (2018). Supplementation of Vitamins, Minerals, Enzymes and Antioxidants in Fish Feeds. In Feeds for the Aquaculture Sector. 63-103. Springer, Cham.
  16. Graff, I. E., Høie, S., Totland, G. K., Lie, Ø. (2002). Three different levels of dietary vitamin D3 fed to first feeding fry of Atlantic salmon (Salmo salar L.): effect on growth, mortality, calcium content and bone formation. Aquaculture Nutrition. 8(2):103-111. 
  17. Guimarães, I.G., Lim, C., Yildirim-Aksoy, M., Li, M.H., Klesius, P.H. (2014). Effects of dietary levels of vitamin A on growth, hematology, immune response and resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae. Animal Feed Science and Technology. 188: 126-136. 
  18. Hilton, J.W. and Ferguson, H.W. (1982). Effect of excess vitamin D3 on calcium metabolism in rainbow trout Salmo gairdneri Richardson. Journal of Fish Biology. 21(4): 373-379. 
  19. Horvli, O., Aksnes, L., Lie, Ø. (1998). Tissue distribution of vitamin D3 in Atlantic salmon Salmo salar: effect of dietary level. Aquaculture Nutrition. 4(2): 127-132. 
  20. Ibrahem, M.D., Fathi, M., Mesalhy, S., El-Aty, A.A. (2010). Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity and resistance of Nile tilapia (Oreochromis niloticus). Fish and Shellfish Immunology. 29(2): 241-246. 
  21. Jhingran, V.G. and Pullin, R.S. (1985). A hatchery manual for the common, Chinese and Indian major carps (No. 252). World Fish.
  22. Khan, M.A., Ahmed, I., Abidi, S.F. (2004). Effect of ration size on growth, conversion efficiency and body composition of fingerling mrigal, Cirrhinus mrigala (Hamilton). Aquaculture Nutrition. 10(1): 47-53. 
  23. Lim, C., Klesius, P.H., Li, M.H., Robinson, E.H. (2000). Interaction between dietary levels of iron and vitamin C on growth, hematology, immune response and resistance of channel catfish (Ictalurus punctatus) to Edwardsiella ictalurichallenge. Aquaculture. 185(3-4):313-327.
  24. Lin, M.F. and Shiau, S.Y. (2005). Dietary L-ascorbic acid affects growth, nonspecific immune responses and disease resistance in juvenile grouper, Epinephelus malabaricus. Aquaculture. 244(1-4):215-221. 
  25. Lock, E.J., R. Waagbø, S. Wendelaar Bonga, G. Flik, (2010). The significance of vitamin D for fish: a review. Aquaculture Nutrition. 16(1): 100-116. 
  26. Montero, D., Tort, L., Robaina, L., Vergara, J.M., Izquierdo, M.S. (2001). Low vitamin E in diet reduces stress resistance of gilthead seabream (Sparus aurata) juveniles. Fish and Shellfish Immunology. 11(6): 473-490. 
  27. Poston, H.A. (1968). Effects of massive doses of vitamin D3 on fingerling brook trout. Fish. Res. Bull. 32: 48-50.
  28. Rahman, M.M., Verdegem, M.C.J., Nagelkerke, L.A.J., Wahab, M.A., Milstein, A., Verreth, J.A.J. (2006). Growth, production and food preference of rohu Labeo rohita (H.) in monoculture and in polyculture with common carp Cyprinus carpio (L.) under fed and non-fedponds. Aquaculture. 257(1-4): 359-372. 
  29. Rambhaskar, B., Srinivasa Rao, K. (1987). Comparative haematology of ten species of marine fish from Visakhapatnam Coast. Journal of Fish Biology. 30(1): 59-66. 
  30. Ross, A.C. (2011). The 2011 report on dietary reference intakes for calcium and vitamin D. Public Health Nutrition. 14(5): 938-939. 
  31. Svobodova, Z., Kroupova, H., Modra, H., Flajšhans, M., Randak, T., Savina, L.V., Gela, D. (2008). Haematological profile of common carp spawners of various breeds. Journal of Applied Ichthyology. 24(1): 55-59. 
  32. Takeuchi, A., Okano, T., Torii, M., Hatanaka, Y., Kobayashi, T. (1987). Comparative studies on the contents of vitamin D3, 25-hydroxy vitamin D3 and 7-dehydrocholesterol in fish liver. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 88(2): 569-573. 
  33. Tatina, M., Bahmani, M., Soltani, M., Abtahi, B., Gharibkhani, M. (2010). Effects of different levels of dietary vitamins C and E on some of hematological and biochemical parameters of sterlet (Acipenser ruthenus). Journal Fish Aquatic Science. 5: 1-11. 
  34. Taveekijakarn, P., Miyazaki, T., Matsumoto, M., Aral, S. (1996). Histopathological and haematological changes in amago salmon, Oncorhynchus rhodurus (Jordan and McGregor), fed a vitamin D free diet. Journal of Fish Diseases. 19(4): 289-294. 
  35. Xiaoyun, Z., Mingyun, L., Khalid, A., Weinmin, W. (2009). Comparative of haematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicadatus. Fish Physiology Biochemistry. 35: 435-441.
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