Agricultural Science Digest
Publish
your articles with us

Quick Facts



Payment Options

payment portals

Click here to pay directly

In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

A.S. Abubakar, S.U. Yahaya, A.S. Shaibu, H. Ibrahim, A.K. Ibrahim, Z.M. Lawan and A.M. Isa
Department of Agronomy, Bayero University, Kano, P.M.B 3011, Kano-Nigeria
aashehu.agr@buk.edu.ng

Page Range:
17-21
Article ID:
D-128
Online Published:
10-03-2018
Abstract
The study is aimed at establishing a simple protocol for in vitro regeneration of sweet potato with a view to providing planting materials to farmers as well as basis for genetic improvement. Axillary buds were excised and cultured on Murashige and Skoog (MS) basal salts supplemented with 6-benzyl aminopurine (BAP), gibberellic acid (GA3) and naphthalene acetic acid (NAA) singly or in combination. The shoot height and number of leaves differed significantly among the cultivars. The result also indicated significant difference (p< 0.01) among the cultivars with King J recording the highest mean values. Significant differences (p< 0.05) was also recorded in the media combination with respect to organogenesis and number of shoots obtained. The results of hardening further revealed 33.33% success in the explants transferred directly to the field, as well as for the plantlets that were gradually weaned in a mixture of 3:1 sand and biochar.
Keywords
Gibberellic acid, Murashige and Skoog, Naphthalene acetic acid, Sweet potato, 6-benzyl amino purine
References
  1. Addae-Frimpomaah, F., Amponsah, J., and Tengey, T. K. (2014). Regeneration of three sweet potato (Ipomoea batatas L.) accessions in Ghana via meristem and nodal culture. International Journal of Plant Breeding and Genetics. 8(3):121 – 138. DOI: 10.3923ijpbg.2014.121.138.
  2. Adikini, S., Settumba, B. M., Mwanga, R. O. M., and Gibson, R.W. (2015). Sweet potato cultivar degeneration rate under high and low potato virus disease pressure zones in Uganda. Canadian Journal of Plant Pathology, 37(1): 136-147.
  3. Aloufa, M. A. (2002). Some factors affecting the callus induction and shoot formation in two cultivars of Sweet potato (Ipomoea batatas L. POIS). Cienciae Agrotecnologia 26: 964–969.
  4. ASARECA (2008). Enhancement of cassava and sweet potato tissue culture and conservation technologies in the Eastern and Central Africa region.
  5. Barker, I., Andrade, M., Labarta, R., Mwanga, R., Kapinga, R., Fuentes, S. and Low, J. (2011). Sustainable seed systems. International Potato Center (CIP). 
  6. Delgado-Sanchez, P., Saucedo-Ruiz, M., Guzmán-Maldonado, S. H., Villordo-Pineda, E., González-Chavira, M., et al. (2006). An organogenic plant regeneration system for common bean (Phaseolus vulgaris L.). Plant Science 170(4):822-827.
  7. Doliñski, R. and Olek, O. (2013) Micropropagation of sweet potato (Ipomoea batatas (L.) Lam.) from node explants . Acta Sci Pol., Hortorum Cultus 12(4): 117-127
  8. Dugassa, G. and Feyissa, T. (2011) In vitro production of virus-free sweet potato (Ipomoea batatas (L.) LAM) by meristem culture and thermotherapy. SINET: Ethiop. Journal of Science. 34(1): 17 – 28.
  9. El-Afifi, S. T, Zaghloul, M. M., El Saady, W. A. and Mosaad, F. S. (2012) Using tissue culture technique in micropropagation of sweet potato (Ipomoea batatas). Journal of Plant Production, Mansoura Univ. 3(7): 2201 - 2209, 2012
  10. FAO (2008). FAO Statistical Databases. Food and Agriculture Organization of the United Nations. http://faostat.fao.org
  11. FAO (2011). FAO Statistical Databases. Food and Agriculture Organization of the United Nations. http://faostat.fao.org
  12. Gaba, V. P. (2005). Plant growth regulators in plant tissue culture and development. In Plant development and biotechnology, [Trigiano, R. N, Gray, D. J (eds)]. CRC Press. Boca Ratom: 87-99.
  13. Gubba, A. and Sivparsad, B. J. (2002). Development of an efficient plant regeneration protocol for sweet potato (Ipomoea batatas L.) cv. Blesbok. African Journal of Biotechnology. 11(84):14982-14987.
  14. Islam S. (2006). Sweet potato (Ipomoea batatas L.) leaf: its potential effect on human health and nutrition. J. Food Sci. 71: 2-13.
  15. Kapinga, R., Ortiz, O., Ndunguru, J., Omiat, E. and Tumwegamire. (2007). Handbook of Sweet potato Integrated Crop Management. Research Outputs and Programmes for East Africa (1995-2006). International Potato Center (CIP). pp. 5-7.
  16. Mukherjee, A. (2002) Effect of NaCl on In Vitro Propagation of Sweet Potato (Ipomoea batatas L.). Applied Biochemistry and Biotechnology. 102–103: 431-441
  17. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  18. Namanda, S., Gibson, R.W. and Kirimi, S., (2011). Sweet potato seed systems in Uganda, Tanzania and Rwanda. Journal of Sustainable Agriculture. 35: 870-884. 
  19. Ogero, K. O., Gitonga, N. M., Mwangi, M. Ombori, O. and Ngugi, M. (2011). A low-cost medium for sweet potato micro propagation. African Crop Science Conference Proceedings, 10: 57-63.
  20. Onwubiko, N. C., Ihezie, C. I. and Mozie, M. U. (2015). In vitro Regeneration of Sweet Potato (Ipomoea batatas (L.) Lam.) from Node Explants. American Journal of Experimental Agriculture. 8(2): 87-92.
  21. Pfeiffer, W.H. and Mclafferty, B. (2007). Harvest-Plus: Breeding crops for better nutrition. Crop Science 47: 88 – 105.
  22. SAS (2015). SAS Institute Inc. SAS University Edition: Installation Guide for Windows. Cary, NC: SAS Institute.
  23. Shaibu, A. S., Abubakar, A. S., Lawan, Z. M., Ibrahim, A. K., Rabiu, H. M., Muhammad, A. I., et al. (2016). Media Optimization and Effect of Surface Sterilization Timing on In Vitro Propagation of Sweet Potato. Proceedings of the 2nd International Conference on Drylands.
  24. Sihachakr, D., Haïcour, R., Cavalcante Aves, J. M., Umboh, I., Nzoghé, D., Servaes, A.,Ducreux, G. (1997) Plant regeneration in sweet potato (Ipomoea batatas L., Convolvulaceae). Euphytica. 96(1):143-152.
  25. Sivparsad, B. J and Gubba, A. (2012). Development of an efficient plant regeneration protocol for sweet potato (Ipomoea batatas L.) cv. Blesbok. African Journal of Biotechnology 11(84): 14982-14987.
  26. Thorpe T. A. (1994) Morphogenesis and regeneration. In Plant Cell and Tissue Culture, [Thorpe TA, Vasil, IK (eds)] Kluwer Academic Publishers. Netherlands, Dordrecht 7-36. 
  27. Tumwegamire, S., Kapinga, R., Rubaihayo, P. R., LaBonte, D. R., Grüneberg, W. J., Burgos, G., et al., (2011). Evaluation of dry matter, protein, starch, sucrose, ß-carotene, iron, zinc, calcium, and magnesium in East African sweet potato (Ipomoea batatas (L.) Lam) germplasm. HortSci. 46(3): 348–357.
  28. U.S.D.A (2007). USDA National Nutrient Database for Standard Reference, Release 20. http://www.nal.usda.gov./fnic/foodcomp/    search 
Global footprints


© 2015 ARCC JOURNALS. All Rights Reserved. Powered By ARCC JOURNALS