A field experiment was conducted to study the harmful effect of salinity on physiological, biochemical traits, growth and yield of wheat genotypes namely, Raj-3077, Raj-3765 (salt tolerant) and Raj-1482, PBW-502 (salt susceptible), were grown in cemented pots under different levels of salinity (0, 4.0, 6.0 and 8.0 dSm-1) at 30 DAS, 60 DAS and anthesis stage. Salinity was found to decrease significantly the photosynthesis rate, transpiration rate, stomatal conductance, relative water content, chlorophyll content, osmotic potential, soluble sugar content, plant height, leaf area, number of leaves, number of grains, total number of tillers/plant, test weight, fresh, and dry weight, grain yield, biological yield, and K+ content in grain with an increase in proline content, membrane injury, Na+ content in grain in all the genotypes .The genotypic variations pertaining to these observations were also found significant. The magnitude of decrease in these parameters under salinity was different at different stages of observation. However, the trend was almost similar at all the stages. Salinity tolerant genotypes show a lesser decline in these variables except proline, membrane injury, and Na+ content when compared to salinity susceptible cultivars. Genotype Raj-3077, Raj-3765 was recorded to having the highest value of all these parameters than Raj-1482, PBW-502 except osmotic potential, the value of which was seen highest in PBW-502. The maximum decline in these parameters was recorded at the salinity level of 8.0 dSm-1.