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Current IssueEditorial BoardOnline First ArticlesArchive

volume 33 issue 3 (september 2013) : 211-214,   Doi: 10.5958/j.0976-0547.33.3.010

AN EFFICIENT PROTOCOL ON PLANT REGENERATION OF UPLAND INDICA RICE (ORYZA SATIVA VARS. GHANTESHWARI AND SIDDHANTA)

S
Sushil P. Shirsat*
G
G.R. Rout
1Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar- 751 003, India
Cite article:- Shirsat* P. Sushil, Rout G.R. (2025). AN EFFICIENT PROTOCOL ON PLANT REGENERATION OF UPLAND INDICA RICE (ORYZA SATIVA VARS. GHANTESHWARI AND SIDDHANTA). Agricultural Science Digest. 33(3): 211-214. doi: 10.5958/j.0976-0547.33.3.010.

ABSTRACT

An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two varieties of indica rice i.e.  Ghanteshwari and Siddhanta. Shoot apical meristems from 7-d-old seedlings produced axillary and adventitious shoots on MS media containing 3.0 mg/l 6-benzyladenine (BA). Both the genotypes responded positively on shoot multiplication depending upon media composition. The maximum rate of  shoot multiplication was 90%, and the average number of multiple shoots per culture was 12.5  in case of var. Ghanteswari and 10.6 in case of  Siddhanta  within 4 weeks of culture on 3.0 mg /l BA in combination with 50 mg/l adenine sulphate (Ads). Actively multiplying shoot clumps were recovered with high frequency within 4 weeks of cultures. These excised shoots were rooted on MS medium supplemented with 0.5 mg/l NAA and 3% sucrose. Plantlets were success-fully established in soil. This efficient protocol may be utilized for the production of elite clones and  also genetic improvement program.

REFERENCES

  1. Benjamins R. and Scheres B. (2008) Auxin: the looping star in plant development. Annu. Rev. Plant Biol. 59:443–465.
  2. Dorosieve, L. (1996) Plant cell and tissue cultures present state and future prospects. Genet. Selektasiya 19:356–362
  3. Greco, B.; Lomonaco, A. and Boggi’ni, B. (1990) Clonal propagation of rice through proliferation of axillary shoots. Euphytica 48:123-127.
  4. Kurakawa, T., Ueda, N., Maekawa, M., Kobayashi, K., Kojima, M., Nagato, Y.,Sakakibara, H., and Kyozuka, J. (2007). Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature 445: 652–655.
  5. Medford, J. I. (1992) Vegetative apical meristems. Plant Cell 4:1029–1039.
  6. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473–497.
  7. Peret, B., De Rybel, B., Casimiro, I., Benkova, E., Swarup, R., Laplaze, L., Beeckman, T. and Bennett, M.J. (2009) Arabidopsis lateral root development: an emerging store, Trends Plant Sci. 14:399–408.
  8. Rebouillat J., Dievart A., Verdeil J.L., Escoute J., Giese G., Breitler J.C., Gantet P., Espeout S., Guiderdoni E., and Perin C. (2009) Molecular genetics of rice root development. Rice 2:15–34.
  9. Reinhardt, D., Mandel, T. and Kuhlemeier, C. (2000) Auxin regulates the initiation and radial position of plant lateral organs, Plant Cell, 12:507–518.
  10. Sandhu, J. S.; Gosal, S. S. and Gill, M. S. (1995) Micropropagation of indica rice through proliferation of axillary shoots. Euphytica 81:139-142.
  11. Shimizu-Sato, S., Tanaka, M., Mori, H. (2009) Auxin-cytokinin interactions in the control of shoot branching. Plant Mol. Biol. 69:429–435.
  12. Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., Mori, H. ( 2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J. 45: 1028– 1036.
  13. Zazimalova, E., Murphy, A.S., Yang, H., Hoyerova, K., Hosek, P. (2009) Auxin transporters–why so many? Cold Spring Harb. Perspect. Biol. (December) doi:10.1101/cshperspect.a001552.
  14. Zhang S.; Zhong H. and Sticklen M. B. (1996) Production of multiple shoots from shoot apical meristems of oat (Avena sativa L.). J. Plant Physiol. 148:667–671.
  15. Zhong, H.; Srinivasan, C. and Sticklen, M. B (1992) In vitro mophogenesis of corn (Zea mays L.). Planta 187:483– 489.
  16. Zhong, H.; Wang, W. and Sticklen, M. B. (1998) In vitro morphogenesis of Sorghum bicolor (L.) Moench: efficient plant regeneration from shoot apices. J. Plant Physiol. 153:719–726.
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    volume 33 issue 3 (september 2013) : 211-214,   Doi: 10.5958/j.0976-0547.33.3.010

    AN EFFICIENT PROTOCOL ON PLANT REGENERATION OF UPLAND INDICA RICE (ORYZA SATIVA VARS. GHANTESHWARI AND SIDDHANTA)

    S
    Sushil P. Shirsat*
    G
    G.R. Rout
    1Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar- 751 003, India
    Cite article:- Shirsat* P. Sushil, Rout G.R. (2025). AN EFFICIENT PROTOCOL ON PLANT REGENERATION OF UPLAND INDICA RICE (ORYZA SATIVA VARS. GHANTESHWARI AND SIDDHANTA). Agricultural Science Digest. 33(3): 211-214. doi: 10.5958/j.0976-0547.33.3.010.

    ABSTRACT

    An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two varieties of indica rice i.e.  Ghanteshwari and Siddhanta. Shoot apical meristems from 7-d-old seedlings produced axillary and adventitious shoots on MS media containing 3.0 mg/l 6-benzyladenine (BA). Both the genotypes responded positively on shoot multiplication depending upon media composition. The maximum rate of  shoot multiplication was 90%, and the average number of multiple shoots per culture was 12.5  in case of var. Ghanteswari and 10.6 in case of  Siddhanta  within 4 weeks of culture on 3.0 mg /l BA in combination with 50 mg/l adenine sulphate (Ads). Actively multiplying shoot clumps were recovered with high frequency within 4 weeks of cultures. These excised shoots were rooted on MS medium supplemented with 0.5 mg/l NAA and 3% sucrose. Plantlets were success-fully established in soil. This efficient protocol may be utilized for the production of elite clones and  also genetic improvement program.

    REFERENCES

    1. Benjamins R. and Scheres B. (2008) Auxin: the looping star in plant development. Annu. Rev. Plant Biol. 59:443–465.
    2. Dorosieve, L. (1996) Plant cell and tissue cultures present state and future prospects. Genet. Selektasiya 19:356–362
    3. Greco, B.; Lomonaco, A. and Boggi’ni, B. (1990) Clonal propagation of rice through proliferation of axillary shoots. Euphytica 48:123-127.
    4. Kurakawa, T., Ueda, N., Maekawa, M., Kobayashi, K., Kojima, M., Nagato, Y.,Sakakibara, H., and Kyozuka, J. (2007). Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature 445: 652–655.
    5. Medford, J. I. (1992) Vegetative apical meristems. Plant Cell 4:1029–1039.
    6. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473–497.
    7. Peret, B., De Rybel, B., Casimiro, I., Benkova, E., Swarup, R., Laplaze, L., Beeckman, T. and Bennett, M.J. (2009) Arabidopsis lateral root development: an emerging store, Trends Plant Sci. 14:399–408.
    8. Rebouillat J., Dievart A., Verdeil J.L., Escoute J., Giese G., Breitler J.C., Gantet P., Espeout S., Guiderdoni E., and Perin C. (2009) Molecular genetics of rice root development. Rice 2:15–34.
    9. Reinhardt, D., Mandel, T. and Kuhlemeier, C. (2000) Auxin regulates the initiation and radial position of plant lateral organs, Plant Cell, 12:507–518.
    10. Sandhu, J. S.; Gosal, S. S. and Gill, M. S. (1995) Micropropagation of indica rice through proliferation of axillary shoots. Euphytica 81:139-142.
    11. Shimizu-Sato, S., Tanaka, M., Mori, H. (2009) Auxin-cytokinin interactions in the control of shoot branching. Plant Mol. Biol. 69:429–435.
    12. Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., Mori, H. ( 2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J. 45: 1028– 1036.
    13. Zazimalova, E., Murphy, A.S., Yang, H., Hoyerova, K., Hosek, P. (2009) Auxin transporters–why so many? Cold Spring Harb. Perspect. Biol. (December) doi:10.1101/cshperspect.a001552.
    14. Zhang S.; Zhong H. and Sticklen M. B. (1996) Production of multiple shoots from shoot apical meristems of oat (Avena sativa L.). J. Plant Physiol. 148:667–671.
    15. Zhong, H.; Srinivasan, C. and Sticklen, M. B (1992) In vitro mophogenesis of corn (Zea mays L.). Planta 187:483– 489.
    16. Zhong, H.; Wang, W. and Sticklen, M. B. (1998) In vitro morphogenesis of Sorghum bicolor (L.) Moench: efficient plant regeneration from shoot apices. J. Plant Physiol. 153:719–726.
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