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Current IssueEditorial BoardOnline First ArticlesArchive

volume 33 issue 2 (june 2013) : 92-97

BIOCHEMICAL CHARACTERISTICS IMPARTING RESISTANCE AGAINST ALTERNARIA BLIGHT IN CAULIFLOWER GENOTYPES

M
Madhu Dhingra*
N
Neelima Arora
I
I.S. Aujla1
1Department of Botany Punjab Agricultural University, Ludhiana- 141 004, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Dhingra* Madhu, Arora Neelima, Aujla1 I.S. (2025). BIOCHEMICAL CHARACTERISTICS IMPARTING RESISTANCE AGAINST ALTERNARIA BLIGHT IN CAULIFLOWER GENOTYPES . Agricultural Science Digest. 33(2): 92-97. doi: .

ABSTRACT

Alternaria blight disease has been reported from all the continents of the world, affects most of the cruciferous crops. Alternaria blight incited by foliar pathogen, Alternaria brassicae has become a major constraint in the successful cultivation of this crop. Development of resistant varieties is the most appropriate approach to control the disease and the concept is now developing to explore the built-in plant defense mechanism in relation to pathogen attack. In the present study, an attempt was made to identify biochemical characteristics imparting resistance against Alternaria blight in cauliflower genotypes. The moderately resistant genotypes had higher total chlorophyll, total phenols, total soluble sugars, total proteins, flavonoids and ascorbic acid than the susceptible genotypes. The chlorophyll content was found to be lesser in the infected leaves than the healthy ones. The reduction in total phenols, total soluble sugars, total proteins, flavonoids and ascorbic acid was at a higher rate in susceptible ones than the moderately resistant genotypes. These biochemical characteristics might be responsible for imparting resistance against Alternaria blight in cauliflower genotypes.

REFERENCES

  1. Ammajamma R and Patil P V (2008) Biochemical factors imparting rust (Phakospora pachyrhizi) resistance in soybean. Karnataka J Agric Sci 21: 65-69.
  2. Arora N (2004) Role of phytoanticipins in resistance against red rot of sugarcane (Saccharum officinarum L.). Env & Eco 22: 861-63.
  3. Balabaa S I, Zake A Y and Elshamy A M (1974) Total flavonoid and rutin content of the different organs of Sophora japonica L. J Assoc Analyt Chem 57: 752-55.
  4. Chakrabarty P K, Mukewar P M, Raj S and Sravan Kumar V (2002) Biochemical factors governing resistance in diploid cotton against grey mildew. Indian Phytopath 55: 140-46.
  5. Chattopadhyay A K and Bagchi B N (1994) Relationship of disease severity and yield due to leaf blight of mustard and spray schedule of mancozels for higher yield. J Mycopath Res 32: 83-87.
  6. Conn K L, Tewari J P and Awasthi R P (1990) A disease assessment key for Alternaria blackspot in rapeseed and mustard. Can Pl Dis Surv 70: 19-22.
  7. Dubois M, Gilles K A, Hamilton J K, Roberts P A and Smith F (1956) Colorimetric methods for the determination of sugars and related substances. Anal Chem 28: 352-56.
  8. Ghose L, Neela F A, Chakravorty T C, Ali M R and Alam M S (2010) Incidence of leaf blight disease of mulberry plant and assessment of changes in amino acids and photosynthetic pigments of infected leaf. Plant Pathol J 95: 140-143.
  9. Guleria S, Paul B and Bajaj K L (1997) Biochemical changes in powdery mildew (Erysiphe polygoni D.C) resistant and susceptible cultivars of pea (Pisum sativum L.) Pl Dis Res 12: 185-88.
  10. Hiscox J D and Israelstam G F (1979) A method for extraction of chlorophyll from leaf tissues with maceration. Can J Bot 51 : 1332-34.
  11. Khan A J, Deadman M L, Srikandakumar A, Al-Maqbali Y M, Rizvi S G and Al-Sabahi J (2001) Biochemical changes in sorghum leaves infected with leaf spot pathogen Drechslera sorghicola. Plant Pathol J 17: 342-46.
  12. Kiran, Dhingra H R, Mehta N and Sangwan M S (2003) Effect of culture filtrate of Alternaria brassicae on biochemical constitutents of calli of brassicas. J Mycol Pl Path 33: 51-55.
  13. Kirk J T O and Allen R L (1965) Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem Biophys Res Commun 22: 523-30.
  14. Kolte S J and Awasthi R P (1999) Epidemiological and biochemical components of host resistance to blackspot diseases of rapeseed-mustard. 10th International Rapeseed Congress Canberra, Australia.
  15. Lowry O H, Rosenbrough N J, Farr A L and Randall R J (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 265-75.
  16. Madhavi K J, Sujatha M, Raja Ram Reddy D and Rao C (2005) Biochemical characterization of resistance against Alternaria helianthi in cultivated and wild sunflowers. Helia 28: 13-24.
  17. Malhotra S K (1993) Biochemical components of tomato genotypes in relation to fusarium wilt. Indian J Mycol Pl Pathol 23: 302-04.
  18. Pandey B R (1994) Studies of quality, seed yield and monetary return of spring okra. Veg Sci 21: 50-52.
  19. Ponmurugan P and Baby U I (2007) Morphological, physiological and biochemical changes in resistant and susceptible cultivars of tea in relation to Phomopsis disease. Plant Pathol J 6: 91-94.
  20. Prasad L and Vishunavat K (2006) Assessment of yield loss in cauliflower seed crop due to Alternaria blight. Indian Phytopath 59: 185-89.
  21. Roe J H and Oesterling M J (1943) The determination of dehydroascorbic acid and ascorbic acid in plant tissue by the 2,4 dinitrophenyl hydrazine method. J Biol Chem 152: 511-17.
  22. Satisha J, Doshi P and Adsule P G (2008) Influence of rootstocks on changing the pattern of phenolic compounds in Thompson seedless grapes and its relationship to the incidence of powdery mildew. Turk J Agric For 32: 1-9.
  23. Scarpari L M, Meinhardt L W, Mazzafera P, Pomella A M V, Schiavinato M A, Cascardo J C M and Pereira G A G (2005) Biochemical changes during the development of witches’ broom: the most important disease of cocoa in Brazil caused by Crinipellis perniciosa. J Exp Bot 56: 865-77.
  24. Senthil V, Ramasamy P, Elaiyaraja C and Ramola Elizabeth A (2010) Some phytochemical properties affected by the infection of leaf spot disease of Cucumis sativus (Linnaeus) caused by Penicillium notatum. African J of Basic & Appl Sci 2: 64-70.
  25. Siddiqua A and Kashem M A (1993) Total phenol and soluble sugar in rice leaf sheath in relation to resistance to sheath blight pathogen. Indian J Agric Res 27: 57-63.
  26. Sunkad G and Kulkarni S (2006) Studies on structural and biochemical mechanisms of resistance in groundnut to Puccinia arachidis. Indian Phytopath 59: 323-28.
  27. Swain T and Hills W E (1959) The phenolic constituents of Prunus domestica-The qualitative analysis of phenolic constituents. J Sci Fd Agric 10: 63-68.
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    volume 33 issue 2 (june 2013) : 92-97

    BIOCHEMICAL CHARACTERISTICS IMPARTING RESISTANCE AGAINST ALTERNARIA BLIGHT IN CAULIFLOWER GENOTYPES

    M
    Madhu Dhingra*
    N
    Neelima Arora
    I
    I.S. Aujla1
    1Department of Botany Punjab Agricultural University, Ludhiana- 141 004, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Dhingra* Madhu, Arora Neelima, Aujla1 I.S. (2025). BIOCHEMICAL CHARACTERISTICS IMPARTING RESISTANCE AGAINST ALTERNARIA BLIGHT IN CAULIFLOWER GENOTYPES . Agricultural Science Digest. 33(2): 92-97. doi: .

    ABSTRACT

    Alternaria blight disease has been reported from all the continents of the world, affects most of the cruciferous crops. Alternaria blight incited by foliar pathogen, Alternaria brassicae has become a major constraint in the successful cultivation of this crop. Development of resistant varieties is the most appropriate approach to control the disease and the concept is now developing to explore the built-in plant defense mechanism in relation to pathogen attack. In the present study, an attempt was made to identify biochemical characteristics imparting resistance against Alternaria blight in cauliflower genotypes. The moderately resistant genotypes had higher total chlorophyll, total phenols, total soluble sugars, total proteins, flavonoids and ascorbic acid than the susceptible genotypes. The chlorophyll content was found to be lesser in the infected leaves than the healthy ones. The reduction in total phenols, total soluble sugars, total proteins, flavonoids and ascorbic acid was at a higher rate in susceptible ones than the moderately resistant genotypes. These biochemical characteristics might be responsible for imparting resistance against Alternaria blight in cauliflower genotypes.

    REFERENCES

    1. Ammajamma R and Patil P V (2008) Biochemical factors imparting rust (Phakospora pachyrhizi) resistance in soybean. Karnataka J Agric Sci 21: 65-69.
    2. Arora N (2004) Role of phytoanticipins in resistance against red rot of sugarcane (Saccharum officinarum L.). Env & Eco 22: 861-63.
    3. Balabaa S I, Zake A Y and Elshamy A M (1974) Total flavonoid and rutin content of the different organs of Sophora japonica L. J Assoc Analyt Chem 57: 752-55.
    4. Chakrabarty P K, Mukewar P M, Raj S and Sravan Kumar V (2002) Biochemical factors governing resistance in diploid cotton against grey mildew. Indian Phytopath 55: 140-46.
    5. Chattopadhyay A K and Bagchi B N (1994) Relationship of disease severity and yield due to leaf blight of mustard and spray schedule of mancozels for higher yield. J Mycopath Res 32: 83-87.
    6. Conn K L, Tewari J P and Awasthi R P (1990) A disease assessment key for Alternaria blackspot in rapeseed and mustard. Can Pl Dis Surv 70: 19-22.
    7. Dubois M, Gilles K A, Hamilton J K, Roberts P A and Smith F (1956) Colorimetric methods for the determination of sugars and related substances. Anal Chem 28: 352-56.
    8. Ghose L, Neela F A, Chakravorty T C, Ali M R and Alam M S (2010) Incidence of leaf blight disease of mulberry plant and assessment of changes in amino acids and photosynthetic pigments of infected leaf. Plant Pathol J 95: 140-143.
    9. Guleria S, Paul B and Bajaj K L (1997) Biochemical changes in powdery mildew (Erysiphe polygoni D.C) resistant and susceptible cultivars of pea (Pisum sativum L.) Pl Dis Res 12: 185-88.
    10. Hiscox J D and Israelstam G F (1979) A method for extraction of chlorophyll from leaf tissues with maceration. Can J Bot 51 : 1332-34.
    11. Khan A J, Deadman M L, Srikandakumar A, Al-Maqbali Y M, Rizvi S G and Al-Sabahi J (2001) Biochemical changes in sorghum leaves infected with leaf spot pathogen Drechslera sorghicola. Plant Pathol J 17: 342-46.
    12. Kiran, Dhingra H R, Mehta N and Sangwan M S (2003) Effect of culture filtrate of Alternaria brassicae on biochemical constitutents of calli of brassicas. J Mycol Pl Path 33: 51-55.
    13. Kirk J T O and Allen R L (1965) Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem Biophys Res Commun 22: 523-30.
    14. Kolte S J and Awasthi R P (1999) Epidemiological and biochemical components of host resistance to blackspot diseases of rapeseed-mustard. 10th International Rapeseed Congress Canberra, Australia.
    15. Lowry O H, Rosenbrough N J, Farr A L and Randall R J (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 265-75.
    16. Madhavi K J, Sujatha M, Raja Ram Reddy D and Rao C (2005) Biochemical characterization of resistance against Alternaria helianthi in cultivated and wild sunflowers. Helia 28: 13-24.
    17. Malhotra S K (1993) Biochemical components of tomato genotypes in relation to fusarium wilt. Indian J Mycol Pl Pathol 23: 302-04.
    18. Pandey B R (1994) Studies of quality, seed yield and monetary return of spring okra. Veg Sci 21: 50-52.
    19. Ponmurugan P and Baby U I (2007) Morphological, physiological and biochemical changes in resistant and susceptible cultivars of tea in relation to Phomopsis disease. Plant Pathol J 6: 91-94.
    20. Prasad L and Vishunavat K (2006) Assessment of yield loss in cauliflower seed crop due to Alternaria blight. Indian Phytopath 59: 185-89.
    21. Roe J H and Oesterling M J (1943) The determination of dehydroascorbic acid and ascorbic acid in plant tissue by the 2,4 dinitrophenyl hydrazine method. J Biol Chem 152: 511-17.
    22. Satisha J, Doshi P and Adsule P G (2008) Influence of rootstocks on changing the pattern of phenolic compounds in Thompson seedless grapes and its relationship to the incidence of powdery mildew. Turk J Agric For 32: 1-9.
    23. Scarpari L M, Meinhardt L W, Mazzafera P, Pomella A M V, Schiavinato M A, Cascardo J C M and Pereira G A G (2005) Biochemical changes during the development of witches’ broom: the most important disease of cocoa in Brazil caused by Crinipellis perniciosa. J Exp Bot 56: 865-77.
    24. Senthil V, Ramasamy P, Elaiyaraja C and Ramola Elizabeth A (2010) Some phytochemical properties affected by the infection of leaf spot disease of Cucumis sativus (Linnaeus) caused by Penicillium notatum. African J of Basic & Appl Sci 2: 64-70.
    25. Siddiqua A and Kashem M A (1993) Total phenol and soluble sugar in rice leaf sheath in relation to resistance to sheath blight pathogen. Indian J Agric Res 27: 57-63.
    26. Sunkad G and Kulkarni S (2006) Studies on structural and biochemical mechanisms of resistance in groundnut to Puccinia arachidis. Indian Phytopath 59: 323-28.
    27. Swain T and Hills W E (1959) The phenolic constituents of Prunus domestica-The qualitative analysis of phenolic constituents. J Sci Fd Agric 10: 63-68.
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