Analysis of impact of anaerobic condition on the aflatoxin production in Aspergillus parasiticus Speare 

DOI: 10.18805/ag.A-5161    | Article Id: A-5161 | Page : 75-78
Citation :- Analysis of impact of anaerobic condition on the aflatoxin production in Aspergillus parasiticus Speare.Agricultural Science Digest.2019.(39):75-78
Maneesh Kumar, Harish Kumar, Roshan Kamal Topno and Jainendra Kumar kumar.maneesh11@gmail.com
Address : Department of Biotechnology, Magadh University, Bodh Gaya-824 234, Bihar, India.
Submitted Date : 2-11-2018
Accepted Date : 17-01-2019

Abstract

Aflatoxins are the natural carcinogens that are the best characterized as fungal secondary metabolites. The producers that are responsible for aflatoxin biosynthesis are strongly associated in toxic contamination of essential agricultural products. Aspergillus parasiticus is an exclusive fungus that participates in causing hepatic problems in humans and cattle. These mycotoxins are greatly influenced by abiotic stresses. The fungal growth, proliferation and its toxigenicity are highly influenced by these stresses. Present study aimed to restrict the mycelial growth and to prevent aflatoxin preparation in A. parasiticus under the anoxic stress. The monosporic strains of A. parasiticus were grown in two different Erlenmeyer conical flasks containing Czapek Dox Broth and Czapek Dox Agar under both aerobic and anaerobic conditions. The anoxic condition was maintained using Anaero Bag System. Aflatoxin was isolated after 10 days, and quantitative estimation was done by using High Performance Liquid Chromatography (HPLC). The experimental outcome showed that there was a drastic decrease in both the morphological growth and the aflatoxin biosynthesis of A. parasiticus in anoxic state.

Keywords

Aflatoxin Anaerobic Anaero Bag System Aspergillus HPLC.

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