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Physicochemical Alterations in Fruits Caused by Edible Coatings during Storage: A review

Pooja Ahlawat1,*, Anita Kumari1, Sarita1
1Department of Botany and Plant Physiolog, Chaudhary Charan Singh Haryana Agriculture University, Hisar-125 001, Haryana, India.

Fruits and vegetables are especially perishable due to their 80-90% water content by weight. Several strategies have been developed to retain and extend the freshness of packaged, transported and stored goods. Edible coatings are thin films applied to the exterior of fruits and vegetables to protect them from moisture, air and microbes. In the development of edible coatings, polysaccharides, proteins, lipids, composites and resins are among the most often employed materials. Packaging fresh fruits and vegetables is essential for preventing further contamination, damage and loss of moisture. The physicochemical effects of edible coatings on whole fruits include enhanced hardness, higher titratable acidity (TA) and better vitamin C, whereas the effects on fresh cuts include reduced water loss, increased soluble solid content (SSC) and preservation of product colour. The bulk of the effects might have been observed in both whole and freshly sliced fruits. This has aroused the interest of scholars, resulting in extensive research.

Fruits are nature’s wonderful gift to humanity; they are life-enhancing medications rich in vitamins, minerals, antioxidants and phytonutrients (plant-derived micronutrients). Fruits and vegetables are universally acknowledged as the primary sources of vitamins, minerals, antioxidants and fiber in consumer diets. Simultaneously, their short shelf life is widely recognized, owing to their high moisture content (75-95%), which is the primary reason of their fast disintegration. Fruits are classified into two classes based on the processes that govern the ripening process. They are classified as climacteric or non-climacteric fruits in this regard. While fruits which are non-climacteric, do not continue to ripen after harvest, fruits which are climacteric, might ripen after harvest and release more ethylene than non-climacteric fruits, making them more susceptible to deterioration caused by bacteria. As a result, adequate packaging and coating technologies are required for these food products to have a respectable shelf life (Jafarzadeh et al., 2021).
       
Perishable fruit are prone to spoilage under ambient circumstances. Post-harvest losses can be reduced by extending shelf life by monitoring transpiration, respiration, microbial infection and preserving membranes from disorganization (Muhammad et al., 2021). To some extent, the aforementioned goals can be met by using edible organic and inorganic coatings such as growth regulators, wax emulsions, potassium and calcium chemicals, irradiation, anti-transparent substances, gel, oil, lipid, starch, packaging and wrapping materials and various types of storage used as postharvest treatments. Various international and national agencies, led by the FAO, undertook studies that revealed that almost one-third of all food produced on the earth and roughly half of all fruit and vegetables are lost. To improve shelf life and marketable time to simplify long distance transportation, edible coatings of wax, oils, natural ingredients and others have been utilized as an effective technique to preserve the quality of post-harvest fruits during storage. Semi-permeable coatings have gained popularity for decreasing moisture loss, transpiration, respiration and microbial assault while preserving hardness at ambient temperatures (Fig 1).
 

Fig 1: Effects of coatings on fruits in enhancing the shelf life.


       
A huge number of novel edible materials have recently been produced; hence, the most recent studies on the issue are covered in this review. The aim of this review is to update knowledge on edible coatings on minimally processed and fresh vegetables, with an emphasis on composition, active components and their influence on physicochemical properties and shelf-life.
 
Edible coatings
 
A thin coating of edible components such as polysaccharide, protein and lipids acts as a barrier between food and the surrounding environment when applied as a thin film. These coatings prevent decay while maintaining product quality and enhance shelf life without generating anaerobiosis. They operate as moisture and gas barriers, slowing the ripening process of crops by limiting respiration rate. Edible coatings enhance functional characteristics by integrating antibacterial chemicals, antioxidants, minerals and vitamins (Manojj et al., 2021 and Hazarika et al., 2023). The components are applied to the food surface by brushing, dipping, or spraying the coating solution (Kocira et al., 2021 and Khare et al., 2017). According to the nature of the components employed in the creation of the coating solution, edible coatings are classified into three types. Alginates, proteins and polysaccharides are examples of hydrocolloids, as are lipids including acylglycerols, fatty acids, or waxes and the composites made by mixing molecules from the other two groups.
       
Hydrocolloids and lipids are commonly employed together because hydrocolloids are poor moisture barriers and lipids are poor gas barriers. The combination of both increases structural integrity and functional characteristics (Jafarzadeh et al., 2022). To increase their qualities, antimicrobial additives, antibacterial chemicals and emulsifiers can be added (Muhammad et al., 2021) (Fig 2). Edible and bio-based films are a rapidly evolving technology that provides an alternative to standard synthetic polymers, which pollute the environment severely (Fig 3). The primary benefits of ecologically friendly composites are that they are renewable and biodegradable. As a result, the creation of edible coatings has lately piqued the interest of researchers and consumers, owing to the wide range of uses provided by these materials. As Table 1 shows different combination of edible coatings used on various fruits. Those materials might be among the available food compounds that have not been investigated as edible packaging components or industrial by-products and trash that have a long-term utility.
 

Fig 2: Applications of edible films and coatings.


 

Fig 3: Benefits of edible coatings.


 

Table 1: List of fruits coated with edible coatings.


 
Application techniques for edible films and coatings
 
The technique or process by which the coating or film clings to the food surface is referred to as the application method. (Fig 4).
                              
@table4
 
I. Dipping
 
Dipping is the most frequent method for coating fruits and vegetables with very viscous coatings. The product is immersed in coating solutions for five to thirty seconds under regulated density and surface tension conditions.
 
II. Spraying
 
The spraying method is utilized for non-viscous liquids. The food product is delivered into the coating system and sprayed by regulating the ultimate drop size of the spray solution.
 
III. Brushing
 
When preventing moisture loss is a difficulty, some items, such as fresh beans and strawberries, employ the brushing approach.
 
Effect of edible coatings on physicochemical properties of fruits
 
a. Physiological loss in weight (PLW)
 
The most visible physiological change in citrus fruits after harvest is the loss of water by transpiration, which results in a loss of fruit weight and volume, as well as wilting and shriveling of the fruits. These modifications have an impact on the look of the fruits and impair their marketability. Physiological weight loss is mostly caused by evaporation and transpiration of water from fruit, as well as respiration and physiological processes that occur in fruits after harvesting. Duong et al., (2022) created an edible covering substance based on alginate and various CaCl2 solution concentrations to extend the shelf life of rose apple fruits during cold storage. In comparison to the alginate films, the addition of CaCl2 solutions reduced water vapor permeability and oxygen permeability. The coated samples had a considerably lower respiration rate and weight loss, as well as a delayed chilling damage, according to the physiochemical data. Basiak et al., (2019) investigated the effects of two distinct starch-based edible coatings on plums, one comprising simply starch and the other containing starch and whey protein. When the coatings were applied in a three-layered model, the results revealed that the starch and starch-whey protein coatings enhanced the overall resistance in the water vapor channels of individual plums by 60-75% at high transpiration potentials. Mango (Gill et al., 2015), grapes (Shiri et al., 2011) and kinnow (Ahlawat et al., 2018a) have all shown an increase in PLW during storage.
 
b. Decay loss
 
Spores of several microorganisms can be found in the atmosphere. Fruits are particularly prone to microbial contamination due to their high moisture content. Fruits have a hard surface at the solid mature stage, therefore spores from diverse microorganisms cannot penetrate the fruit surface. As fruits ripen, they soften, allowing more microorganisms to assault the fruit surface, resulting in increased decay loss. Das et al., (2020) evaluated the antibacterial and antibiofilm properties of newly developed edible nano-emulsion coatings utilizing sodium alginate and essential oil derived from sweet orange. Salmonella and Listeria were chosen as pathogenic bacterial strains for this investigation. The edible nano-emulsion coatings did not allow bacterial growth of the two tested microorganisms. In terms of the quality attributes of the coated tomato samples, it was discovered that firmness was significantly improved, up to 33% and weight loss was reduced up to 3-fold compared to uncoated samples. Gangwar et al., (2012) found that decay loss varied with storage duration (0-15 days) in different Aonla (Emblica officinalis) or Indian gooseberry cultivars maintained at room temperature. After 15 days of storage, the fruits of Aonla cultiver NA-7 had the lowest decay loss (15.12%), whereas the fruits of cultivar Banarasi had the highest decay loss (19.33%).
 
c. Firmness
 
The degree and amount of firmness loss during storage is one of the key parameters used to predict fruit quality and post-harvest shelf life. Fruit softening is caused by a biochemical process that involves the breakdown of pectin and starch by enzymes such as wall hydrolases. Edible coatings and packaging materials enhanced fruit firmness because coatings limit water loss. Oyom et al., (2022) used an edible coating formulation incorporating modified starch from sweet potatoes and cumin essential oil on pear samples in research (Pyrus bretchneideri Rehd). The coating method employed in the testing was dipping the sterilized samples into the coating solutions and the coated samples were kept at 25°C for 28 days. Because of the adhesiveness of the modified starch coating, which created a thin layer on the pear and kept the pericarp and freshness, the coating treatment was able to control the respiration rate, postpone weight loss and maintain flesh firmness after 21 days. According to Bishnoi et al., (2014), there was no significant difference in hardness between uncoated and coated plums after 8 days of storage at ambient temperatures, while coated plums remained much firmer even after 16 days of storage.
 
d. Total soluble solids (TSS)
 
A total soluble solid defines the quality of fruits, which include 90% sugars and 10% minerals and acids. TSS increases during storage due to starch breakdown into soluble sugars. According to Ali et al., (2010), there was a progressive rise in soluble solid content over the whole storage time of tomato fruits, which was 20 days. The soluble solid level was much greater in control fruit (10.1%) than in coated fruit, with the lowest soluble solid concentration (6.5%) reported in fruit coated with 20% gum arabic, indicating that the coatings created a good semi permeable membrane surrounding the fruit. According to Mohammed et al., (2014), chitosan coating treatment delayed the decline in total soluble solids concentrations from 19.5% on the first day of storage to 17.8% on the 60th day of storage when compared to control fruits (13.7%). Din et al., (2015) investigated the influence of edible coatings, such as black cumin oil, bitter mustard oil and mustard oil, on kinnow fruit preservation for up to 30 days. The TSS of all treatments ranged from 12.43 to 12.52°brix. Khaliq et al., (2016) discovered that chitosan 1% and gum arabic 10% coating treatments effectively prevented the growth of TSS throughout a 28-day storage period.
 
e. Specific gravity
 
Due to higher transpiration and respiration during storage, fruits lose more weight than volume during storage. As a result, specific gravity decreased as the storage duration lengthened. Singh et al., (2012) documented a decrease in specific gravity during storage in mango. Kumari (2016) determined that the specific gravity of Aonla fruits fell from 1.11 to 0.91 after 15 days of storage at room temperature. Singh et al., (2012) discovered a reduction in specific gravity as the storage time (0-10 days) progressed in Amrapali Mango. Amrapali Mango had a maximum specific gravity of 0.99 on the first day of storage and a minimum specific gravity of 0.79 on the tenth day of storage. Fruit specific gravity increases as the maturity index progresses and fruit ripeness at harvest impacts its quality and storage life.
 
f. Juice content
 
The juice content reduces with storage time. Fruit weight was closely connected to a decrease in liquid percent. Increased evapotranspiration and catabolic processes in the fruits might explain the declining trends in juice content. Kaur and Kumar (2014) evaluated the impact of mandarin fruits treated with CaNO3 (0.5% and 1.0%), CaCl2 (1% and 2%) for 45 days of storage and found that CaNO3 (1%) treated fruits had the highest juice percentage, 43.53%, when compared to control fruits (39.56%). Bhatnagar et al., (2015) found that the juice recovery percentage of kinnow fruits varied from 41.11% (at the Chattargarh 1 orchard) to 51.39% (at the Khajuwala 1 orchard). Ahlawat et al., (2018c) investigated the effect of Gum Arabic edible coating on Kinnow juice content. On a mean basis, regardless of storage length, fruits coated with gum arabic 10 per cent (27.20%) had the highest juice content, followed by fruits coated with gum arabic 5 per cent (27.18%).
 
g. Acidity
 
Citrus organic acids, particularly citric acids, serve as a reserve source of energy. The acidity of fruits can change depending on ripeness and storage temperature. This is clear because acids may have been used in various physiological processes (for example, respiration) and may have remained on the fruits throughout storage. This might possibly be attributed to an increase in the activity of the enzyme invertase, which is responsible of converting acids into sugars. Muley et al., (2020) evaluated the effects of a new functional coating comprised of whey protein isolate, chitosan and glycerol on the shelf-life extension of strawberries. The films were cast at temperatures above 60°C and many physical studies were carried out (antioxidant activity, color, oxygen transfer rate, crystallinity, etc.). Fresh strawberry samples were covered with these films and stored for eight days at two different temperatures (5°C and 20°C). The samples were subjected to a range of biochemical and physical examinations in order to determine their shelf-life. The coated samples displayed lower titratable acidity, weight loss, pH, color changes, total phenolics and DPPH levels. In terms of shelf-life extension, the untreated samples had a shelf life of 3 to 5 days while the coated samples had a shelf life of 5 to 8 days. According to Ali et al., (2015), fruit acidity decreased with increasing storage period for all treatments up to 63 days, but the decrease in acidity was slightly less for coated kinnow mandarins (from 1.01% to 0.81% for natural fruit coating and from 1.03% to 0.82% for Fomesa) than for uncoated ones (from 1.02% to 0.79%). Grapes (Shiri et al., 2011), Kinnow (Ahlawat et al., 2018a) and Aonla (Kumari, 2016) all have shown a decrease in acidity during storage. It has been found that many fruits treated with edible coatings and films retain titratable acidity.
 
h. Ascorbic acid
 
Ascorbic acid is essential in human nutrition. When exposed to oxygen, ascorbic acid is extremely reactive and easily oxidized. The drop in ascorbic acid concentration in fruits during storage might be attributed to ascorbic acid oxidase enzyme converting ascorbic acid to dehydro-ascorbic acid. Temperature and storage time have been shown to influence the ascorbic acid concentration. Paladugu and Gunasekaran (2017) investigated the effect of gum arabic used as an edible covering on tomatoes preserved for 28 days. The highest ascorbic acid level was found in control fruit (27 mg/100 g), while the lowest was found in fruit coated with 1.5% gum arabic (14 mg/100 mg). Javed et al., (2015) measured ascorbic acid content in guava fruits treated with 0, 1, 2 and 3% calcium lactate for up to 24 days. The lowest ascorbic acid content was found in fruits treated with 0% calcium lactate (318 mg/100 g) and the highest was found in fruits treated with 3% calcium lactate (337 mg/100 g). This was owing to the coating’s low oxygen permeability, which delayed the deteriorative oxidation process of the ascorbic acid content. According to Ali et al., (2015), the highest ascorbic acid content was found in kinnow mandarins coated with Fomesa (26.06%), followed by those coated with natural fruit coating (26.05%) and the lowest ascorbic acid content was found in uncoated kinnow mandarins (24.77%) after 20 days of storage. These findings are consistent with prior research, which discovered that the ascorbic acid concentration of waxed and unwaxed kinnow fruits declined during low-temperature storage and that coated fruits had greater ascorbic acid amounts than uncoated ones (Mahajan et al., 2013).
 
i. Sugars
 
The amount of sugar in fruit impacts its sweetness and flavor. TSS is closely connected to changes in sugar concentration during storage. The conversion of starch and polysaccharides into soluble sugars, as well as fruit drying, are likely to cause a rise in sugars during storage. Javed et al., (2015) investigated the effect of various calcium lactate concentrations (0, 1%, 2% and 3%) on guava fruits stored for up to 24 days. Sugar content was highest in control fruits (8.88 g/100 g) and lowest in fruits treated with 3% calcium lactate (9.94 g/100 g). According to Athmaselvi et al., (2013), aloe vera coated tomatoes had a greater sugar content (4.31 mg/100 g) than uncoated tomatoes (3.49 mg/100 g). According to Pawar et al., (2011), the total and reducing sugar content of sapota fruit rose considerably after storage, rising from 14.40% and 8.90% to 19.12% and 11.08%, respectively. Catharanthus reducing sugars surged first and then progressively reduced, whereas non-reducing sugars decreased initially and eventually grew.
 
j. Enzymatic studies
 
a) Pectin methylesterase (PME), polygalacturonase (PG) and cellulase
 
Softening changes, including a loss of brittleness and stiffness, are the most prominent indicators of kinnow degradation; these changes have a direct impact on the quality of the fruits as well as their storage life. The action of enzymes such as pectin methylesterase (PME), polygalacturonase (PG) and cellulase on polysaccharides such as pectin, hemicellulose and cellulose present in the cell wall has been related to the disassembly and destruction of cell wall structure and composition. Pectin methylesterase hydrolyzes pectins, which are the principal elements of the fruit’s middle lamella and primary cell wall, in higher plants to produce demethylated pectins that are more readily hydrolyzed by polygalacturonase.
       
Furthermore, polygalacturonase catalyze the hydrolysis of (1®4) galacturonan linkages in demethylated pectins, resulting in shorter chains and the depolymerization and dissolution of pectins. Vishwasrao and Ananthanarayan (2017) investigated the effect of treating sapota fruits (a big berry) with an edible covering made of methyl cellulose (MC) and palm oil (PO) on shelf life. During postharvest ripening, the examined fruit samples demonstrated lower pectin methylesterase activity. Overall, the edible coating consisting of methyl cellulose (MC) and palm oil (PO) preserved the treated sapota fruit samples, prolonging the shelf life by three days at 24.1°C and 65.5% RH compared to the control samples. Ahlawat et al., (2018d) studied kinnow fruits coated with varying concentrations of gum arabic, calcium lactate and glycerin and found that their combination resulted in a progressive increase in PG activity during storage, but the increase was much greater in the control throughout the entire storage period. Glycerin 2.5 per cent treated fruits (0.125 units/g FW) had the highest PG activity (0.127 units/g FW). Gum Arabic 10% + Glycerin 2.5% coated fruits had the lowest PG activity, 0.120 units/g FW. The reduced activity of polygalacturonase in coated fruits might be attributed to the coating successfully preventing access of pectinolytic enzymes to the cell wall substrate and so helping to retain fruit firmness.
 
b) Free radical scavenging activity (DPPH)
 
The DPPH (1,1-Diphenyl-2-Picryhydrazyl radicle) is widely acknowledged as a stable free radical and DPPH radical-scavenging by antioxidants has been linked to their hydrogen donating capacity. Wani et al., (2021) investigated the edible coatings of Arabic gum, xanthan gum with 1% w/v lemon grass essential oil and carrageenan. The results revealed that the coated strawberry samples reduced weight loss, better retimed ascorbic acid, had higher antioxidant activity and increased firmness. The anthocyanins levels and phenolic compounds were retained in the edible films containing carrageenan gum after storage. Furthermore, the coatings containing carrageenan gum produced the greatest results in terms of quality preservation during storage.
       
Khaliq et al., (2016) investigated the effects of 10% gum arabic, 3% calcium chloride and their combination on mango over 28 days. Based on the results collected, they determined that the DPPH radical scavenging activity of fruit treated with gum arabic 10% and calcium chloride 3% + gum arabic 10% was substantially greater (60%) than in the control fruit 28%. Javed et al., (2015) measured antioxidant activity in guava fruits that had been dipped in 1, 2 and 3% calcium lactate solutions for 5 minutes and kept for up to 12 days. The greatest decrease in antioxidant activity was observed in the control group, which varied from 34.00% to 24.67% and 15.67% from 0 to the 6th and 12th days, respectively and for calcium lactate 1% and calcium lactate 2%, the values varied from 34.33% to 26.33% and 34.33% to 29.33% from 0 to the 6th day, respectively.
Recent research on newly developed coverings for fresh and minimally processed fruits and vegetables is presented in this article. Recent developments in this field include the widespread use of chitosan or alginate as the principal ingredient in edible coatings. A fuller understanding of the mechanism of edible functional coatings, as well as consumer marketing, could assist in expanding their fruit and vegetable preservation applications. In addition, edible coatings and films have the potential to be a very appealing method for delivering bioactive compounds to enhance bioavailability. Due to the very specific coating technology applied to different fruits and/or vegetables, with various proposed goals (ranging from extending shelf life to preserving a high nutritional value or enhancing certain nutritional characteristics of the products), this topic is perennially relevant and experiments must be conducted beginning with known data.
All authors declare that they have no conflict of interest.

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