Sex preselection by separating the male- and female-bearing spermatozoa is vital for efficient animal production. In the past, various techniques have been evolved with paucity of scientific validation. Among them, Flow cytometry, which is based on the principle of difference in the DNA content, is the best and efficient method. Efforts are in place to exploit this technique in animals. In the last decade, high-speed sperm sorting with the orienting nozzle resulted in production of X- and Y-sperm with 90% accuracy at the rate of 2-6 million sperm per hour. This application can enable to accomplish more conventional technology for both artificial insemination and cryopreservation of X- or Y-sperm in farm animals. Recently, the process of commercialization of sexed sperm hastened. However, this technology has some impediment with respect to cost of production, implementation and pregnancy rate than control sperm. Despite these limitations, production of sexed semen usually followed by cryopreservation is being used commercially for cattle and horse production.