Molecular techniques in rumen biotechnology: A review

DOI: 10.18805/ar.v37i1.9265    | Article Id: R-1544 | Page : 55-60
Citation :- Molecular techniques in rumen biotechnology: A review .Agricultural Reviews.2016.(37):55-60

Manju G. Preedaa*1, Thulasiraman Parkunan2, Dhinesh Kumar R.2, Ramavathi J.,  Yazhini P., Aasif Ahmad Sheikh2, Mohammad Rayees Dar2, Lakshmi Priyadarshini2, Gunjan Baghel2 and Chandrasekar T.2

preprand@gmail.com
Address :

Veterinary College and Research Institute, Namakkal -637 002, India.

Submitted Date : 9-06-2015
Accepted Date : 28-01-2016

Abstract

This review analyses the use of molecular techniques in rumen microbial identification. The use of regular methods like roll tube and most probable number resulted in under estimation of rumen microbial growth. So molecular biology acts as an advanced tool for rumen microbial culture and identification of various new species. These techniques give complete descriptions of individual ruminal populations. Use of molecular techniques like PCR, DDGE and FISH, which also pave pathway for genetic manipulation of rumen microbes in the field of rumen manipulation. So the combination of traditional and molecular assays gives accurate and satisfactory results.

Keywords

DNA Microbes Molecular methods PCR Rumen RNA.

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