Agricultural Reviews

  • Chief EditorPradeep K. Sharma

  • Print ISSN 0253-1496

  • Online ISSN 0976-0741

  • NAAS Rating 4.84

Frequency :
Quarterly (March, June, September & December)
Indexing Services :
AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Agricultural Reviews, volume 34 issue 4 (december 2013) : 279-287

IN VITRO SHOOT TIP GRAFTING IN CITRUS SPECIES- A REVIEW

Lal Chand, Suneel Sharma*, Rajpal Dalal, Anil K. Poonia1
1Department of Horticulture CCS Haryana Agricultural University, Hisar-125 004, India
Cite article:- Chand Lal, Sharma* Suneel, Dalal Rajpal, Poonia1 K. Anil (2024). IN VITRO SHOOT TIP GRAFTING IN CITRUS SPECIES- A REVIEW. Agricultural Reviews. 34(4): 279-287. doi: 10.5958/j.0976-0741.34.4.013.

ABSTRACT

A retrospective overview of work done on the in vitro shoot tip grafting in citrus in the world was taken up. In vitro shoot tip grafting is the most reliable method to recover free citrus saplings from infected parental source. The technique of in vitro shoot tip grafting is a suitable method for the elimination of graft transmissible diseases in citrus. Preliminary  studies  showed  that  certain  pathogens  are  difficult  to be  eliminate from  mother  plant  like  citrus  exocortis  and  stubborn, which  might  be  eliminated  by  a  process  of  shoot  tip  grafting  in-vitro. Plant  obtained  by  micro-grafting  do  not  have the  same  problems  as  nucellar  plants  such  as  reversion  to  the  juvenile  state,  excessive  thorniness,  vigorous  and  upright  habit  of  growth,  slowness  to  fruiting,  alternate  bearing  in  early  years and physical  differences  in  fruit  characteristics.

REFERENCES

  1. Ali, H.M.A.; Osman, M. Elamin and Mohamed A. Ali. (2005) Propagation of grapefruit by shoot tip micrografting. Proceedings of the 37th Meetings of the National Crop Husbandry Committee, Sudan. pp. 220-227
  2. Ali, A.; Naz, Muhammad, J.J.; Haider, A. and Qasim, M. (2007) In-vitro studies on micrografting technique in two cultivars of Citrus to produce virus free plants. Pakistan Journal of Botany, 39(5): 1773-1778.
  3. Burger, D.W. (1984) Micrografting- A tool for the plant propagator. Proceedings of the International Plant Propagator’s Society, 34: 244-248.
  4. Chatisathion, J. and Tontyaporn, S. (1990) In vitro culture for the production of disease-free citrus in Thailand. In: Aubert, B., Tontyaporn, S. and Buangsuwon (ed) Proceedings of the 4th International Asia Pacific Conference on Citrus Rehabilitation, pp. 69-75. Chaing Mai, Thailand.
  5. Chaturvedi, H.C. and Sharma, A.K. (1988) Citrus tissue culture. Proceedings of the National Seminar on Plant Tissue Culture, pp. 36-46, New Delhi.
  6. Chen, R. Z.G.G. and Zhang, L.Y. (1992). Study on in vitro grafting and micro-propagation of triploid citrus plantlets. China Citrus, 21: 10-12.
  7. Dass, H.C.; Vijakumari, N. and Singh, A. (1997) In vitro shoot tip grafting in Nagpur Mandarin. Indian Horticulture, 42: 28-29.
  8. Dass, H.C., Vijayakumari, N. and Singh, A. (1995) Biotechnological research for improvement of Nagpur Mandarin. NRCC, Annual Report, pp. 7-8.
  9. Debergh, P.C. (1991) Acclimatization techniques of plants from in vitro. Acta Horticulturae, 289: 291-299.
  10. De-Lange, J.H.; Van-Vuuren, S.P.and Bredell, G.S. (1981) Groeipunt-enting suiwer sitrusklone vir die superplantskema van virusse. Subtropica, 2(5): 11-16.
  11. Edriss, M.H. and Burger, D.W. (1984) Micrografting shoot tip culture of citrus on three trifoliate rootstocks. Scientia Horticulture, 23: 255-59.
  12. Fourie, C. (1988) Success with growing-point grafting for citrus. Information Bulletin of the Citrus and Subtropical Fruit Research Institute, 182: 1-2.
  13. Fourie, C.J. (1991) Improved shoot tip grafting of citrus. Inligtingsbulletin, Navorsingsinstituut vir Sitrus en Subtropiese Vrugte, 223: 10-12.
  14. Ghorbel, B.R.; Navarro, L. and Duran-Vila, N. (1998) Morphogenesis and regeneration of whole plants of grapefruit (Citrus paradisi), Sour orange (C. aurantium) and alemow (C. macrophylla). Journal of Horticultural Science and Biotechnology, 323-27.
  15. Grewal, H.S.; Dhat, A.S. and Gosal, S.S. (1994) In vitro shoot-tip grafting in citrus. Annals of Biology (Ludhiana), 10:1-6.
  16. Hassanein, A.M. and Azooz, M.M. (2003) Propagation of Citrus reticulata via in vitro seed germination and shoot cuttings. Biologia Plantarum, 47(2): 173-177.
  17. Hollings, M. (1965) Disease control through virus-free stock. Annual Review of Phytopathology, 3: 367-396.
  18. Hosoi, T.; Machida, H.; Ooishi, A. and Miura, T. (1979) Studies on shoot tip grafting of citrus. Bulletin of the Faculty of Agriculture, Shizuoka University, 29: 11-17.
  19. Huang, L.C.; Chen, W.L. and Chiu, D.S. (1988) In vitro graft-enhanced nuclear plant development in the monoembryonic Citrus grandis L. Journal of Horticultural Science, 63: 705-709.
  20. Jonard, R. (1986) Micro-grafting and its application to tree improvement. In: Bajaj Y.P.S. (ed) Biotechnology in Agriculture and Forestry, 1: 31-48. Springer. Berlin Heidelberg, New York.
  21. Jonard, R.; Hugard, J.; Macheix, J.J.; Martinez, J.; Mosella-chancel, L.; Poessel, J.L. and Villemur, P. (1983) In vitro micro-grafting and its applications to fruit science. Scientia Horticulturae, 20: 147-159.
  22. Jonard, R.; Lukman, D.; Chimot-Schall, F. and Villemur P. (1990) Early testing of graft-incompatibilities in apricot and lemon trees using in vitro techniques. Scientia Horticulturae, 43: 127–128.
  23. Juarez, J.; Molins, M.I.; Navarro, L. and Duran-Vila, N. (1990) Separation of citrus viroids by shoot-tip grafting in vitro. Plant Pathology, 39: 472-476.
  24. Karunakaran, G.H.; Ravishankar, D.K.; Samuel and Sathiya Priya, N. (2006) Standardization of shoot tip grafting in Coorg mandarin (Citrus reticulata Blanco). Proceeding of the National Symposium on Citriculture, NRCC, Nagpur, pp. 1-4.
  25. Koizumi, M. (1984) Elimination of tatter leaf-citrange stunt virus from Satsuma mandarin by shoot tip grafting following pre-heat treatment. In: Garnsey, S.M., Timmer, L.W. and Dodds, J.A. (ed.) Proceedings of the 9th conference of the international organization of citrus virologists (IOCV), Riverside, USA, pp. 229-233.
  26. Kumar, B.; Mistry, N.C.; Singh, B. and Gandhi, C.P. (2011) Indian horticulture data base. National Horticulture Board, Gurgaon, Haryana.
  27. Kumar, Raj (2009) Standardization of micropropagation techniques in Kinnow mandarin (Citrus nobilis x C. deliciosa). Thesis submitted to SKRAU, Bikaner.
  28. Lee, K.; Wu, L.; Huang, J. and Kuo, C. (1981) A preliminary study on the shoot tip apex culture of Citrus sinensis. In: Han, H. (ed.) Plant Tissue Culture. pp. 527. Pitman Advanced Publishing Program, Boston.
  29. Loannou, M.; Kyriakou, A. and loannou, N. (1991) Production of healthy Lapithos lemon plants by shoot tip grafting in vitro. Technical Bulletin-Cyprus Agricultural Research Institute, 139:7.
  30. Mishra, M. and Yadav, D.S. (1999) Effect of antioxidants on success of micro-grafting. International Symposium on Citriculture, Nov. 23-27, NRCC, Nagpur, India (Abstract) pp 24.
  31. Morel, G and Martin, C. (1952) Guerison de Dahlias attaints d’ une maladie a virus. C. R. Acad. Sci., Paris, 235: 1324- 25.
  32. Mukhopadhyay, S.; Raj, J.; Sharma, B.C.; Gurung, A.; Sengupta, R.K. and Nath, P.S. (1997) Micropropagaion of Darjeeling orange (Citrus reticulata Blanco) by shoot-tip grafting. Journal of Horticultural Science, 72(3): 493499.
  33. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  34. Murashige, T.; Bitters, W.P.; Rangan, T.S.; Nauer, E.M.; Roistacher, C.N. and Holliday, P.B. (1972) A Technique of shoot apex grafting and its utilization towards recovering virus free Citrus clones. HortScience, 7(2): 118-119.
  35. Nauer, E.M.; Roistacher, C.N.; Carson, T.L. and Murashigue, T. (1983) In vitro shoot tip grafting to eliminate citrus viruses and virus-like pathogens produces uniform budlines. Hortscience, 18: 308-309.
  36. Navarro, L. (1981) Shoot-tip grafting in vitro (STG) and its application: A review. Proceeding of the International Society of Citriculture, 1: 452-456.
  37. Navarro, L. (1984) Citrus tissue culture. In: Micropropagation of selected root crops, palms, citrus and ornamental plants (plant protection and production, Paper 59). FAO Rome, pp. 113-154
  38. Navarro, L. (1988) Application of shoot tip grafting in vitro to woody species. Acta Horticulturae, 227: 43-55
  39. Navarro, L. (1990) Shoot-tip grafting in vitro of woody species and its influences on plant age. In : Rodrigueg R, Sanchez-Tames R and Durzan D J (ed) Plant Aging: Basic and Applied Approaches. pp. 117-23. Plenum Press, New York, USA.
  40. Navarro, L. (1992) Citrus shoot tip grafting in vitro. In: Bajaj Y P S (ed.) Biotechnology in Agriculture and Forestry, 18: 327–38. Springer-Verlag, Berlin.
  41. Navarro, L.; Ballester, J.F.; Juarez, J.; Pina, J.A.; Arregui, J.M. and Bono, R., Fernandez, de, Cordova, L. and Ortega, C. (1980a) The citrus variety improvement programs in Spain (CVIPS) after four years. In: Proceeding of 8th Conference of International Orgaanization of Citrus Virologists (IOCV), Riverside, USA, pp. 289-294.
  42. Navarro, L.; Juarez, J.; Ballester, J.F. and Pina, J.A. (1980b) Elimination of some citrus pathogens producing psorosis- like leaf symptoms by shoot tip grafting in vitro. In: Proceedings of the 8th Conference of the International Organization of Citrus Virologists (IOCV), Riverside, USA, pp. 162-166
  43. Navarro, L.; Juarez, J.; Pina, J.A. and Ballester, J.F. (1984) The citrus quarantine station in Spain. In: Garnsey S M, Timmer L W and Dodds J A (ed) Proceedings of the 7th conference of the International Organization of Citrus Virologists (IOCV), Riverside, USA, pp. 365-70.
  44. Navarro, L.; Juarez, J.; Pina, J.A.; Ballester, J.F. and Arregui, J.M. (1988) The citrus variety improvement program in Spain after 11 years. In Timmer L W, Garnsey S M and Navarro L (ed) Proceedings of the 7th Conference of the International Organization of Citrus Virologists (IOCV), Riverside, USA, l: 400-406.
  45. Navarro, L.; Llacer, G.; Cambera, M.; Arregui, J.M., and Juarez J. (1982) Shoot tip grafting in vitro for elimination of viruses in peach plants (Prunus persica Batsch). Acta Horticulturae, 130:185-192
  46. Navarro, L.; Roistacher, C.N. and Murashige, T. (1975) Improvement of shoot tip grafting in-vitro for virus free Citrus. Journal of the American Society for Horticultural Science, 100(5): 471-479.
  47. Navarro, L.; Roistacher, C.N., and Murashige, T. (1976) Effect of size and source of shoot tips on Psorosis A and exocortis of navel orange plants obtained by shoot tip grafting in vitro. In: Timmer L W, Garnsey S M and Navarro L (ed) Proceedings of the 7th conference of the International Organization of Citrus Virologists (IOCV), Riverside, pp. 194-197.
  48. Palma, B.; Bravo, H. and Castro, M. (1997) Comparative study of root systems in Citrus macrophylla seedlings and microcuttings propagated in vitro. Acta Horticulturae, 447: 591-96.
  49. Parthasarathy, V.A.; Nagarju, V. and Rahman, S.A.S. (1997) In vitro grafting of Citrus reticulata Blanco. Folia Horticulturae, 9: 87-90.
  50. Pierik, R.L.M. (1988) Handicaps for the large scale commercial application of micropropagation. Acta Horticulturae, 230: 63-71.
  51. Plastira, V. (1987) Application of the method of micrografting to the mandarin cultivar Clementine Poros to obtain virus- free plants. Annales de I ‘Instituute Phytopathologique Benaki, 15: 95-108.
  52. Ramsunder, V.; Sathiamoorthy, S. and Thamburaj, S. (1999) Shoot tip grafting in vitro in acid lime (Citrus aurantifolia (Christm) Swingle). International Symposium on Citriculture, Nov. 23-27. NRCC, Nagpur, India (Abstract) pp. 21.
  53. Roistacher, C.N. and Kitto, S.L. (1977) Elimination of additional citrus viruses by shoot tip grafting in vitro. Plant Disease Reporter, 61: 594-96.
  54. Roistacher, C.N.; Navarro, L. and Murashige, T. (1976) Recovery of citrus selections free of several viruses, exocortis viroid and Spiroplasma citri by shoot-tip grafting in vitro, In: Calavan E C (ed.) proc 7th Conf Int Orgn Citrus Virol. pp. 186-93, IOCV, Riverside, USA.
  55. Russo, F. and Starrantino, A. (1973) Ricershe Sulla tecnologia dei microinnesti nel quadro del milioramento genetico- sanitario degli agrumi. Annali 1st. Sper. Agrum. Acireale., 6:209-222.
  56. Savita, Vijay.; Virk, G.S. and Nagpal, A. (2010) Effect of explant type and different plant growth regulators on callus induction and plantlet regeneration in Citrus jambhiri Lush. Environment and We: An International Journal Science and Technology, 5: 97-106.
  57. Shinde, Ekta D. and Jogdande, N.D. (2010) Effect of different method of placement of shoot tips on in vitro shoot tip grafting in Nagpur mandarin. Indian J. Hort. 67(Special Issue), November 2010: 26-28
  58. Singh, B.;Sharma, S.; Rani, G.; Hallan, V.; Zaidi, A.A.;Virk, G.S. and Nagpal, A. (2008) In vitro micrografting for production of Indian citrus ring spot virus (ICRSV) free plants of Kinnow mandarin (Citrus nobilis Lour. X C. deliciosa Tanore). Plant Biotechnology Reports, 2:137-143.
  59. Singh, S.K.; Khawale, R.N. and Singh, S.P. (2003) A comparative studies on success of in vitro shoot tip grafting in Citrus reticulata Blanco on different rootstocks. Haryana Journal of horticultural Science, 32 (3,4):171-173.
  60. Singh, S.K.; Sharma, H.C.; Singh, S.P., and Sharma, R.R. (1999) A comparative study of success of in vitro micrografting in Citrus reticulata on different rootstock. International Symposium on Citriculture, Nov. 23-27. NRCC, Nagpur, India (Abstract) pp. 20.
  61. Starrantino, A. (1991) In vitro micrografting of citrus, Petria, 2: 27-35.
  62. Starrantino, A. and Caruso, A. (1988) The shoot tip grafting technique applied in citriculture. Acta Horticulturae, 227: 101-103.
  63. Tusa, N. and Geraci, G. (1988) In vitro propagation of some citrus rootstocks. Acta Horticulturae, 227: 464-466.
  64. Tusa, N.; De-Pasquale, F. and Radogna, L. (1978) Research on the micrografting technology in Citrus. Proceedings of the International Society of Citriculture, 1: 143-145.
  65. Vijayakumari, N. and Singh, S. (1999a) In-vitro shoot tip grafting of Citrus exotic germplasm. Proceedings of the International Symposium on Citriculture, Nov. 23- 27. NRCC, Nagpur, India (Abstract) pp. 23.
  66. Vijayakumari, N. and Singh, S. (1999b) Effect of size, source of shoot tip and growth regulators on success of in vitro shoot tip grafting in Citrus reticulate Blanco cv. Nagpur mandarin. Procee-dings of the International Symposium on Citriculture, NRCC, Nagpur, pp. 245-248.
  67. Vijayakumari, N. and Singh, S. (2006) “Shoot Tip Grafting” a alone option for a sound and sustainable citrus industry. Proceedings of National Symposium on Citriculture, NRC, Nagpur. pp 53-56.
  68. Wilkins, C.P.;Cabrera, J.L. and Dodds, J.H. (1985) Tissue culture propagation of tree, Outlook on Agricuture, 14: 2-13.
  69. Youtsey, C.O. (1978) A method for virus-free propagation of citrus: Shoot tip grafting. Citrus Industry, 59: 39-47.
  70. Zarei, A. and Rahimian, H. (1997) Elimination of citrus tristeza virus from two cultivars of Satsuma mandarin through shoot-tip grafting. Iranian Journal of Plant Pathology, 33: 28-30.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)