Agricultural Reviews

  • Chief EditorPradeep K. Sharma

  • Print ISSN 0253-1496

  • Online ISSN 0976-0741

  • NAAS Rating 4.84

Frequency :
Quarterly (March, June, September & December)
Indexing Services :
AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Agricultural Reviews, volume 33 issue 2 (june 2012) : 150-158


Nitin E. Gade*, Amar Nath, M.D. Pratheesh, P. K. Dubey, Amarpal, G. Sai Kumar, G. Taru Sharma1
1Physiology and Climatology Division, IndianVeterinary Research Institute, Izatnagar- 243122, India
  • Submitted|

  • First Online |

  • doi

Cite article:- Gade* E. Nitin, Nath Amar, Pratheesh M.D., Dubey K. P., Amarpal, Kumar Sai G., Sharma1 Taru G. (2024). STEM CELL THERAPY IN ANIMAL SCIENCES – A REVIEW. Agricultural Reviews. 33(2): 150-158. doi: .
Stem cells are undifferentiated cells of body having tremendous potential to self-renew and develop into different cell types to carry out various functions. These distinctive properties make their use dynamic in regenerative therapy. Stem cell research in animal sciences during earlier decades dealt with standardizing protocols for isolation and culture along with evaluation of stem cell specific markers for their characterization. Embryonic stem cells (ESCs) are more commonly used for cloning, drug discovery, gene targeting, transgenic production. In experimental animal models, ESCs are utilized in therapeutics after in vitro directed differentiation to desired cell type. Adult stem cells are more commonly used in therapeutics as compared to ESCs due to certain practical reasons and features such as ease of isolation and expansion, their multipotency and low immunogenicity. Adult stem cells have been used in animal models for treatment of spinal injuries, tendon, cartilage and ligament defects, wounds and bone defects. In near future, stem cell therapy will provide a sensible approach to various chronic, unresponsive clinical cases and neurological disorders in veterinary field. In this report, an overview of the application of stem cell therapy for treatment of various animal diseases with regard to experimental and clinical cases has been summarized.
  1. Adel, N. and Gabr, H. (2007). Stem cell therapy of acute spinal cord injury in dogs. Third World Cong. Regen. Med., 2(5):523.
  2. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thomson, J.A. (2000). Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol., 227:271-278.
  3. Awad, H., Butler, D., Boivin, G., Smith, F.N., Malaviya, P., Huibregtse, B. and Caplan, A.I. (1999). Autologous mesenchymal stem cell-mediated repair of tendon. Tissue Eng., 5:267-277.
  4. Azari, O., Babaei, H., Derakhshanfar, A., Nematollahi-Mahani, S., Poursahebi, R. and Moshrefi, M. (2011). Effects of transplanted mesenchymal stem cells isolated from Wharton’s jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation. Vet. Res. Comm., 35(4):211-222(12).
  5. Bach, F.H., Albertini, R.J., Joo, P., Anderson, J.L. and Bortin, M.M. (1968). Marquette Bone-marrow transplantation in a patient with the Wiskott–Aldrich syndrome. Lancet, 2:1364-1366.
  6. Behboodi, E., Bondareva, A., Begin, I., Rao, K., Neveu, N., Pierson, J.T., Wylie, C., Piero, F.D., Huang, Y.J., Zeng, W., Tanco, V.,Baldassarre, H., Karatzas, C.N. and Dobrinski, I. (2011). Establishment of goat embryonic stem cells from in vivo produced blastocyst-stage embryos. Mol. Reprod.Develop., 78(3):202-211.
  7. Beltrami, A.P., Urbanek, K. and Kajstura, J. (2001). Evidence that human cardiac myocytes divide after myocardial infarction. N. Engl. J. Med., 344: 1750-1757.
  8. Ben-Hur, T., Idelson, M., Khaner, H., Pera, M., Reinhartz, E., Itzik, A. and Reubinoff, B.E. (2004). Transplantation of human embryonic stem cell-derived neural progenitors improves behavioral deûcit in parkinsonian rats. Stem Cells, 22:1246-1255.
  9. Bi, Y., Ehirchiou, D., Kilts, T.M., Inkson, C.A., Embree,M.C., Sonoyama,W., Li, L., Leet, A.I., Seo, B.M., and Zhang, L., (2007). Identiûcation of tendon stem/progenitor cells and the role of the extracellular matrix in their niche. Nat. Med., 13:1219–1227.
  10. Bieback, K., and Kluter, H. (2007).Mesenchymal stromal cells from umbilical cord blood. Curr. Stem Cell Res. Ther., 2:310-323.
  11. Borena, B.M., Pawde, A.M., Amarpal, Aithal, H.P., Kinjavdekar, P., Singh, R. and Kumar, D. (2009).Autologous bone marrow-derived cells for healing excisional dermal wounds of rabbits. Vet. Rec.,165(19):563-8.
  12. Bosch, P., Pratt, S.L. and Stice, S.L. (2006).Isolation, characterization, gene modification, and nuclear reprogramming of porcine mesenchymal stem cells. Biol.Reprod.,74:46-57.
  13. Bosnakovski, D., Mizuno, M., Kim, G., Takagi, S., Okumur, M. and Fujinag, T. (2006). Gene expression profile of bovine bone marrow mesenchymal stem cell during spontaneous chondrogenic differentiation in pellet culture system. Jpn. J. Vet. Res., 53(3-4):127-39.
  14. Brederlau, A., Correia, A.S., Anisimov, S.V., Elmi, M., Paul, G., Roybon, L., Morizane, A., Bergquist, F., Riebe, I., Nannmark, U., Carta, M., Hanse, E., Takahashi, J., Sasai, Y., Funa, K., Brundin, P., Eriksson, P.S. and Li, J.Y. (2006). Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson’s disease: effect of in vitro differentiation on graft survival and teratoma formation. Stem Cells, 24:1433-1440.
  15. Broxmeyer, H.E. (2005). Biology of cord blood cells and future prospects for enhanced clinical beneût. Cytotherapy, 7:209-218.
  16. Bruder, S.P., Kraus, K., Goldberg, V. and Kadiyala, S. (1998). The Effect of Implants Loaded with Autologous Mesenchymal Stem Cells on the Healing of Canine Segmental Bone Defects.J. Bone and Joint Surg., Incorporated 80-A:985-996.
  17. Butler, D. and Awad, H. (1999). Perspectives on cell and collagen composites for tendon repair. Clin. Orthop. Rel. Res., 367:S324–S332.
  18. Caplan, A.I. (1991). Mesenchymal stem cells. J. Orthop. Res., 9:641-650.
  19. Carvalho, A.M., Alves, A.L.G., Oliveira, P.G.G., Alvarez, L.E.C., Amorim, R.L., Hussni, C.A. and Deffune, E. (2011). Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines Journal of Equine. Vet. Sci., 31(1):26-34.
  20. Caspi, O., Huber, I., Kehat, I., Habib, M., Arbel, G., Gepstein, A., Yankelson, L., Aronson, D., Beyar, R. and Gepstein, L. (2007). Transplantation of human embryonic stem cell-derived cardiomyocytes improves myocardial performance in infarcted rat hearts. J. Am. Coll. Cardiol., 50(19):1884-93.
  21. Chen, J., Li, Y., Wang, L., Zhang, Z., Lu, D., Lu, M. and Chopp M. (2001).Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke,32: 1005-1011.
  22. Crisan,M., Yap, S., Casteilla, L., Chen, C.W., Corselli,M., Park, T.S., Andriolo, G., Sun, B., Zheng, B., and Zhang, L., et al. (2008). A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell,3:301-313.
  23. Cristofanilli, M., Harris, V.K., Zigelbaum, A., Goossens, A.M., Lu, A., Rosenthal, H. and Sadiq, S.A. (2011). Mesenchymal Stem Cells Enhance the Engraftment and Myelinating Ability of Allogeneic Oligodendrocyte Progenitors in Dysmyelinated Mice. Stem Cells Dev. (Published online).
  24. Dasari, V.R., Spomar, D.G., Gondi, C.S., Sloffer, C.A., Saving, K.L., Gujrati, M., Rao, J.S. and Dinh, D.H. (2007).Axonal remyelination by cord blood stem cells after spinal cord injury. J.Neurotrauma., 24:391–410.
  25. Chung Y, Klimanskaya I, Becker S, Marh J, Lu SJ, Johnson J, Meisner L, Lanza R. (2006). Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Nature, 439(7073):216-219.
  26. Dattena, M., Chessa, B., Lacerenza, D., Accardo, C., Pilichi, S., Mara, L., Chessa, F., Vincenti, L. and Cappai, P. (2006). Isolation, culture and characterization of embryonic cell lines from vitrified sheep blastocysts. Mol.Reprod. Dev., 73(1):31-39.
  27. Dattena, M., Pilichi, S., Rocca, S., Mara, L., Casu, S., Masala, G., Manunta, L., Manunta, A., Passino, E.S., Pool, R.R. and Cappai, P. (2009). Sheep embryonic stem-like cells transplanted in full-thickness cartilage defects. J. Tissue Eng. Regen. Med., 3(3):175-87.
  28. De Bari, C., Dell’accio, F., Tylzanowski, P., and Luyten, F.P. (2001). Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum., 44:1928-1942.
  29. DeBari, C., Dell’Accio, F., Vandenabeele, F., Vermeesch, J.R., Raymeckers, J.M. and Luten, F.P. (2003). Skeletal muscle repair by adult human mesenchymal stem cells from synovial membranes. J. Cell Biol., 160(6):807-809.
  30. Deng, Y.B., Liu, X.G., Liu, Z.G., Liu, X.L., Liu, Y. and Zhou, G.Q. (2006). Implantation of BM mesenchymal stem cells into injured spinal cord elicits de novo neurogenesis and functional recovery: evidence from a study in rhesus monkeys. Cytotherapy, 8:210–214.
  31. Dennis, J.E., Carbillet, J.P., Caplan, A.I. and Charbord, P. (2002). The STRO-1+ marrow cell population is multipotential. Cells Tiss. Org., 170:73-82.
  32. Dounchis, J., Bae, W., Chen, A., Sah, R., Coutts, R., and Amiel, D. (2000). Cartilage repair with autogenic perichondrium cell and polylactic acid grafts. Clin. Orthop. Rel. Res., 337:248-264.
  33. Eslaminejad, M.B., Taghiyar, L., Dehghan, M.M., Falahi, F. and Kazemi, H. (2009). Equine marrow-derived mesenchymal stem cells: Isolation, differentiation and culture optimization. Iranian J. Vet. Res., 26:1-11.
  34. Evans, M.J. and Kaufman, M.H. (1981). Establishment in culture of pluripotential cells from mouse embryos. Nature, 292(5819):154-156.
  35. Fortier, L.A., Potter, H.G., Rickey, E.J., Schnabel, L.V., Foo, L.F., Chong, L.R., Stokol, T., Cheetham, J. and Nixon, A.J. (2010). Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model. J. Bone Joint Surg. Am., 92(10):1927-37.
  36. Friedenstein, A.J., Chailakhjan, R.K., and Lalykina, K.S. (1970). The development of ûbroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet., 3:393-403.
  37. Frisbie, D.D. and Smith, R.K. (2010).Clinical update on the use of mesenchymal stem cells in equine orthopaedics. Equine Vet. J.,42(1):86-9.
  38. Gatti, R., Hilaire, A., Meuwissen, J., Allen, H. D., Hong, R. and Good R. A. (1968).Immunological reconstitution of sex-linked lymphopenic immunological deûciency Lancet, 2:1366-1369.
  39. Fukuda, H. and Takahashi, J. (2005). Embryonic stem cells as a cell source for treating Parkinson’s disease. Expert. Opin. Biol. Ther.,5:1273-1280.
  40. Herthel, D.J. (2001). Enhanced suspensory ligament healing in 100 horses by stem cell and other bone marrow components. A.A.E.P. Pro., 47:319-21.
  41. Huang, B., Li, T., Wang, X.L., Xie, T.S., Lu, Y.Q., Silva, F.M. and Shi, D.S. (2010). Generation and characterization of embryonic stem-like cell lines derived from in vitro fertilization Buffalo (Bubalusbubalis) embryos. Reprod. Domes.Ani., 45(1):122-128.
  42. Iannaccone, P.M., Taborn, G.U., Garton, R.L., Caplice, M.D. and Brenin, D.R. (1994). Pluripotent embryonic stem cells from the rat are capable of producing chimeras. Dev. Biol., 163:288-292.
  43. Igura, K., Zhang, X., Takahashi, K., Mitsuru, A., Yamaguchi, S., and Takashi, T.A. (2004).Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta. Cytotherapy, 6:543-553.
  44. Jakobs, P.M., Smith, L., Thayer, M. and Grompe, M. (1999). Embryonic stem cell can be used to conduct hybrid cell lines containing a single, selectable murine chromosome. Mamm. Genome, 10: 381-384.
  45. Jeong, J.H., Ki, Y.W., Kim, J.Y., Jan, S.H., Kim, S.H. and Chang, Y. (2005). Adipose Tissue Derived MSC Enhances Motor Function in Rats with Cerebral Infarction. IFATS. Oral Presentation.
  46. Kadiyala, S., Jaiswal, N., and Bruder, S. P. (1997). Culture-expanded, bone marrow-derived mesenchymal stem cells can regenerate a critical- sized segmental bone defect. Tissue Eng., 3:173-185.
  47. Koch, T.G., Berg, L.C. and Betts, D.H. (2009). Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Can. Vet. J., 50(2):155-65.
  48. Kon, E., Muraglia, A., Corsi, A., Bianco, P., Marcacci, M., Martin, I., Boyde, A., Ruspantini, I., Chistolini, P., Rocca, M., Giardino, R., Cancedda, R. and Quarto, R. (2000). Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones. J. Biomed. Mater. Res., 49(3):328–337.
  49. Kraus, K.H. and Kirker, C. (2006). Mesenchymal stem cell and bone regeneration. Vet. surg., 35:232-242.
  50. Krause, D. S., Theise, N. D., Collector, M. I., Hengariu, O., Hwang, S., Gardner, R., Neutzel, S., and Sharkis, S. J. (2001).Multi-organ, multi-lineage engraftment by a single bone marrow–derived stem cell. Cell, 105:369-377.
  51. Laflamme, M.A., Gold, J., Xu, C., Hassanipour, M., Rosler, E., Police, S., Muskheli, V. and Murry, C.E. (2005). Formation of human myocardium in the rat heart from human embryonic stem cells. Am. J. Pathol.,167:663-671.
  52. Laura, C., Groza, I., Oana, L., Pall, E., Tean, C. P., Catana, P. and Cenariu, M. (2008). Canine mesenchymal stem cells isolation from bone marrow aspirates. Bulletin UASVM, Vet. Med.,65(2):96-101.
  53. Lechner, A. (2004). Stem cells and regenerative medicine for the treatment of type 1 diabetes: the challenges lying ahead. Pediatr. Diabetes, 5:88-93.
  54. Lee, K.B., Choi, J., Cho, S.B., Chung, J.Y., Moon, E.S., Kim, N.S. and Han, H.J. (2011). Topical embryonic stem cells enhance wound healing in diabetic rats. J. Orthop. Res.,29: N/A. doi: 10.1002/jor.21385.
  55. Lim, J.H., Byeon, Y.E., Ryu, H.H., Jeong, Y.H., Lee, Y.W., Kim, W.H., Kang, K.S. and Kweon, O.K. (2007). Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs. J. Vet. Sci.8:275–282.
  56. Liu X, Li X, Fan Y, Zhang G, Li D, Dong W, Sha Z, Yu X, Feng Q, Cui F, Watari F. (2010). Repairing goat tibia segmental bone defect using scaffold cultured with mesenchymal stem cells. J. Biomed.Mater. Res. B. Appl. Biomater. 94(1):44-52.
  57. Lohnes, D. (1999). Gene targeting of retinoid receptors. Mol. Biotechnol., 11: 67-84.
  58. Mackenzie, T.C. and Flake, A.W. (2001). Human mesenchymal stem cells persist, demonstrate site-specific multipotential differentiation, and are present in sites of wound healing and tissue regeneration after transplantation into fetal sheep. Blood Cells, Mol. &Dis., 27(3):601-604.
  59. Martin, D.R., Cox, N.R., Hathcock, T.L., Niemeyer, G.P., and Baker, H.J. (2002).Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow. Exptl. Hematol.,30:879-886.
  60. Menard, C., Hagege, A.A. and Agbulut, O. (2005). Transplantation of cardiac-committed mouse embryonic stem cells to infarcted sheep myocardium: a preclinical study. Lancet, 366:1005-12.
  61. Min, J., Yang, Y., Sullivan, M.F., Ke, Q., Converso, K.L., Chen, Y., Morgan, J.P. and Xiao, Y. (2003). Long-term improvement of cardiac function in rats after infarction by transplantation of embryonic stem cells. J. Thorac. Cardiovasc. Surg., 125:361-369.
  62. Mitalipova, M., Beyhan, Z. and First, N.L. (2001). Pluripotency of bovine embryonic cell line derived from precompacting embryos. Cloning, 3(2):59-67.
  63. Murphy, J. M., Fink, D. J., Hunziker, E. B. and Barry, F. P. (2003). Stem cell therapy in a caprine model of osteoarthritis. Arthritis & Rheumatism, 48:3464-3474.
  64. Modlinski, J.A., Michael, A.R., Thomas, E.W. and Jolanta, K. (1996). Embryonic stem cell: Developmental capabilities and their possible use in mammalian embryo cloning. Anim. Reprod. Sci. 42: 437-446.
  65. Nair, M.B., Varma, H.K., Menon, K.V., Shenoy, S.J. and John, A. (2009).Reconstruction of goat femur segmental defects using triphasic ceramic-coated hydroxyapatite in combination with autologous cells and platelet-rich plasma. Acta Biomater., 5(5):1742-55.
  66. Nixon, A.J., Dahlgren, L.A., Haupt, J.L., Yeager, A.E., Ward, D.L. (2008). Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis. Am. J. Vet. Res.,69(7):928-37.
  67. Notarianni, E., Galli, C., Moor, R.M. and Evans, M.J. (1991). Derivation of pluripotent, embryonic cell lines from the pig and sheep blastocysts. J. Reprod. Fertil. Suppl., 43:255-260.
  68. Oloumi, M.M., Derakhshanfar, A., Shemali, H. and Shavalian, M. (2008). The Role of Autogenous Bone Marrow in the Healing of Experimental Burn Wound Healing in Rabbits. Iranian J. Vet. Surg., 3(2): 47-55.
  69. Orlic, D., Kajstura, J., Chimenti, S.,Jakoniuk, I., Anderson, S.M., Li, B., Pickel, J., McKay, R., Nadal-Ginard, B., Bodine, D.M., Leri, A. and Anversa, P. (2001). Bone marrow cells regenerate infarcted myocardium. Nature, 410:701-5.
  70. Passier, R. and Mummery, C. (2003). Origin and use of embryonic stem cells in differentiation and tissue repair. Cardiovasc. Res., 58(2): 324-325.
  71. Piedrahita, J.A., Anderson, G.B. and BonDurant, R.H. (1990). On the isolation of embryonic stem cells: Comparative behavior of murine, porcine and ovine embryos. Theriogenology, 34: 879-901.
  72. Rambhatle, L., Chiu, C.P., Kundu, P., Peng, V. and Carpenter, M.K. (2003). Generation of hepatocyte like cells from human embryonic stem cells. Cell Transplant., 12(1): 1-11.
  73. Ribitsch, I., Burk, J., Delling, U., Geibler, C., Gittel, C., Julke, H. and Brehm, W. (2010). Basic Science and Clinical Application of Stem Cells in Veterinary Medicine.Adv. Biochem. Eng. Biotechnol., 123:219-263.
  74. Rogers, I., and Casper, R.F. (2004).Umbilical cord blood stem cells. Best Pract. Res. Clin. Obstet. Gynaecol., 18:893-908.

Editorial Board

View all (0)