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Current IssueEditorial BoardOnline First ArticlesArchive

volume 23 issue 1 (march 2002) : 53 - 58

THE PHYSIOLOGICAL STATUS OF MICROPROPAGATED PLANTS - A REVIEWQ

B
B.N. Hazarikat
V
V.A. Parthasarathy2
G
G. Bhowmik3
1Biotechnology Laboratory, Division of Horticulture ICAR Research Complex for NEH Region, Umiam - 793103, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Hazarikat B.N., Parthasarathy2 V.A., Bhowmik3 G. (2025). THE PHYSIOLOGICAL STATUS OF MICROPROPAGATED PLANTS - A REVIEWQ. Agricultural Reviews. 23(1): 53 - 58. doi: .

ABSTRACT

Tissue cultured shoots or plantlets share certain characteristic features that are inconsistant with the development under greenhouse or field conditions. The greenhouse and field have substantially lower relative humidities, higher light levels and septic environments that are stressfull to micropropagated plants compared to in vitro conditions. The benefits of any micropropagation system can, however, only be fully realised by the successful transfer of plantlets from tissue culture vessels to the ambient conditions found ex vitro. The tissue culture plants are often characterised by abnormal leaf morphology and anatomy, poor photosynthetic efficiency, malfunctioning of stomata and a marked decrease in epicuticular waxes. This review will examine the anatomical, morphological and physiological factors that account for the fragility of cultured plants when removed from culture.

KEYWORDS

    REFERENCES

    1. Blanke, M.B. and Belcher, AR. (1989). PI. Cell Tiss. arg. Cult. 19: 85-89.
    2. Brainerd, KE. and Fuchigami, L.H. (1981). JAm. Soc. Hort. Sci. 106 : 515-518.
    3. Brainerd, KE. etal. (1981). Hort. Sci. 16: 173-175.
    4. Brainerd, KE. and Fuchigami, L.H. (1982). J.Exp. Bot. 33 : 388-392.
    5. Cappellades, M. etal. (1990). Photosynthetica. 24 : 190-193.
    6. Capellades, M. etal. (1991). PI. cell. nss. Org. Cult. 25: 21-26.
    7. Conner, AJ. and Thomas, M.P. (1982). Proc./nter. Plant. Prop.Soc. 31 : 342-357.
    8. Conner, AJ. and Conner, A.J. (1984). PI. Sci. Lett. 36 : 241-246.
    9. Desjardins, Y. etal. (1987). J.Am. Soc. Hort. Sci. 112: 846-851.
    10. Desjardins, Y. et al. (1988). Acta Hort. 230 : 45-53.
    11. Dhawan, V. and Bhojwani, G. (1988). J. Hort. Sci. 63 : 393 -405.
    12. Donnelly, D.J. et al. (1984). PI. ceO Tiss. Org. Cult. 3 : 313-317.
    13. Donnelly, D.J. and Vidaver, W.E. (1984a). J. Am.Soc. Hort. Sci. 109: 177-181.
    14. Donnelly, D.J. et al. (1985). PI. CeU Tiss. Org. Cult. 4 : 43-50.
    15. Fabbri, A. et aI. (1986). Scientia Hort. 28 : 331-337.
    16. Fuchigami, L.H. etal. (1981). J. Am. Soc. Hort. Sci. 106 : 519-522.
    17. Fujiwara, Ketal (1987). J. Agric. Met. 43: 21-30.
    18. Fujiwara. K et al (1988). Acta. Hort. 230 : 153-158.
    19. Grout, B. W.w. (1975) PI. Sci. Lett. 5: 401-405.
    20. Grout, B. W.w. and Crisp. P. (1977). Acta. Hort. 79 : 289 -296.
    21. Grout, B. W. W. and Aston, M. J. (1977a). Hort. Res. 17 : 1-7.
    22. Grout, B. W. W. and Aston, M.J. (1977b). Hort. Res. 17: 65-71.
    23. Grout, B. W. W. and Aston, M.J. (1978) Ann. Bot. 42: 993 -995.
    24. Grout, B. W. W. and MilIam, S. (1985). Ann. Bot. 55 : 129 -131.
    25. Grout, B. W. W. and Donkin, M.E. (1987). ActaHort. 212: 323 -323.
    26. Grout, B. W. W. (1988). Acta. Hort. 230: 129 -135.
    27. Hazarika, B.N. (1999). Ph. D. Thesis, Gauhati University, India.
    28. Koch, KE. (1996). Annu. Rev. PI. Physiol. PI. Mol. BioI. 47 : 509 -540. :
    29. Kovtun, Y. and Daie, J. (1995) PI. Physiol. 108: 1647 -1657.
    30. Kozai, T et al. (1987a). J. Agric. Met. Jap. 42 : 349 -358.
    31. Kozai, T. et al (1987b). PI. Tissue Culture lett. 4 : 22 -26.
    32. Kozai, T. etal (1988). Acta. Hort. 230: 121-127.
    33. Kozai, T. (1990). Proc. Int. Symp. on Application of Biotechnology for SlTillllndustries, Bangkok. PP. 39-51.
    34. Kozai, T. etal (1991). PI. ceO Tissue. Org. Cult. 24 : 187 -191.
    35. 58 AGRICULTURAL REVIEWS
    36. Martin, J.T. and Juniper, B.E. (1970). The Cutides ofPlants. St. Martin 50 Press, New York.
    37. Martin, J.A. et al (1988). Ann. Bot. 62 : 663 -670.
    38. Miguens, F.e. etal (1993). Pl Cell Tiss. Org. Cult. 32: 109 -113.
    39. Pamplin, E. J. and Chapman, J.M. (1975). J. Exp. Bot. 26 ': 212 - 220.
    40. Saroj. P.L. et al (1977). Indian J. Hort. 54 : 198-204.
    41. ShaekeI, K A. et al (1990). J. Am. Soc. Hort. Sci. 115: 468 - 472.
    42. Sims, DA and Pearcy. RW. (19'91). Oecologia. 85 : 447 -453.
    43. Smith, M. A.L. et al (1986). JAm. Soc. Hort. Sci. 111 : 437 - 442.
    44. Storey, R and Treeby, M.T. (1994). J. Hort. Sci. 69 : 329 -338.
    45. Sutter, E. and Langhans. R W. (1979). J. Am. Soc. Hort. Sci. 104: 493-496.
    46. Sutter, E. and Langhans, RW. (1982). Can. J. Bot. 62: 74-77.
    47. Sutter, E. (1984). Can. J. Bot. 62 : 74-77.
    48. Sutter, E. (1988). J. Am. Soc. Hort. Sci. 113 : 234 -238.
    49. Ticha, I. et al (1998). Physiol Plant. 102: 155 - 162.
    50. Wardle, K. (1983). J. Am. Soc. Hort. Sci. 108: 386 -389.
    51. Wetzstein, H.Y. and Sommer, H.E. (1982). Am. J. Bot. 69 : 1579 -1586.
    52. Wetzstein, HY and Sommer, H.E. (1983). J. Am. Soc, Hart. Sci. 108: 475 -480
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      volume 23 issue 1 (march 2002) : 53 - 58

      THE PHYSIOLOGICAL STATUS OF MICROPROPAGATED PLANTS - A REVIEWQ

      B
      B.N. Hazarikat
      V
      V.A. Parthasarathy2
      G
      G. Bhowmik3
      1Biotechnology Laboratory, Division of Horticulture ICAR Research Complex for NEH Region, Umiam - 793103, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Hazarikat B.N., Parthasarathy2 V.A., Bhowmik3 G. (2025). THE PHYSIOLOGICAL STATUS OF MICROPROPAGATED PLANTS - A REVIEWQ. Agricultural Reviews. 23(1): 53 - 58. doi: .

      ABSTRACT

      Tissue cultured shoots or plantlets share certain characteristic features that are inconsistant with the development under greenhouse or field conditions. The greenhouse and field have substantially lower relative humidities, higher light levels and septic environments that are stressfull to micropropagated plants compared to in vitro conditions. The benefits of any micropropagation system can, however, only be fully realised by the successful transfer of plantlets from tissue culture vessels to the ambient conditions found ex vitro. The tissue culture plants are often characterised by abnormal leaf morphology and anatomy, poor photosynthetic efficiency, malfunctioning of stomata and a marked decrease in epicuticular waxes. This review will examine the anatomical, morphological and physiological factors that account for the fragility of cultured plants when removed from culture.

      KEYWORDS

        REFERENCES

        1. Blanke, M.B. and Belcher, AR. (1989). PI. Cell Tiss. arg. Cult. 19: 85-89.
        2. Brainerd, KE. and Fuchigami, L.H. (1981). JAm. Soc. Hort. Sci. 106 : 515-518.
        3. Brainerd, KE. etal. (1981). Hort. Sci. 16: 173-175.
        4. Brainerd, KE. and Fuchigami, L.H. (1982). J.Exp. Bot. 33 : 388-392.
        5. Cappellades, M. etal. (1990). Photosynthetica. 24 : 190-193.
        6. Capellades, M. etal. (1991). PI. cell. nss. Org. Cult. 25: 21-26.
        7. Conner, AJ. and Thomas, M.P. (1982). Proc./nter. Plant. Prop.Soc. 31 : 342-357.
        8. Conner, AJ. and Conner, A.J. (1984). PI. Sci. Lett. 36 : 241-246.
        9. Desjardins, Y. etal. (1987). J.Am. Soc. Hort. Sci. 112: 846-851.
        10. Desjardins, Y. et al. (1988). Acta Hort. 230 : 45-53.
        11. Dhawan, V. and Bhojwani, G. (1988). J. Hort. Sci. 63 : 393 -405.
        12. Donnelly, D.J. et al. (1984). PI. ceO Tiss. Org. Cult. 3 : 313-317.
        13. Donnelly, D.J. and Vidaver, W.E. (1984a). J. Am.Soc. Hort. Sci. 109: 177-181.
        14. Donnelly, D.J. et al. (1985). PI. CeU Tiss. Org. Cult. 4 : 43-50.
        15. Fabbri, A. et aI. (1986). Scientia Hort. 28 : 331-337.
        16. Fuchigami, L.H. etal. (1981). J. Am. Soc. Hort. Sci. 106 : 519-522.
        17. Fujiwara, Ketal (1987). J. Agric. Met. 43: 21-30.
        18. Fujiwara. K et al (1988). Acta. Hort. 230 : 153-158.
        19. Grout, B. W.w. (1975) PI. Sci. Lett. 5: 401-405.
        20. Grout, B. W.w. and Crisp. P. (1977). Acta. Hort. 79 : 289 -296.
        21. Grout, B. W. W. and Aston, M. J. (1977a). Hort. Res. 17 : 1-7.
        22. Grout, B. W. W. and Aston, M.J. (1977b). Hort. Res. 17: 65-71.
        23. Grout, B. W. W. and Aston, M.J. (1978) Ann. Bot. 42: 993 -995.
        24. Grout, B. W. W. and MilIam, S. (1985). Ann. Bot. 55 : 129 -131.
        25. Grout, B. W. W. and Donkin, M.E. (1987). ActaHort. 212: 323 -323.
        26. Grout, B. W. W. (1988). Acta. Hort. 230: 129 -135.
        27. Hazarika, B.N. (1999). Ph. D. Thesis, Gauhati University, India.
        28. Koch, KE. (1996). Annu. Rev. PI. Physiol. PI. Mol. BioI. 47 : 509 -540. :
        29. Kovtun, Y. and Daie, J. (1995) PI. Physiol. 108: 1647 -1657.
        30. Kozai, T et al. (1987a). J. Agric. Met. Jap. 42 : 349 -358.
        31. Kozai, T. et al (1987b). PI. Tissue Culture lett. 4 : 22 -26.
        32. Kozai, T. etal (1988). Acta. Hort. 230: 121-127.
        33. Kozai, T. (1990). Proc. Int. Symp. on Application of Biotechnology for SlTillllndustries, Bangkok. PP. 39-51.
        34. Kozai, T. etal (1991). PI. ceO Tissue. Org. Cult. 24 : 187 -191.
        35. 58 AGRICULTURAL REVIEWS
        36. Martin, J.T. and Juniper, B.E. (1970). The Cutides ofPlants. St. Martin 50 Press, New York.
        37. Martin, J.A. et al (1988). Ann. Bot. 62 : 663 -670.
        38. Miguens, F.e. etal (1993). Pl Cell Tiss. Org. Cult. 32: 109 -113.
        39. Pamplin, E. J. and Chapman, J.M. (1975). J. Exp. Bot. 26 ': 212 - 220.
        40. Saroj. P.L. et al (1977). Indian J. Hort. 54 : 198-204.
        41. ShaekeI, K A. et al (1990). J. Am. Soc. Hort. Sci. 115: 468 - 472.
        42. Sims, DA and Pearcy. RW. (19'91). Oecologia. 85 : 447 -453.
        43. Smith, M. A.L. et al (1986). JAm. Soc. Hort. Sci. 111 : 437 - 442.
        44. Storey, R and Treeby, M.T. (1994). J. Hort. Sci. 69 : 329 -338.
        45. Sutter, E. and Langhans. R W. (1979). J. Am. Soc. Hort. Sci. 104: 493-496.
        46. Sutter, E. and Langhans, RW. (1982). Can. J. Bot. 62: 74-77.
        47. Sutter, E. (1984). Can. J. Bot. 62 : 74-77.
        48. Sutter, E. (1988). J. Am. Soc. Hort. Sci. 113 : 234 -238.
        49. Ticha, I. et al (1998). Physiol Plant. 102: 155 - 162.
        50. Wardle, K. (1983). J. Am. Soc. Hort. Sci. 108: 386 -389.
        51. Wetzstein, H.Y. and Sommer, H.E. (1982). Am. J. Bot. 69 : 1579 -1586.
        52. Wetzstein, HY and Sommer, H.E. (1983). J. Am. Soc, Hart. Sci. 108: 475 -480
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