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Current IssueEditorial BoardOnline First ArticlesArchive

volume 28 issue 2 (june 2007) : 79-92

ROLE OF GREEN FLORESCENT PROTEIN (GFP) GENE IN SOMATIC CELL CULTURES - A REVIEW

A
Ashwani Sharma
A
Ashish Chopra
1Dairy Cattle Breeding Division National Dairy Research Institute- Karnal-132001, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Sharma Ashwani, Chopra Ashish (2025). ROLE OF GREEN FLORESCENT PROTEIN (GFP) GENE IN SOMATIC CELL CULTURES - A REVIEW. Agricultural Reviews. 28(2): 79-92. doi: .

ABSTRACT

Green fluorescent protein (GFP) is a single peptide of 238 amino acids, extracted from Aequorea jelly fish absorbs blue light and emits green light. Cloning of GFP came with breakthrough that expression of the gene in other organisms aeates fluorescence and there is no jellyfish specific enzymes needed for the post-translational synthesis of the chromofore. The GFP is a noninvasive, effective marker for screening and allows the direct visualization of gene expression in living cells without the need for destructive techniques or the addition of cofactors. Prior to done any livestock species for the purpose to fulfill the requirement of human need, the incorporation of transgene through transfection in the somatic cells is essential. Desired gene can be inserted in the somatic cells and actual transfected cells can be identified with the help of any reporter gene among them GFP is most popular due to its independence from any substrate or cofactor, and these cells can be separated through any suitable technique such as Auorescent Activated Cell Sorting (FACS). Even very few no. of transfected somatic cells can lead to successful generation of Transgenic animal. 

KEYWORDS

    REFERENCES

    1. Almofti M.R., et al. (2003). Mol Membrane Biof, 20: 35-43.
    2. Andreason, GLand Evans, G.A. (1988). BioTechniques 6: 650-660.
    3. Andreason, G.L.. and Evans, G.A. (1989). Analytical Biochem. 180:269-275.
    4. Arat, S. et al.(2oo2). BioI. Reproduction 66: 1768-1774.
    5. Atkins, R.L.et al. (2000). Biotechniques 28(1): 1-5.
    6. Ayoob, J.C et al.(2ooo). MolBiotech. 17: 167-184.
    7. Baguisi, A et al. (1999). Nature Biotech.17: 456-461.
    8. Bhuiyan, M. M. U.,et al. (2004). J. Vet. Med. Sci. 66(3): 257-261.
    9. Breje K., et al. (1997). Proc. Nail. Acad. Sci. USA 94:2306-2311.
    10. Bondioli K, etal.(2oo1). Mol. Reprod. Dev. 60:189-195.
    11. Capechhi, MR, (1980). Cell 22: 479-488.
    12. Catalado, L. M., et al. (1998). In: Cell Culturing. (Katya Ravid and R. Ian Freshney ed). Wiley-Liss Inc. 241-54.
    13. Chalfie, M.,etal. (1994). Science. 263:802-805
    14. Chen, SH., etdl.(2oo2). BioI. Reprod. 67,1488--1492.
    15. Chopra,A and Sharma,A(2005) NDRI News L 10:2-3
    16. Chu, G., et al.(1987). Nucleic Acids Res. 15:1311-1326.
    17. Cibelli, J.8., et al.(1998) Science, 280 : 1256-58.
    18. Cubitt, A B., et al.(1995). Science. 20 (11):448-455.
    19. Diaz, M.A.M., et al. (2003). J. Vet. M'Jd. Sci. 65: 989-994.
    20. Eibs, H.G., and Speilman, H. (1977). Radiat. Res. 71(2):367-376.
    21. Elnitski, L. and Hardison, R. (1999) Blood Celis, Molecules, and Diseases 25(19): 299-304.
    22. Feigner P., et al.(1987) Proc Nail Acad Sci USA; 84: 7413-7417.
    23. Gerdes, H., and Kaether, C. (1996). FEBS Letters. 389: 44-47.
    24. Graessmann, M., Graessmann, A (1976). Proc. Nail. Acad. Sci. USA 79: 6777-6781.
    25. Hadjantonakis, A K.,et al.(1998). MechanismsDevelop. 76:79-90.
    26. Hill, J.R., et al. (2000). Biol.Reprod. 62: 1135-1140.
    27. Hyun, S., et al. (2003). BioI. Reprod. 69: 1360-1368.
    28. lkawa, M.,et al.(1995) FEBS Letters. 375: 125-128.
    29. Kalina, J.,et al. (2003). Czech. J. Anim. Sci., 48: 279-284.
    30. Kato, M., et al.(1999) Mol. Reprod. Dev. 54: 43-48.
    31. Keefer, C.L., et al. (2001). BioIReprod., 64, 849-856.
    32. Koo, 0.8., et al.(2001).Mol. Rerpod. Devp. 58: 15-21.
    33. Lanza, R.P.,et al.(2000) Cloning 2: 79-90.
    34. Lascombe, I.,et al. (1996) BioTechniques 20: 88-91.
    35. Li, L.H., et al.(2002). Tech. in Cancer Res. and Treat. 1: 341-349.
    36. Li, S.,et aJ..(2002). J. Nail. Cancer Inst. 94: 762-768.
    37. Li, x., etal.(1997). J. BioI. Chern. 272:2855-2859.
    38. Lin, C.R., et al.(2oo2). Neuroscience Letters 317: 1-4.
    39. Mohr, L.R., and Trounson, A 0. (1980) J. Reprod. Fertil. 58(1):189-96.
    40. Naito, M.,. (2000) Anirn. Sci. J. 71(4): 377-385.
    41. Onishi, A, (2000) Science 289: 1188-1190. 92 AGRICULTURAL REVIEWS
    42. Paulssen, R.I.,et al.(1990). Biochem. Biophy. Res. Comm. 171(3): 1029-1036.
    43. Polejaeva,l.A.,et a/. (2000) Nature 407: 86-90.
    44. Potter, H. (1996)., Current Protocol on Molecular Biology. John Wiley &Sons, Inc., New York, 931-936.
    45. Schnieke AE., et a/. (1997). Science 278:2130-2133.
    46. Shimomura et al (1962) J. cell. Compo Physiol. 59: 223-39.
    47. Thoma>, J.L., (2003). Insect Sci, 3:17.
    48. Waki'lyama, T., etaJ. (1998). Nature 394: 369-74.
    49. Waki'lyama, T.,etal.(1999). Proc. Nat. A. Soc. 96: 14984-89.
    50. Wells, D.N., etaJ.( 1997). Bio/. Reprod. 57: 385-93.
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      volume 28 issue 2 (june 2007) : 79-92

      ROLE OF GREEN FLORESCENT PROTEIN (GFP) GENE IN SOMATIC CELL CULTURES - A REVIEW

      A
      Ashwani Sharma
      A
      Ashish Chopra
      1Dairy Cattle Breeding Division National Dairy Research Institute- Karnal-132001, India.

      • Submitted|

      • First Online |

      • doi

      Cite article:- Sharma Ashwani, Chopra Ashish (2025). ROLE OF GREEN FLORESCENT PROTEIN (GFP) GENE IN SOMATIC CELL CULTURES - A REVIEW. Agricultural Reviews. 28(2): 79-92. doi: .

      ABSTRACT

      Green fluorescent protein (GFP) is a single peptide of 238 amino acids, extracted from Aequorea jelly fish absorbs blue light and emits green light. Cloning of GFP came with breakthrough that expression of the gene in other organisms aeates fluorescence and there is no jellyfish specific enzymes needed for the post-translational synthesis of the chromofore. The GFP is a noninvasive, effective marker for screening and allows the direct visualization of gene expression in living cells without the need for destructive techniques or the addition of cofactors. Prior to done any livestock species for the purpose to fulfill the requirement of human need, the incorporation of transgene through transfection in the somatic cells is essential. Desired gene can be inserted in the somatic cells and actual transfected cells can be identified with the help of any reporter gene among them GFP is most popular due to its independence from any substrate or cofactor, and these cells can be separated through any suitable technique such as Auorescent Activated Cell Sorting (FACS). Even very few no. of transfected somatic cells can lead to successful generation of Transgenic animal. 

      KEYWORDS

        REFERENCES

        1. Almofti M.R., et al. (2003). Mol Membrane Biof, 20: 35-43.
        2. Andreason, GLand Evans, G.A. (1988). BioTechniques 6: 650-660.
        3. Andreason, G.L.. and Evans, G.A. (1989). Analytical Biochem. 180:269-275.
        4. Arat, S. et al.(2oo2). BioI. Reproduction 66: 1768-1774.
        5. Atkins, R.L.et al. (2000). Biotechniques 28(1): 1-5.
        6. Ayoob, J.C et al.(2ooo). MolBiotech. 17: 167-184.
        7. Baguisi, A et al. (1999). Nature Biotech.17: 456-461.
        8. Bhuiyan, M. M. U.,et al. (2004). J. Vet. Med. Sci. 66(3): 257-261.
        9. Breje K., et al. (1997). Proc. Nail. Acad. Sci. USA 94:2306-2311.
        10. Bondioli K, etal.(2oo1). Mol. Reprod. Dev. 60:189-195.
        11. Capechhi, MR, (1980). Cell 22: 479-488.
        12. Catalado, L. M., et al. (1998). In: Cell Culturing. (Katya Ravid and R. Ian Freshney ed). Wiley-Liss Inc. 241-54.
        13. Chalfie, M.,etal. (1994). Science. 263:802-805
        14. Chen, SH., etdl.(2oo2). BioI. Reprod. 67,1488--1492.
        15. Chopra,A and Sharma,A(2005) NDRI News L 10:2-3
        16. Chu, G., et al.(1987). Nucleic Acids Res. 15:1311-1326.
        17. Cibelli, J.8., et al.(1998) Science, 280 : 1256-58.
        18. Cubitt, A B., et al.(1995). Science. 20 (11):448-455.
        19. Diaz, M.A.M., et al. (2003). J. Vet. M'Jd. Sci. 65: 989-994.
        20. Eibs, H.G., and Speilman, H. (1977). Radiat. Res. 71(2):367-376.
        21. Elnitski, L. and Hardison, R. (1999) Blood Celis, Molecules, and Diseases 25(19): 299-304.
        22. Feigner P., et al.(1987) Proc Nail Acad Sci USA; 84: 7413-7417.
        23. Gerdes, H., and Kaether, C. (1996). FEBS Letters. 389: 44-47.
        24. Graessmann, M., Graessmann, A (1976). Proc. Nail. Acad. Sci. USA 79: 6777-6781.
        25. Hadjantonakis, A K.,et al.(1998). MechanismsDevelop. 76:79-90.
        26. Hill, J.R., et al. (2000). Biol.Reprod. 62: 1135-1140.
        27. Hyun, S., et al. (2003). BioI. Reprod. 69: 1360-1368.
        28. lkawa, M.,et al.(1995) FEBS Letters. 375: 125-128.
        29. Kalina, J.,et al. (2003). Czech. J. Anim. Sci., 48: 279-284.
        30. Kato, M., et al.(1999) Mol. Reprod. Dev. 54: 43-48.
        31. Keefer, C.L., et al. (2001). BioIReprod., 64, 849-856.
        32. Koo, 0.8., et al.(2001).Mol. Rerpod. Devp. 58: 15-21.
        33. Lanza, R.P.,et al.(2000) Cloning 2: 79-90.
        34. Lascombe, I.,et al. (1996) BioTechniques 20: 88-91.
        35. Li, L.H., et al.(2002). Tech. in Cancer Res. and Treat. 1: 341-349.
        36. Li, S.,et aJ..(2002). J. Nail. Cancer Inst. 94: 762-768.
        37. Li, x., etal.(1997). J. BioI. Chern. 272:2855-2859.
        38. Lin, C.R., et al.(2oo2). Neuroscience Letters 317: 1-4.
        39. Mohr, L.R., and Trounson, A 0. (1980) J. Reprod. Fertil. 58(1):189-96.
        40. Naito, M.,. (2000) Anirn. Sci. J. 71(4): 377-385.
        41. Onishi, A, (2000) Science 289: 1188-1190. 92 AGRICULTURAL REVIEWS
        42. Paulssen, R.I.,et al.(1990). Biochem. Biophy. Res. Comm. 171(3): 1029-1036.
        43. Polejaeva,l.A.,et a/. (2000) Nature 407: 86-90.
        44. Potter, H. (1996)., Current Protocol on Molecular Biology. John Wiley &Sons, Inc., New York, 931-936.
        45. Schnieke AE., et a/. (1997). Science 278:2130-2133.
        46. Shimomura et al (1962) J. cell. Compo Physiol. 59: 223-39.
        47. Thoma>, J.L., (2003). Insect Sci, 3:17.
        48. Waki'lyama, T., etaJ. (1998). Nature 394: 369-74.
        49. Waki'lyama, T.,etal.(1999). Proc. Nat. A. Soc. 96: 14984-89.
        50. Wells, D.N., etaJ.( 1997). Bio/. Reprod. 57: 385-93.
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