DOI: 10.5958/j.0976-0741.34.3.001    | Article Id: ARCC258 | Page : 163-175
Citation :- ENTOMOPATHOGENIC NEMATODES - A REVIEW.Agricultural Reviews.2013.(34):163-175
Sumit Vashisth*, Y.S. Chandel and P. K. Sharma sumitvashisth_hpau@yahoo.co.in
Address : Department of Entomology, Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 176 062, India


The entomopathogenic nematodes possessing balanced biological control attributes belong to genera Steinernema and Heterorhabditis and are having mutualistic association with bacteria of the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. Entomopathogenic nematodes being highly lethal to many important insect-pests, are safe to non-target organisms and working with their symbiotic bacteria kill the insects within 24-28 hours as compared to days and weeks required for insect killing in other biological control agents. Their infective juveniles (IJs) have been reported to tolerate short-term exposure to many chemical and biological insecticides, fungicides, herbicides, fertilizers and growth regulators, hence providing an opportunity of tank-mixing and application together. Entomopathogenic nematodes are also reported to be compatible with a number of agrochemicals and their use can offer a cost-effective alternative to pest control. Only twelve species out of nearly eighty identified Steinernematids and Heterorhabditis nematode species have been commercialized. The EPNs have the great potential to be used in  integrated pest management systems and work  done have been reviewed in this article to facilitate the students and researchers to have an overview of the work done and proceed further to undertake the advanced research in different aspects related to entomopathogenic nematodes.


Entomopathogenic nematodes (EPN) Heterorhabditis Photorhabdus Steinernema Xenorhabdus.


  1. Akhurst, R. and K. Smith. (2002). Regulation and safety. In: Gaugler, R. (Ed.), Entomopathogenic Nematology. CABI, New York, NY, pp-311-332.
  2. Akhurst, R.J., (1990). Safety to nontarget invertebrates of nematodes of economically important pests. In Safety of Microbial pp. 234-238. Boca Raton, FL: CRC 259 pp.
  3. Akhurst, R.J., Bedding, R.A., Bull, R.M. and Smith, D.R.J. (1992). An epizootic of Heterorhabditis spp. (Heterorhabditidae: Nematoda) in sugarcane scarabaeids (Coleoptera). Fund Appl Nematol., 15: 71-73.
  4. Alumai, A. and Grewal, P.S. (2004). Tankmix-comaptibility of the entomopathogenic nematodes, Heterorhabditis bacteriophora and Steinnernema carpocapsae, with selected chemical pesticides using in turfgrass. Biocontrol Sci Tech., 14: 725-730.
  5. Bedding, R.A. (1981). Low cost in vitro production of Neoplectana and Heterorhabditis species (Nematoda) for field control of insect pests. Nematologica., 27: 109-114
  6. Bedding, R.A. (1984). Large scale production, storage and transport of the insect-parasitic nematodes Neoaplectana spp. and Heterorhabditis spp. Ann Appl Bio., 104: 117-120.
  7. Bedding, R.A. (1990). Logistics and strategies for introducing entomopathogenic nematodes technology into developing countries. In: Gaugler, R. and Kaya, H.K. (eds) EPN. Biol. Control., CRC Press, Boca Raton, Florida, pp-233- 248.
  8. Blaxter, M.L, DeLey, P. Gary, J.R., Liu, L.X., Scheldeman, P. and Vierstraete, A. 1998. A molecular evolutionary framework for the phylum Nematoda. Nature., 392: 71-75.
  9. Blinova, S.L. and Ivanova, E.S. (1987) Culturing the nematode-bacterial complex of Neoplectana carpocapsaein insects. In: Sonin, M.D. (ed.) Helminthes of insects, Amerind Publishing, New Delhi, pp-22-26.
  10. Boemare, N.E., Akhurst, R.J. and Mourant, R.G. (1993). DNA relatedness between Xenorhabdus spp. (Enterobacteriaceae), symbiotic bacteria of entomopathogenic nematodes, and a proposal to transfer Xenorhabdus luminescens to a new genus, Photorhabdus gen. nov. Int J Sys Bact., 43: 249-255.
  11. Cabanillas, H.E and Raulston, J.R. (1996). Effects of furrow irrigation on the distribution and infectivity of Steinernema riobrave against corn earworm in corn. Fund Appl Nematol., 19: 273-281.
  12. Cabanillas, H.E. and Raulston, J.R. (1995). Impact of Steinernema riobrave (Rhabditida: Steinernematidae) on the Control of Helicoverpa zea (Lepidoptera: Noctuidae) in Corn. J Econ Entomol., 88(1): 58-64.
  13. Choo, H.Y., Kaya, H.K., Huh, H., Lee, D.W., Kim, H.H., Lee, S.M., Choo, Y.M., (2002). Entomopathogenic nematodes (Steinernema spp. and Heterorhabditis bacteriophora) and a fungus Beauveria brongniartii for biological control of the white grubs, Ectinihoplia rufipes and Exomala orientalis, in Korean golf courses. BioControl., 47: 177– 192.
  14. Choo, H.Y., Kaya, H.K., Kim, J.B., Park, Y.D., (1991). Evaluation of entomopathogenic nematodes, Steinernema carpocapsae (Steinernematidae) and Heterorhabditis bacteriophora (Heterorhabditidae) against rice stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae). Korean J Appl Entomol., 30: 50–53.
  15. Choo, H.Y., Kim, H.H., Lee, D.W., Park, Y.D., (1996). Microbial control of fly maggots with entomopathogenic nematodes and fungus in outhouses of farm houses. Korean J Appl Entomol., 35: 80–84.
  16. Choo, H.Y., Lee, S.M., Chung, B.K., Park, Y.D., Kim, H.H., (1995). Pathogenicity of Korean entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) against local agricultural and forest insect pests. Korean J Appl Entomol., 34: 314–320.
  17. Divya, K. Sankar, M. and Marulasiddesha, K.N. (2010). Efficacy of entomopathogenic nematode, Heterorhabditis indica against three lepidopteran insect pests. Asian J Exp Biol Sci., 1 (1): 183-188.
  18. Dutky, S.R., Thompson, J.V. and Cantwell, G.E. (1964). A technique for the mass propagation of the DD-136 nematode. J Insect Pathol., 6: 417-422.
  19. Ehlers, R.U and Peters, A. (1995). Entomopathogenic nematodes in biological control: feasibility, perspectives and possible risks. In: Hokkanen, HMT and Lynch JM (eds.) Biol. Control: Benefits and Risks. Cambridge University Press, UK, pp- 119-136.
  20. Finnegan, M.M., Downes, M.J., Regan, M.O. and Griffen, C.T., (1999). Effect of salt and temperature stress on survival and infectivity of Heterorhabditis spp. Infective juveniles. Nematology., 1:69-78.
  21. Finney, J.R and Walker, C. (1979). Assessment of a field trial using the DD-136 strain of Neoaplectana sp. for the control of Scolytus scolytus. J Invertebr Patho., 33: 239-241.
  22. Fitters, P.F.L, Patel, M.N, Griffin, C.T and Wright, D.J. (1999). Fatty acid composition of Heterorhabditis sp. during storage. Comp Biochem Physiol.,124: 81-88.
  23. Flanders, K.L., Miller, J.M. and Shields, E.J. (1996). In vivo production of Heterorhabditis bacteriophora ‘Oswego’ (Rhabditida: Heterorhabditidae), a potential biological control agent for soil-inhabiting insects in temperate regions. J Econ Entomol., 89:373-380.
  24. Friedman, M.J. (1990). Commercial production and development. In: Gaugler, R. and Kaya, H.K. (eds) Entomopathogenic nematodes in Biological Control. CRC Press, Boca Raton, Florida, 153-172.
  25. Gaugler, R. (1981). Biological control potential of neoaplectanid nematodes. J Nematol, 13: 241-249.
  26. Gaugler, R. (1988). Ecological considerations in the biological control of soil-inhabiting insect pests with entomopathogenic nematodes. Agricult Ecosys Environ., 24:351-360.
  27. Gaugler, R. (1991). Biological control potential of neoaplectanid nematodes. J Nematol, 13: 241-249.
  28. Gaugler, R. and Georgis, R. (1991). Culture method and efficacy of entomopathogenic nematodes (Rhabditida: Steinernematids and Heterorhabditidae). Bio. Control., 1:269-274.
  29. Gaugler, R. and Han, R. (2002). Production technology. In: Gaugler, R. (ed.) Entomopathogenic Nematology. CAB International, Wallingford, UK, 289-310.
  30. Gaugler, R. and Kaya, H.K. (1990). EPN. Bio Control. Boca Raton, FL: CRC Press.
  31. Georgis, R. and Poinar, G.O Jr. (1983). Effect of soil texture on the distribution and infectivity of Neoplectana carpocapsae (Nematoda: Steinernematidae). J Nematol, 15: 308-311.
  32. Georgis, R. and R. Gaugler. (1991). Predictability in biological control using entomopathogenic nematodes. J Econ Entomol., [Forum] 84: 713-20.
  33. Georgis, R., Dunlop, D.B and Grewal, P.S. (1995). Formulation of entomopathogenic nematodes. In FR Hall and JW Barry (eds.), Biorational Pest Control Agents : Formulation and Delivery, ACS Symposium Series No. 595. Amer. Chem. Soc., Washington, DC, pp-197-205.
  34. Georgis, R., H. Kaya, and Gaugler, R. (1991). Effect of steinernematid and heterorhabditid nematodes on nontarget arthropods. Environ Entomol., 20: 815-22.
  35. Georgis, R., Koppenhoper, A.M., Lacey, L.A., Belair, G., Duncan, L.W., Grewal, P.S., Samish, M., Tan, L., Torr, P. and VanTol, R.W.H.M. 2006. Successes and failures in the use of parasitic nematodes for pest control. Biol Control., 38: 103–123.
  36. Glaser, R.W., McCoy, E.E., Girth, H.B. (1940). The biology and economic importance of a nematode parasitic in insects. J Parasitol. 26: 479-495.
  37. Glazer, I. (2002). Survival biology. In: Gaugler R 9ed.) EPN., CAB International, Wallingford, UK, pp-169-187.
  38. Grewal, P. S., R-U, Ehlers, and D. I. Shapiro-Ilan. (2005). Nematodes as Biocontrol Agents. CABI, New York, NY. 
  39. Grewal, P.S. (2002). Formulation and application technology. In: Gaugler R (ed.) EPN. Nematol.,CAB International, Wallingford, UK pp-265-287.
  40. Griffin, C.P., Finnegon, M.M. and Downes, M.J. (1994). Environmental Tolerances and the dispersal of Heterorhabdites: Survival and infectivity of European Heterorhabditis following prolonged immersion in seawater. Fundam and Appl Nematol., 17:415-421.
  41. Han, R., Liu, X., Xu, J., Xie, R., (1996). Effects of entomopathogenic nematodes on the spatial distribution pattern of litchi beetle, Aristobia testudo larvae in litchi orchards and the economic threshold. Natural Enemies of Insects 18, 99–105.
  42. Hazir, S., Kaya, H.K., Stock, S.P., Keskun, N. (2003). Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk J Biol., 27: 181-202.
  43. Head, J.,Walters, K.F.A., (2003). Augmentation biological control utilizing the entomopathogenic nematode, Steinernema feltiae, against the South American leafminer, Liriomyza huidobrensis. Proc. First Int. Symp. Biol. Control, 13– 18 January 2003, Hawaii, USA. USDA Forest Service, FHTET-03-05, pp-136–140.
  44. Hominick, W. M. (2002). Biogeography. In: Gaugler, R. (Ed.), EPN., CABI, New York, NY, pp-115-143. 
  45. Hussaini, S.S, Nagesh, M. and Shakeela, V. (2005). Survival of infective juvenile of entomopathogenic nematodes under storage and their infectivity against Galleria mellonella and Spodoptera litura. Indian J Plant Prot., 33 (1): 68-71.
  46. Hussaini, S.S., (2003). Progress of research work on entomopathogenic nematodes in India. In: Hussaini, S.S., Rabindra, R.J., Nagesh, M. (Eds.), Current status of research on entomopathogenic nematodes in India. PDBC, Bangalore, India, pp. 27–68.
  47. Hussaini, S.S., Singh, S.P., Parthasarathy, R. and Shakeela, V. (2000). Virulence of native entomopathogenic nematodes against black cutworms, Agrotis ipsilon (Hufnagel) and A. segetum (Noctuidae: Lepidoptera). Indian J Nematol., 30(1): 86-110.
  48. Iiboshi, H, Izono, Y., (2003). Official test results of Steinernema carpocapsae for control of red palm weevil (Rhychopherous ferrugineus). In: Report of Novel Pesticide Practical Examination. Japan Corp. Protection Association, Komagome, Tokyo, pp. 228–231. (In Japanese).
  49. Ishibashi, N. (1993). Integrated control of insect pests by Steinernema carpocapsae. In: Bedding RA, Akhurst RJ and Kaya HK (eds.) Nematodes and the Biological Control of Insects. CSIRO Publications, East Melbourne, Australia, pp-105-113.
  50. Jagdale, G.B., Casey,M.L., Grewal, P.S., Lindquist, R.K., (2004). Eûects of application rate and timing, potting medium and host plant on eûcacy of Steinernema feltiae against fungus gnat, Bradysia coprophila, in ûoriculture. Biol Control., 29: 296–305.
  51. Kang, Y.J., Lee, D.W., Choo, H.Y., Lee, S.M., Kweon, T.W., Shin, H.K., 2004. Biological control of Spodoptera depravata (Butler) (Lepidoptera: Noctuidae) using entomopathogenic nematodes. Korean J Appl Entomol., 43, 61–70.
  52. Kaya, H. (2002). Natural enemies and other antagonists. In Gaugler R (ed.). EPN., CAB International, Wallingford, UK, pp-189-203.
  53. Kaya, H.K. (1987). Diseases caused by nematodes. In Epizootiology of Insect Diseases, ed. JR Fuxa, Y tanada, pp. 453- 470. New York: Wiley and Sons.
  54. Kaya, H.K. and Gaugler, R. (1993). Entomopathogenic Nematodes. Annu Rev Entomol., 38: 181-206.
  55. Kaya, H.K. and Stock, P. (1997). Techniques in insect nematology. In: Lacey, L.A. (ed.) Manual of Techniques in Insect pathology. Academic Press, San Diego, California, pp-282-324.
  56. Kaya, H.K., Burlando, T.M. and Thurston, G.S. (1993). Two entomopathogenic nematode species with different search strategies for insect suppression. Environ Entomol., 22: 859-864.
  57. Khatri-Chhetri, H.B., Waeyenberg,e L., Manandhar, H.K. and Moens, M. (2010). Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Nepal. J Invertebr Pathol. 103: 74-78.
  58. Kim, H.H., Choo, H.Y., Kaya, H.K., Lee, D.W., Lee, S.M., Jeon, H.Y., (2004). Steinernema carpocapsae (Rhabditida: Steinernematidae) as a biological control agent against the fungus gnat Bradysia agrestis (Diptera: Sciaridae) in propagation house. Biocontrol Sci Technol., 14: 171–183.
  59. Kim, H.H., Choo, H.Y., Lee, D.W., Lee, S.M., Jeon, H.Y., Cho, M.R., Yiem, M.S., (2003). Control eYcacy of Korean entomopathogenic nematodes against fungus gnat, Bradysia agrestis (Diptera: Sciaridae) and persistence in bed soil. J Korean Soc Hort Sci., 44:393–401.
  60. Kim, H.H., Choo, H.Y., Lee, H.S., Park, C.G., Lee, D.W., Jin, B.R., Choo, Y.M., 2001a. Biological control of Lycoriella mali (Diptera: Sciaridae), a pest of oyster mushroom, Pleurotus ostreatus using entomopathogenic nematodes. Korean J Appl Entomol., 40: 59–67.
  61. Kim, H.H., Choo, H.Y., Park, C.G., Lee, S.M., Choo, Y.M., 2001b. Biological control of cotton caterpillar, Palpita indica Saunder (Lepidoptera: Pyralidae) with entomopathogenic nematodes. Korean J Appl Entomol., 40: 245–252.
  62. Klein, M.G., (1993). Biological control of scarabs with entomopathogenic nematodes. In: Bedding, R., Akhurst, R., Kaya, H. (Eds.), Nematodes and the Biological Control of Insect Pests. CSIRO Press, East Melbourne, Australia, pp-49–58.
  63. Koppenhöfer, A. M. and P. S. Grewal. (2005). Compatibility and interactions with agrochemicals and other biocontrol agents. In: Nematodes as Biocontrol Agents. CABI, New York, NY, pp-363-381. 
  64. Koppenhofer, A.M. and Kaya, H.K. (1996). Coexistence of two steinernematid nematode species (Rhabditida: Steinernematidae) in the presence of two host species. Appl Soil Ecol., 4: 221-230.
  65. Koppenhöfer, A.M. and Kaya, H.K. (2001). Entomopathogenic nematodes and insect pest management. In: Advances in Biopesticide research, Vol. 2 (O. Koul, Ed.), Harwood Academic Publishers, Amsterdam, The Netherlands. Pp-277-305.
  66. Koppenhofer, A.M., Grewal, P.S. and Kaya, H.K. (2000). Synergism of imidacloprid and entomopathogenic nematodes against white grubs: the mechanism. Entomol Exp Appl., 94: 283-293.
  67. Lalramliana, and Yadav, A.K. (2010). Laboratory evaluation of the pathogenicity of three entomopathogenic nematodes against larvae of cabbage butterfly, Pieris brassicae Linnaeus (Lepidoptera: Pieridae). Science Vision 9 (4): 166- 173.
  68. LeBeck, L.M., Gaugler, R., Kaya, H.K., Hara, A.H., Johnson, M.W., (1993). Host stage suitability of the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to the entomopathogenic nematode Steinernema carpocapsae (Rhabditida: Steinernematidae). J Invertebr Pathol., 62:58–63.
  69. Lee, S.M., Lee, D.W., Choo, H.Y., Kim, D.W., Kim, J.B., (1997). Pathogenicity of entomopathogenic nematodes to some agro-forest insect pests. Korean J Soil Zoo., 2:76–82.
  70. Lewis, E. (2002). Behaviooural ecology. In: Gaugler R (ed.) EPN. CAB International, Wallingford, UK, pp-205-223.
  71. Lewis, E.E. and. Grewal, P. S. (2005). Interactions with plant parasitic nematodes. In: Grewal, P.S., Ehlers, R.-U., and Shapiro-Ilan, D.I. (Eds.), Nematodes as Biocontrol Agents. CABI, New York, NY., pp-349-362.
  72. Lewis, E.E., Campbell, J.F. and Gaugler, R. (1998). A conservation approach to using entomopathogenic nematodes in turf and landscape. In: Barbosa P (ed.) Conserv. Biol. Control,. Academic Press, San Diego, California, pp- 235-254.
  73. Mannion, C.M., McLane, W., Klein, M.G., Moyseenko, J., Oliver, J.B., Cowan, D., 2001. Management of early-instar Japanese beetle (Cole- optera: Scarabaeidae) in ûeld-grown nursery crops. J Econ Entomol.,94: 1151–1161.
  74. Mason, J.M. and Wright, D.J. (1997). Potential for the control of Plutella xylostella larvae with entomopathogenic nematodes. J of Invertebr Pathol., 70: 234-242.
  75. Mathasoliya, J.B, Maghodia, A.B. and Vyas, R.V. (2004). Efficacy of Steinernema riobrave against Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae) on Potato. Indian J Nematol., 34(2):177-179.
  76. Mathur, S.B., Srivastava, R.P., Subba Rao, B.R.S., (1966). Records of nematodes parasitising Chilo zonellus Swinhoe stem borer of maize. Indian J Entomol., 28: 414–415.
  77. Narayanan, K. and Gopalakrishnan, C. (1987). Effect of entomopathogenic nematode, Steinernema feltiae (Rhabditida: Steinernematidae) to the pre-pupa, pupa and adult of Spodoptera litura (Noctuidae: Lepidoptera). Indian J Nematol., 17(2): 273-276.
  78. Nyasani, J.O., Kimenju, J.W., Olubayo, F.M. and Wilson, M.J. (2007). Potential of using entomopathogenic nematodes in the management of diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) in Kenya. Afr Crop Sci Conference Proceedings Vol. 8: 1063-1066.
  79. Park, S.H., Yu YS, Park, J.S., Choo HY, Bae S.D., and Nam M.H. (2001). Biological control of tobacco cutworm, Spodoptera litura Fabricius with entomopathogenic nematodes. Biotechnol. and Bioprocess Engr., 6: 139-143.
  80. Piggott, S.J., Wright, D.J., Mathews, G.A., (2000). Polymeric formulation for the application of entomopathogenic nematodes against foliar pests. The BCPC Conference: Pests and diseases, Vol. 3. Proceedings of International Conference, Brighton, UK, 13–16 November 2000.
  81. Poinar, G.O. (1979). Nematodes for biological Control of Insects. CRC Press, Boca Raton. Florida.
  82. Prabhuraj, A., Patil, B.V. and Shivaleela. (2006). Pathogenicity of Heterorhabditis indicus against Helicoverpa armigera (Hubner). Indian J Entomol., 68(1): 36-38.
  83. Qiu, L. and Bedding, R. (2000). Energy metabolism and its relation to survival and infectivity of infective juveniles of Steinernema carpocapsae under aerobic conditions. Nematology., 2: 551-559.
  84. Rao, Y.R.V.J., Prakash Rao, P.S., Verma, A., Israel, P., (1971). Tests with an insect parasitic nematode DD-136 against the rice stem borer, Tryporyza incertulas. Indian J Entomol. 33:215–217.
  85. Raulston, J.R, Pair, S.D., Loera, J. and Cabanilla,s H.E. (1992). Prepupal and pupal parasitism of Helicoverpa zea and Spodoptera frugiperda (Lepidoptera: Noctuidae) by Steinernema sp. In cornfields in the lower Rio Grande valley. J Econ Entomol., 85(5): 1666-1670.
  86. Salem, S.A., Abdel-Rahman, H.A., Zebitz, C.P.W, Saleh, M.M.E,, Ali Fawkia, I. and El-Kholy, M.Y. (2007). Evaluation of entomopathogenic nematodes in controlling some cabbage pests. J Appl Sci Res., 31 (4): 323-328.
  87. Saravanapriya, B. and Subramanian, S. (2007). Pathogenicity of epn to certain foliar insect pests. Ann of Plant Pro Sci., 15(1): 219-222.
  88. Schroede,r W.J. and Beavers, J.B. (1987). Movement of the entomopathogenous nematodes of the families Heterorhabitidae and Steinernematidae in soil. J Nematol,. 19: 257-259.
  89. Schroeder, P.C, Ferguson, C.S., Shelton, A.M., Wilsey, W.T., Hoffmann, M.P. and Petzoldt, C. (1996). Greenhouse and field evaluation of entomopathogenic nematodes (Nematoda: Heterorhabditidae and Steinernematidae) for control of cabbage maggot (Diptera: Anthomyiidae) on cabbage. J Econ Entomol., 89(5): 1109-1115.
  90. Selvan, S., Gaugler, R. and Lewis, E.E. (1993). Biochemical energy reserves of entomopathogenic nematodes. J of Parasitol., 79: 167-172.
  91. Sexton, S.B. and Williams, P. (1981). A natural occurrence of parasitism of Graphagnathus luecoloma (Boheman) by the nematode Heterorhabditis sp. J Austr Entomol Soc., 20: 253-255.
  92. Shapiro-Ilan D. I. and R. Gaugler. (2002). Production technology for entomopathogenic nematodes and their bacterial symbionts. J Ind Microbiol Biot., 28: 137-146.
  93. Shapiro-Ilan, D. I., D. H. Gouge, and A. M. Koppenhöfer. (2002). Factors affecting commercial success: case studies in cotton, turf and citrus. In: Gaugler, R. (Ed.), EPN., CABI, New York, NY, pp-333-356. 
  94. Shapiro-Ilan, D. I., T. E. Cottrell, R. F. Mizell, D. L. Horton, B. Behle, and C. Dunlap. (2010). Efficacy of Steinernema carpocapsae for control of the lesser peachtree borer, Synanthedon pictipes: Improved aboveground suppression with a novel gel application. Bio Control., 54: 23–28.
  95. Shapiro-Ilan, D.I., D. H. Gouge, S. J. Piggott, and J. Patterson Fife. (2006). Application technology and environmental considerations for use of entomopathogenic nematodes in biological control. Bio Control., 38: 124-133. 
  96. Siddiqui, A.U., Pariha,r A. and Sharma, S.R. (2010). Importance and utilization of entom,opathogenic nematodes for the suppression of insect pests. In: Gupta HCL, Siddiqui AV, Parihar A (eds.) Bio Pest Management (Entomopathogenic Nematodes, Microbes and Bioagents) Agrotech Publishing Academy, Udaipur, pp- 62-78.
  97. Singh, S.P., (1993). Effectiveness of an indigenous nematodes against citrus butterfly. J Insect Sci., 6: 107–108.
  98. Smitley, D.R., Warne,r W.R. and Bird, G.W. (1992). Influence of irrigation and Heterorhabditis bacteriophora on plant- parasitic nematodes in turf. J Nematol., 24 (Suppl.): 637-641.
  99. Strauch, O, Niemann, I., Newmann, A., Schmidt, A.J., Peters, A, and Ehlers, R,U. (2000). Storage and formulation of the entomopathogenic nematodes Heterorhabditis indica and H. bacteriophora. BioControl., 54: 483-500.
  100. Stuart, R. J., M. E. Barbercheck, P. S. Grewal, R.A.J. Taylor, and C. W. Hoy. (2006). Population biology of entomopathogenic nematodes: Concepts, issues, and models. Biol Control., 38: 80-102.
  101. Thruston, G.S., Y.N. and Kaya, H.K. (1994). Influence of salinity on survival and infectivity of entomopathogenic nematodes. J Nematol., 26:345-351.
  102. Wang, J., (1993). Control of the peach fruit moth, Carposina niponensis, using entomopathogenic nematodes. In: Bedding, R., Akhurst, R., Kaya, H. (Eds.), Nematodes and the Biological Control of Insect Pests. CSIRO Publications, East Melbourne, Australia, pp. 59–65.
  103. Wang, Y. and Gaugler, R. (1999). Host and penetration site location by entomopathogenic nematodes against Japanese beetle larvae. J Invertebr Pathol., 72: 313-318.
  104. Wardlow, L.R., Piggott, S., Goldsworthy, R., (2001). Foliar application of Steinernema feltiae for the control of ûower thrips. Medeelingen Faculteit Landbouwkundige Toegepaste Biol. Wetenschappen Univ. Gent 66, 285–291.
  105. White, G.F. (1927). A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.
  106. Williams, E.C., MacDonald, O.C., (1995). Critical factors required by the nematode Steinernema feltiae for the control of the leaf miners, Liriomyza huidobrensis, Liriomyza bryoniae and Chromatomyia syngenesiae. Ann Appl Bio. 127: 329–341.
  107. Williams, E.C., Walters, K.F.A., (2000). Foliar application of the entomopathogenic nematode Steinernema feltiae against leaf miners on vegetables. Biocontrol Sci and Tech., 10: 61–70.
  108. Woodring, J.L. and Kaya, H.K. (1988). Steinernematid and Heterorhabditid Nematodes: A Handbook of Biology and Techniques. Southern Cooperative Bulltin 331, Arkansas Agricultural Experiment Station, Fayettville, Arkanas, 30- pp.
  109. Xu, J., Han, R., Liu, X., Cao, L., Yang, P., (1995). Application of codling moth nematode Steinernema carpocapsae against the larvae of litchi longhorn beetle Aristobia testudo. Acta Phytophylacica Sinica 22: 12–16.
  110. Yang, P., Liu, J., Liu, N., Han, R., Zhang, Y., Yu, C., (2000). Field control of the apple fruit moth Carposina nip ponensis by using Steinernema carpocapsae nematodes. Nat Ene Insects., 22: 79–82.

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