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Current IssueEditorial BoardOnline First ArticlesArchive

volume 37 issue 4 (august 2014) : 379-386,   Doi: 10.5958/0976-0571.2014.00647.X

SELECTION OF SUPERIOR PEAT-BASED BRADYRHIZOBIUM INOCULANTS FOR THE CULTIVATION OF INDIGO (Indigofera tinctoria L.)

A
A.K.M. Mosharof Hossain
M
Musharraf Hossain Mian1
M
M. Rafiqul Islam1
M
M.J. Islam2
M
M.M. Hanafi3
A
Abdul Shukor Juraimi4
F
Farzad Aslani4
M
M.A. Hakim2
3
3*
1Department of Soil Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur–5200, Bangladesh.
Cite article:- Hossain Mosharof A.K.M., Mian1 Hossain Musharraf, Islam1 Rafiqul M., Islam2 M.J., Hanafi3 M.M., Juraimi4 Shukor Abdul, Aslani4 Farzad, Hakim2 M.A., 3* (2025). SELECTION OF SUPERIOR PEAT-BASED BRADYRHIZOBIUM INOCULANTS FOR THE CULTIVATION OF INDIGO (Indigofera tinctoria L.). Legume Research. 37(4): 379-386. doi: 10.5958/0976-0571.2014.00647.X.

ABSTRACT

The pot experiment was conducted to the peat-based Bradyrhizobium inoculants as biofertilizer for cultivation of indigo (Indigofera tinctoria L.).  Six isolates of Bradyrhizobium of indigo origin (HSTU-IR2, HSTU-IR3, HSTU-IR4, HSTU-IR9, HSTU-IR10 and HSTU-IR14) were collected from the Department of Soil Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh. Peat-based Bradyrhizobium inoculants were prepared with these six isolates and the inoculants were tested for their effects on nodulation, growth and yield of indigo plants through a pot experiment. The results revealed that the isolate HSTU-IR3 recorded the highest number and weight of nodules, more amount of N content in nodules, highest plant shoot and weight. The isolate HSTU-IR4 and HSTU-IR3 produced the tallest plant, largest shoots and roots N content. Based on the overall results two isolates viz. HSTU-IR3 and HSTU-IR4 performed superior to others.

REFERENCES

  1. AOAC (Association of Official Analytical Chemists). (1980). Official Analysis, 13th Ed., Washington, D.C.
  2. Agustin, E.O., Ortal, C.I., Pascua, S.R., Jr. Sta C.P.C., Padre, A.T., Ventura ,W.B., Obien, S.R. and Ladha, J.K. (1999). Role of Indigo in improving the productivity of rainfed lowland rice-based cropping systems. Special issue. The Rainfed Lowland Rice Research Consortium. Exper. Agric. 35: 201-210.
  3. Bakasso, S., Lamien-Meda, A., Lamien, C.E., Kiendrebeogo, M., Millogo J., Ouedraogo, A.G., Nacoulma, O.G. (2008) Polyphenol contents and antioxidant activities of five Indigofera species (Fabaceae) from Burkina Faso. Pak J Biol Sci 11:1429–1435.
  4. BARC (Bangladesh Agricultural Research Council) (1997). Fertilizer Recommendation Guide-1997. Bangladesh Agril. Res. Council. New Airport, Dhaka-1215: 25.
  5. Barrios, E. and Cobo, G. (2004). Plant growth, biomass production and nutrient accumulation by slash/mulch agroforestry systems in tropical hillsides of Colombia. Agrof. Syst. 60: 255-265.
  6. Basu, P.K. and Kabi, M. C. (1987). Effect of application of biofertilizers on the growth and nodulation of seven forest legumes. Indian-Forester. 113:249-259.
  7. Bhuiya, M.S.A., Kashem, M. A., Hossain, S.M.A. and Talukder, M.S.U. (1995). Effect of green manuring with Sesbania rostrata in presence of urea-N on transplanted aman rice. Bangladesh J. Agril. Sci. 22:677-679.
  8. Dahot, M. U. (1999). Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves. J. Ethnopharmacology. 64:277-282.
  9. Dallwitz, A. (2000).General system for coding taxonomic description. Taxon. 29: 41-164.
  10. Doignon-Bourcier, F., Willems, A., Coopman, R. Laguerre,G. Gillis,M. AND Lajudie, P. D. (2000).Genotypic Characterization of Bradyrhizobium Strains Nodulating Small Senegalese Legumes by 16S-23S rRNA Intergenic Gene Spacers and Amplified Fragment Length Polymorphism Fingerprint Analyses. Applied and environmental microbiology. 5: 3987–3997.
  11. Essa, T.A. and Al-Dulaimi, H. M. (1985). Nodulation response of soybean to nitrogen application and inoculation. Iraqi J. Agric. Sci. 3:27-35.
  12. FAO (Food and Agriculture Organization) (1997). Plant resources approval of Bangladesh for Agricultural Development. Rep. 2. Agro-ecological Regions of Bangladesh. UNDP.FAO. Rome: 116.
  13. Fuentes, J.B., Abe, M., Uchiumi, T., Suzuki, A. and Higashi S. (2002). Symbiotic root nodule bacteria isolated from yam bean (Pachyrhizus erosus). J Gen Appl Microbiol. 48:181–191.
  14. Hoben, H. J. and Sonasegaran, P. (1982).Comparison of pour spread, and drop plate methods for enumeration of rhizobium spp. in inoculants made from presterilized peat. Appl. Environ.Microbiol.44:1246-1247.
  15. Hossain, A.K.M.M., Mian, M.H., Hakim, M. A., Islam, M.M. and Ferdous, J. (2012). Isolation and Selection of Bradyrhizobium from the Root Nodules of Indigo (Indigoffera tinctiria L.). African J. Biotech. 11:12183- 12191.
  16. Kavimani S., Jaykar B., Clercg E.D., Pannecouque, C., Witvrouw, M. And Clercg, E. (2000). Studies of anti-HIV activity of Indigofera tinctoria. Hamdard Medicus. 43:5-7.
  17. Olsen, S. R., Cose, C. V., Watnabe, F. S. and Dean, L. A. (1954). Phosphorus. In: Methods of Soil Analysis, Part-2, Amer. Soc Agron., Inc. Soil Sci. Soc. Amer., Inc. Madison, Wisconsin, USA.
  18. Page, A. L., Miller, R.H. and Keeney, D.R. (1989). Methods of soil analysis, Part-2, Amer. Soc. Agron., Inc. Soil Sci. Soc. Amer., Inc. Madison, Wisconsin, USA. pp. 152-531.
  19. Pongsilp, N. and Boonkerd, N. (2007). Research techniques for estimating phenotypic and genotypic diversity of root- and stem-nodule bacteria. Suranaree J Sci Technol. 14:297– 308.
  20. Rani, B.P. and Kodandaramaiah, D. (1997). Response of soybean (Glycine max L) to inoculation with varying nitrogen levels. Indian J. Agron. 42:135-137.
  21. Somasegaran, P. and Hoben, H. J. (1985). Methods in Legume-Rhizobium technology. NifTAL Project and Mircen. Univ. Hawaii.
  22. Sreepriya, M., Devaki, T., Balakrishna K. and Appranantham T. (2001). Effect of Indigofera tinctoria Linn. on liver antioxidant defense systems during D-galactosamine/endotoxin induced acute hepatitis in rodents. Indian J. Exper. Biol. 39: 181-184.
  23. Singh, R.P., Gupta, M. K., Verendr S. and Singh V. (2001). Studies on root behavior, biomass production and morphological characters of Indigofera gerardiana wall. in Western Himalayas. Indian Forester. 116 (7): 584-588.
  24. Vincent, J.M. (1970). A Manual for the Practical Study of Root Nodule Bacteria. Blackwall Scientific Pub. Ltd.13. Oxford, England.
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    volume 37 issue 4 (august 2014) : 379-386,   Doi: 10.5958/0976-0571.2014.00647.X

    SELECTION OF SUPERIOR PEAT-BASED BRADYRHIZOBIUM INOCULANTS FOR THE CULTIVATION OF INDIGO (Indigofera tinctoria L.)

    A
    A.K.M. Mosharof Hossain
    M
    Musharraf Hossain Mian1
    M
    M. Rafiqul Islam1
    M
    M.J. Islam2
    M
    M.M. Hanafi3
    A
    Abdul Shukor Juraimi4
    F
    Farzad Aslani4
    M
    M.A. Hakim2
    3
    3*
    1Department of Soil Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur–5200, Bangladesh.
    Cite article:- Hossain Mosharof A.K.M., Mian1 Hossain Musharraf, Islam1 Rafiqul M., Islam2 M.J., Hanafi3 M.M., Juraimi4 Shukor Abdul, Aslani4 Farzad, Hakim2 M.A., 3* (2025). SELECTION OF SUPERIOR PEAT-BASED BRADYRHIZOBIUM INOCULANTS FOR THE CULTIVATION OF INDIGO (Indigofera tinctoria L.). Legume Research. 37(4): 379-386. doi: 10.5958/0976-0571.2014.00647.X.

    ABSTRACT

    The pot experiment was conducted to the peat-based Bradyrhizobium inoculants as biofertilizer for cultivation of indigo (Indigofera tinctoria L.).  Six isolates of Bradyrhizobium of indigo origin (HSTU-IR2, HSTU-IR3, HSTU-IR4, HSTU-IR9, HSTU-IR10 and HSTU-IR14) were collected from the Department of Soil Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh. Peat-based Bradyrhizobium inoculants were prepared with these six isolates and the inoculants were tested for their effects on nodulation, growth and yield of indigo plants through a pot experiment. The results revealed that the isolate HSTU-IR3 recorded the highest number and weight of nodules, more amount of N content in nodules, highest plant shoot and weight. The isolate HSTU-IR4 and HSTU-IR3 produced the tallest plant, largest shoots and roots N content. Based on the overall results two isolates viz. HSTU-IR3 and HSTU-IR4 performed superior to others.

    REFERENCES

    1. AOAC (Association of Official Analytical Chemists). (1980). Official Analysis, 13th Ed., Washington, D.C.
    2. Agustin, E.O., Ortal, C.I., Pascua, S.R., Jr. Sta C.P.C., Padre, A.T., Ventura ,W.B., Obien, S.R. and Ladha, J.K. (1999). Role of Indigo in improving the productivity of rainfed lowland rice-based cropping systems. Special issue. The Rainfed Lowland Rice Research Consortium. Exper. Agric. 35: 201-210.
    3. Bakasso, S., Lamien-Meda, A., Lamien, C.E., Kiendrebeogo, M., Millogo J., Ouedraogo, A.G., Nacoulma, O.G. (2008) Polyphenol contents and antioxidant activities of five Indigofera species (Fabaceae) from Burkina Faso. Pak J Biol Sci 11:1429–1435.
    4. BARC (Bangladesh Agricultural Research Council) (1997). Fertilizer Recommendation Guide-1997. Bangladesh Agril. Res. Council. New Airport, Dhaka-1215: 25.
    5. Barrios, E. and Cobo, G. (2004). Plant growth, biomass production and nutrient accumulation by slash/mulch agroforestry systems in tropical hillsides of Colombia. Agrof. Syst. 60: 255-265.
    6. Basu, P.K. and Kabi, M. C. (1987). Effect of application of biofertilizers on the growth and nodulation of seven forest legumes. Indian-Forester. 113:249-259.
    7. Bhuiya, M.S.A., Kashem, M. A., Hossain, S.M.A. and Talukder, M.S.U. (1995). Effect of green manuring with Sesbania rostrata in presence of urea-N on transplanted aman rice. Bangladesh J. Agril. Sci. 22:677-679.
    8. Dahot, M. U. (1999). Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves. J. Ethnopharmacology. 64:277-282.
    9. Dallwitz, A. (2000).General system for coding taxonomic description. Taxon. 29: 41-164.
    10. Doignon-Bourcier, F., Willems, A., Coopman, R. Laguerre,G. Gillis,M. AND Lajudie, P. D. (2000).Genotypic Characterization of Bradyrhizobium Strains Nodulating Small Senegalese Legumes by 16S-23S rRNA Intergenic Gene Spacers and Amplified Fragment Length Polymorphism Fingerprint Analyses. Applied and environmental microbiology. 5: 3987–3997.
    11. Essa, T.A. and Al-Dulaimi, H. M. (1985). Nodulation response of soybean to nitrogen application and inoculation. Iraqi J. Agric. Sci. 3:27-35.
    12. FAO (Food and Agriculture Organization) (1997). Plant resources approval of Bangladesh for Agricultural Development. Rep. 2. Agro-ecological Regions of Bangladesh. UNDP.FAO. Rome: 116.
    13. Fuentes, J.B., Abe, M., Uchiumi, T., Suzuki, A. and Higashi S. (2002). Symbiotic root nodule bacteria isolated from yam bean (Pachyrhizus erosus). J Gen Appl Microbiol. 48:181–191.
    14. Hoben, H. J. and Sonasegaran, P. (1982).Comparison of pour spread, and drop plate methods for enumeration of rhizobium spp. in inoculants made from presterilized peat. Appl. Environ.Microbiol.44:1246-1247.
    15. Hossain, A.K.M.M., Mian, M.H., Hakim, M. A., Islam, M.M. and Ferdous, J. (2012). Isolation and Selection of Bradyrhizobium from the Root Nodules of Indigo (Indigoffera tinctiria L.). African J. Biotech. 11:12183- 12191.
    16. Kavimani S., Jaykar B., Clercg E.D., Pannecouque, C., Witvrouw, M. And Clercg, E. (2000). Studies of anti-HIV activity of Indigofera tinctoria. Hamdard Medicus. 43:5-7.
    17. Olsen, S. R., Cose, C. V., Watnabe, F. S. and Dean, L. A. (1954). Phosphorus. In: Methods of Soil Analysis, Part-2, Amer. Soc Agron., Inc. Soil Sci. Soc. Amer., Inc. Madison, Wisconsin, USA.
    18. Page, A. L., Miller, R.H. and Keeney, D.R. (1989). Methods of soil analysis, Part-2, Amer. Soc. Agron., Inc. Soil Sci. Soc. Amer., Inc. Madison, Wisconsin, USA. pp. 152-531.
    19. Pongsilp, N. and Boonkerd, N. (2007). Research techniques for estimating phenotypic and genotypic diversity of root- and stem-nodule bacteria. Suranaree J Sci Technol. 14:297– 308.
    20. Rani, B.P. and Kodandaramaiah, D. (1997). Response of soybean (Glycine max L) to inoculation with varying nitrogen levels. Indian J. Agron. 42:135-137.
    21. Somasegaran, P. and Hoben, H. J. (1985). Methods in Legume-Rhizobium technology. NifTAL Project and Mircen. Univ. Hawaii.
    22. Sreepriya, M., Devaki, T., Balakrishna K. and Appranantham T. (2001). Effect of Indigofera tinctoria Linn. on liver antioxidant defense systems during D-galactosamine/endotoxin induced acute hepatitis in rodents. Indian J. Exper. Biol. 39: 181-184.
    23. Singh, R.P., Gupta, M. K., Verendr S. and Singh V. (2001). Studies on root behavior, biomass production and morphological characters of Indigofera gerardiana wall. in Western Himalayas. Indian Forester. 116 (7): 584-588.
    24. Vincent, J.M. (1970). A Manual for the Practical Study of Root Nodule Bacteria. Blackwall Scientific Pub. Ltd.13. Oxford, England.
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