Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 6 (december 2017) : 1019-1026

Skp2 expression exhibits a negative correlation with P27Kip1 in lungs of SD rat stress model induced by lipopolysaccharide

Youbao Zhong, Xiaofen Hu, Hui Guo, Yong Li
1<p>College of Animal Science and Technology,&nbsp;Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China</p>
Cite article:- Zhong Youbao, Hu Xiaofen, Guo Hui, Li Yong (2017). Skp2 expression exhibits a negative correlation with P27Kip1 in lungs ofSD rat stress model induced by lipopolysaccharide . Indian Journal of Animal Research. 51(6): 1019-1026. doi: 10.18805/ijar.v0iOF.8469.

ABSTRACT

In order to investigate action mechanism of Skp2 in SD rat stress model continuously challenged with LPS, not only the histopathological changes but also the distribution and expression of Skp2 and P27Kip1 were detected in lungs from SD rats by HE and immunohistochemistry staining respectively. According to present results, Skp2 and P27Kip1 mianly distributed in the epithelia of respiratory bronchioles, pseudostratified columnar ciliated epithelia of bronchus and vascular endothelial, 
a few were located at alveolar wall. As well, area, IOD and IOD/area of Skp2 observably increased in LPS-treated rats (P<0.05 or P<0.01), but IOD and IOD/area of P27Kip1 apparently decreased in LPS-treated rats (P<0.05 or P<0.01). In conclusion, moderate pathological changes existed in lungs from SD rat stress model challenged by LPS. Moreover, Skp2 high level was negatively correlated with P27Kip1 low expression in lungs, it is indicated that Skp2 probably became a key indicator during stress disease process

REFERENCES


  1. Abraham E, Carmody A, Shenkar R and Arcaroli J. (2000). Neutrophils as early immunologic effectors in hemorrhage- or endotoxemia-    induced acute lung injury. Am J Physiol Lung Cell Mol Physiol., 279:L1137-L1145.

  2. Bashir T and Pagano M. (2004). Don’t skip the G1 phase: how APC/CCdh1 keeps SCFSKP2 in check. Cell Cycle, 3:850-852.

  3. Bloom J and Pagano M. (2003). Deregulated degradation of the cdk inhibitor p27 and malignant transformation. Semin Cancer Biol., 13:41-47.

  4. Cao W, Zhang W, Liu J, Wang Y, Peng X, Lu D, Qi R, Wang Y and Wang H. (2011). Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-alpha and IL-1beta release and augmentation of IL-10 production. Int Immunopharmacol., 11:172-178.

  5. Carrano AC, Eytan E, Hershko A and Pagano M. (1999). SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat Cell Biol., 1:193-199.

  6. Cooke PS, Holsberger DR, Cimafranca MA, Meling DD, Beals CM, Nakayama K, Nakayama KI and Kiyokawa H. (2007). The F box protein S phase kinase-associated protein 2 regulates adipose mass and adipocyte number in vivo. Obesity, 15:1400-1408.

  7. Deshaies RJ. (1999). SCF and Cullin/Ring H2-based ubiquitin ligases. Annu Rev Cell Dev Biol., 15:435-467.

  8. Du Q, Wang C, Zhang N, Li G, Zhang M, Li L, Zhang Q and Zhang J. (2014). In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats. BMC Anesthesiol., 14:117.

  9. Edgar BA and Orr-Weaver TL. (2001). Endoreplication cell cycles: more for less. Cell, 105:297-306.

  10. Faffe DS, Seidl VR, Chagas PS, Goncalves DMV, Capelozzi VL, Rocco PR and Zin WA. (2000). Respiratory effects of lipopolysaccharide-    induced inflammatory lung injury in mice. Eur Respir J., 15:85-91.

  11. Fujita M, Kuwano K, Kunitake R, Hagimoto N, Miyazaki H, Kaneko Y, Kawasaki M, Maeyama T and Hara N. (1998). Endothelial cell apoptosis in lipopolysaccharide-induced lung injury in mice. Int Arch Allergy Immunol., 117:202-208.

  12. Gstaiger M, Jordan R, Lim M, Catzavelos C, Mestan J, Slingerland J and Krek W. (2001). Skp2 is oncogenic and overexpressed in human cancers. Proc Natl Acad Sci USA, 98:5043-5048.

  13. Hamed MR, Hassanein NMA, Zaquqe SAM and Mousa AAR. (2015). Impact of certain immunomodulators on LPS-induced hematotoxicity. Medicinal Chemistry Research, 24:3283-3295.

  14. Harper JW. (2001). Protein destruction: adapting roles for Cks proteins. Curr Biol., 11:R431-R435.

  15. Huang XL, Ma HJ, Zhou XH, Fan YM, Xian XH and Cao H. (2010). Effect of exogenous hydrogen sulfide on polymorphonuclear neutrophil accumulation in acute lung injury rat induced by lipopolysaccharides and its mechanism. Chinese Journal of Applied Physiology, 26:477-480.

  16. Matthay MA, Zimmerman GA, Esmon C, Bhattacharya J, Coller B, Doerschuk CM, Floros J, Gimbrone MJ, Hoffman E, Hubmayr RD, Leppert M, Matalon S, Munford R, Parsons P, Slutsky AS, Tracey KJ, Ward P, Gail DB and Harabin AL. (2003). Future research directions in acute lung injury: summary of a National Heart, Lung, and Blood Institute working group. Am J Respir Crit Care Med., 167:1027-1035.

  17. Nakayama K, Nagahama H, Minamishima YA, Matsumoto M, Nakamichi I, Kitagawa K, Shirane M, Tsunematsu R, Tsukiyama T, Ishida N, Kitagawa M, Nakayama KI and Hatakeyama S. (2000). Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication. EMBO J., 19:2069-2081.

  18. Nakayama K, Nagahama H, Minamishima YA, Miyake S, Ishida N, Hatakeyama S, Kitagawa M, Iemura S, Natsume T and Nakayama KI. (2004). Skp2-mediated degradation of p27 regulates progression into mitosis. Dev Cell, 6:661-672.

  19. Polyak K, Lee MH, Erdjument-Bromage H, Koff A, Roberts JM, Tempst P and Massague J. (1994). Cloning of p27Kip1, a cyclin-    dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell, 78:59-66.

  20. Shim EH, Johnson L, Noh HL, Kim YJ, Sun H, Zeiss C and Zhang H. (2003). Expression of the F-box protein SKP2 induces    hyperplasia, dysplasia, and low-grade carcinoma in the mouse prostate. Cancer Res., 63:1583-1588.

  21. Sutterluty H, Chatelain E, Marti A, Wirbelauer C, Senften M, Muller U and Krek W. (1999). p45SKP2 promotes p27Kip1 degradation and induces S phase in quiescent cells. Nat Cell Biol., 1:207-214.

  22. Suzuki S, Ohashi N and Kitagawa M. (2013). Roles of the Skp2/p27 axis in the progression of chronic nephropathy. Cell Mol Life Sci., 70:3277-3287.

  23. The Acute Respiratory Distress Syndrome Network. (2000). Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. The Acute Respiratory Distress Syndrome Network. N Engl J Med., 342:1301-1308.

  24. Tsushima K, King LS, Aggarwal NR, De Gorordo A, D’Alessio FR and Kubo K. (2009). Acute lung injury review. Intern Med., 48:621-630.

  25. Van der Poll T and Opal SM. (2008). Host-pathogen interactions in sepsis. Lancet Infect Dis., 8:32-43.

  26. Velten M, Britt RJ, Heyob KM, Welty SE, Eiberger B, Tipple TE and Rogers LK. (2012). Prenatal inflammation exacerbates hyperoxia-    induced functional and structural changes in adult mice. Am J Physiol Regul Integr Comp Physiol., 303:R279-R290.

  27. Ware LB and Matthay MA. (2000). The acute respiratory distress syndrome. N Engl J Med., 342:1334-1349.

  28. Weissman AM. (1997). Regulating protein degradation by ubiquitination. Immunol Today, 18:189-198.

  29. Wheeler AP and Bernard GR. (2007). Acute lung injury and the acute respiratory distress syndrome: a clinical review. Lancet, 369:1553-1564.

  30. Wirbelauer C, Sutterluty H, Blondel M, Gstaiger M, Peter M, Reymond F and Krek W. (2000). The F-box protein Skp2 is an ubiquitylation target of a Cul1-based core ubiquitin ligase complex: evidence for a role of Cul1 in the suppression of Skp2 expression in quiescent fibroblasts. EMBO J., 19:5362-5375.

  31. Zhang L and Wang C. (2006). F-box protein Skp2: a novel transcriptional target of E2F. Oncogene, 25:2615-2627.

  32. Zhong YB, Guo H, Hu XF and Li Y. (2015). The histological structure and distribution of mast cells of hedgehog esophagus. Chin J vet sci., 35:1539-1542.

     
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