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Analysis of the polymorphism of expressed sequencetag-Simple sequence repeat in quail

Jun Yan Bai*, You Zhi Pang, Yan Xia Qi, Xiao Hui Zhang and Yin Xian Yun
College of Animal Science and Technology,Henan University of Science and Technology, Luoyang 471003, China.
junyanbai@163.com

Page Range:
Article ID:
B-545
Online Published:
5-10-2016
Abstract
Aiming at accelerating the application of molecular markers in the genetic improvement of quails, six EST-SSR markers were successfully developed using a bioinformatics method. Polymorphisms of three quail populations (Chinese yellow, China black and Korean quail) were detected. The results showed that there were 2-6 alleles in six EST-SSR markers. The mean polymorphism information contents of Chinese yellow , China blackand Korean quail were 0.5451, 0.4962 and 0.4937, respectively. The average heterozygosity values were 0.6134, 0.5759 and 0.5613. Among the six EST-SSR markers, three were highly polymorphicand the others were moderately polymorphic. The newly-developed six EST-SSR markers may be used to determine the genetic diversity of quails. The six EST-SSR markers identified were related to carbohydrate metabolism and melanin synthesis, but the specific mechanisms need to be further analyzed.
Keywords
Bioinformatics, Chinese yellow quail, EST-SSR, Genetic diversity.
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