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Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats 

Sadaf Niaz, Rafi Ullah*, Bahrawar Said, Sumaira Shams, Naser M. AbdEl-Salam and Riaz Ullah

Department of Zoology, Abdul Wali Khan University, Mardan (KPK) Pakistan.

afridiriaz@yahoo.com

Page Range:
Article ID:
B-464
Online Published:
18-04-2016
Abstract

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T .gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii.  The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

Keywords
ELISA, LAT, LFCI, Toxoplasma gondii.
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