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Effect of eubiotic administration to broiler’s feed on intestinal morphology and microbiology under Clostridium perfringens challenge

Ahmed A. Al-Sagan1 and Alaeldein M. Abudabos*2

King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia.

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This study was conducted to assess the effect of prebiotics (Technos), probiotics (GalliPro) and their combination on broilers intestinal histomorphology and bacterial cell counts. 240, one day old chicks were randomly assigned into 6 treatments with eight replicates. Chicks of treatment 1 (control group) were fed unsupplemented diet. The chicks of treatment 2 were fed the control starter and finisher diets and were subjected to Clostridium perfringens infection. The chicks of treatments 3 and 4 were treated as those of treatment 2, but supplemented with antibiotic and probiotics, respectively. Chicks of treatment 5 were treated as those of treatment 2 and given a prebiotic (TechnoMos). Chicks of treatment 6 were treated as those of treatment 2, but given a probiotic (GalliPro) along with prebiotic (TechnoMos). The results showed that the birds that were infected by Clostridium perfringens and were given antibiotic, probiotic, prebiotic or symbiotic had no lesions or hemorrhages. It can, therefore, be concluded that these supplements were helpful in reversing the negative effects of the bacterial challenge. Gram negative Bacilli were found to be the same among all groups (P>0.05), which is an indication that the antibiotic, probiotic, prebiotic or symbiotic tested in this trial had no influence on Gram negative bacteria. The positive modulation in intestinal morphology and microbiology as observed in this study supported the concept that gut condition and function can be improved by dietary supplementation other than AGPs. 

Antimicrobial growth promoters (AGPs), Clostridium perfringens, Prebiotic, Probiotic, Symbiotic.
  1. Abudabos, A.M. and Yehia, H.M. (2013). Effect of dietary mannan oligosaccharide from Saccharomyces cerevisiae on live performance of broilers under Clostridium perfringens challenge. Italian Journal of Animal Science.12:e38.
  2. Abudabos, A. M., Alyemni, A. and Al Marshad, B.A. (2013). Bacillus subtilis PB6 based probiotic (CloSTATTM) improves intestinal morphological and microbiological status of broiler chickens under Clostridium Perfringens challenge. Int. J. Agric. Biol. 15: 978-982.
  3. Alfaro, D.M., Silva, A.V.F., Borges, S.A., Maiorka, F.A., Vargas, S. and Santin, E. (2007). Use of Yucca schidigera extract in broiler diets and its effects on performance results obtained with different coccidiosis control methods. J. Appl. Poult. Res. 16: 248-254.
  4. Al-Ghamdi, M. S., Al-Mustafa, Z.H., El-Morsy, F., Al-Faky, A., Haider, I. and Essa, H. (2000). Residues of tetracycline compounds in poultry products in the eastern province of Saudi Arabia. Public Health, 114 :300-304.
  5. AOAC International. (2000). Official Methods of Analysis of AOAC Int. 17th ed. AOAC International, Gaithersburg, MD.
  6. Baurhoo, B., Ferket, P.R. and Zhao, X. (2009). Effects of diets containing different concentrations of mannanoligosaccharide or antibiotics on growth performance, intestinal development, cecal and litter microbial populations, and carcass parameters of broilers. Poult. Sci. 88:2262-2272.
  7. Bedford, M. (2000). Removal of antibiotic growth promoters from poultry diets: implications and strategies to minimize subsequent problems. World’s Poult. Sci. J. 56: 347–365.
  8. Feighner, S.D. and Dashkevicz, M.P. (1987). Subtherapeutic levels of antibiotics in poultry feeds and their effects on weight gain, feed efficiency, and bacterial cholyltaurine hydrolase activity. Appl. Environm. Microbiol. 53: 331–336.
  9. Fernandez, F., Hinton, M. and Van Gils, B. (2002). Dietary mannan oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella enteritidiscolonization. Avian Pathol. 31:49–58.
  10. Ficken, M.D. and Wages, D.P. (1997). Necrotic enteritis. In: H.J. Barnes, C.W. Beard, L.R. McDougald, Y.M. Saif and B.W. Calnek (ed.). Diseases of poultry. Iowa State University Press, Ames, IA, USA, pp 261-264.
  11. Garridol, M.N., Skjervheim, M., Oppegaard, H. and Sørum, H. (2004). Acidified litter benefits the intestinal flora balance of broiler chickens. Appl. Environm. Microbi., 70: 5208–5213.
  12. Ghanbari, M., Rezaei, M., Jami, M. and Nazari, R.M. (2009). Isolation and characterization of Lactobacillus species from intestinal contents of beluga (Huso huso) and Persian sturgeon (Acipenser persicus). Iran. J. Vet. Res. 10: 152-157.
  13. Jack, R.W., Tagg, J.R. and Ray, B. (1995). Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59: 171–200
  14. Kaldhusdal, M., Schneitz, C., Hofshagen, M. and Skjerve, E. (2001). Reduced incidence of Clostridium perfringens-    associated lesions and improved performance in broiler chickens treated with normal intestinal bacteria from adult fowl. Av. Dis. 45: 149–156.
  15. Knapp, S., Warshow, U., Alexander, K.M., Ho, D., Little, A.M., Fowell, A., Alexander, G., Thursz, M., Cramp, M. and Khakoo, S.I. (2011). A Polymorphism in IL28B Distinguishes Exposed, Uninfected Individuals From Spontaneous Resolvers of HCV Infection. Gastroenterology. 141: 320–325.
  16. Mountzouris, K.C., Tsistsikos, P., Kalamara, E., Nitsh, S., Schatzmayr, G. and Fegeros, K. (2007). Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modualting cecal microflora composition and metabolic activities. Poult. Sci. 86: 309–317.
  17. NRC. (1994). Nutrient Requirements of Poultry. 9th rev. ed. Natl. Acad. Press, Washington DC.
  18. Oliveira, M.C., Rodrigues, E.A., Marques, R.H., Gravena, R.A., Guandolini, G.C. and Moraes, V.M.B. (2008). Performance and morphology of intestinal mucosa of broilers fed mannan-oligosaccharides and enzymes. Arquivo Brasileiro de Medicina Veterinária e Zoo. 60: 442–448.
  19. Porter, Jr, R.E. (1998). Bacterial enteritides of poultry. Poult. Sci. 77: 1159–1165.
  20. Salim, H.M., Kang, H.K., Akter, N., Kim, D.W., Kim, J.H., Kim, M.J., Na, J.C., Jong, H.B., Choi, H.C., Suh, O.S. and Kim, W.K. (2013). Supplementation of direc fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poult. Sci. 92:2084-2090. 
  21. SAS Institute Inc., (2003). SAS Users Guide: Statistics. Version 9.1.3. SAS Institute, Cary, NC.
  22. Snel, J., Harmsen, H. J. M., Van der Wielen, P.W.J.J., and Williams, B. A. (2002). Dietary strategies to influence the gastro-    intestinal microflora of young animals, and its potential to improve intestinal health. Pages 37–69 in Nutrition and Health of the Gastrointestinal Tract.
  23. Teo, A.Y.L. and Tan, H.M. (2006). Effect of Bacillus subtilis PB6 (CloSTAT) on broilers infected with a pathogenic strain of Escherichia coli. J. Appl. Poult. Res. 15: 229–235.
  24. Teo, A.Y.L. and Tan, H.M. (2007). Evaluation of the performance and Intestinal gut microflora of broilers fed on corn-soy diets supplemented with Bacillus subtilis PB6 (CloSTAT). J. Appl. Poult. Res. 16: 296–303.
  25. Zikic, D., Peric, L., Uscebrka, G., Stojanovics, S., Milic, D. and Nollet, L. (2008). Effect of prebiotics in broiler breeder and broiler diets on performance and jejunum morphology of broiler chickens. Pages 879-882 in the 1st Mediterranean Summit of WPSA, Book of Proceedings, Porto Carras, Greece.
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