Indian Journal Of Animal Research
Publish
your articles with us

Quick Facts



Payment Options

payment portals

Click here to pay directly

CHARACTERIZATION OF PROMOTER REGION OF CD14 GENE AND ASSOCIATION WITH CLINICAL MASTITIS IN KARAN FRIES CATTLE

A. Sakthivel Selvan, I.D. Gupta*, Archana Verma, Mahesh V. Chaudhari, Shishir Kanungo and S. Rajesh Kumar
Molecular Genetics Lab, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India
idgupta1959@gmail.com

Page Range:
545-547
Article ID:
B-2817
Online Published:
Abstract
Cluster of Differentiation (CD14) protein was identified as a differentiation marker on the surface of monocytes and macrophages and was characterized as a receptor for bacterial endotoxin (LPS). It is regarded as the first described pattern-recognition receptor (PRR) and one of the excellent candidates for mastitis resistance in cattle. Present study was carried out with the objective to characterize and identify genetic polymorphism of promoter region of CD14 gene and its association with clinical mastitis in lactating Karan Fries cattle maintained at NDRI Karnal. Twelve SNPs were found in the complete sequence of 553 base pairs of promoter region of CD14 gene of Karan Fries cows. Cows were also screened using PCR-RFLP with Hpy188I restriction enzyme which revealed three genotypes AA, AB and BB. All three genotypes differed significantly regarding mastitis incidence.
Keywords
CD14, Karan-Fries, Mastitis, RFLP, SNP.
References
  1. Akira, S., Uematsu, S. and Takeuchi, O. (2006). Pathogen recognition and innate immunity. Cell, 124: 783–801.
  2. Ibeagha-Awemu, E. M., Lee, J. W., Ibeagha, A. E. (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils”. BMC Genetics, 9: 50-60.
  3. Janeway, C. A., Jr., and Medzhitov, R. (2002). Innate immune recognition. Annu. Rev. Immunol., 20: 197–216.
  4. Kumar V. (2012). “Characterization of CD14 gene and its association with mastitis in Sahiwal cattle”. M.V.Sc Thesis. National Dairy Research Institute. Karnal. Haryana.
  5. Ogorevc, J., Kunej, T. and Dovè, P. (2008). An integrated map of cattle candidate genes for mastitis: a step forward to new genetic markers. Acta agriculturae Slovenica, 2: 85–91.
  6. Ogorevc, J., Kunej, T., Razpet A. and Dovè, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet., 40: 832-851.
  7. Pal, A., Sharma, A., Bhattacharya, T. K., Chatterjee, P. N, and Chakravarty A. K. (2011). Molecular characterization and SNP detection of CD14 gene of crossbred cattle. SAGE-Hindawi Access to Research, Molecular Biology International, (2011), article ID 507346, 13 pages, doi: 10.4061/2011/507346.
  8. Paape, M. J., Shafer-Weaver, K., Capuco, A. V., Van Oostveldt, K. and Burvenich, C. (2000). Immune surveillance of mammary tissue by phagocytic cells. Adv. Exp. Med. Biol., 480: 259.277.
  9. Sambrook, J. and Russell, D. W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
Global footprints


© 2015 AARC JOURNALS. All Rights Reserved. Powered By AARC JOURNALS