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Comparative evaluation of antibacterial efficacy of plants traditionally used as antidiarrheal against enteropathogenic Escherichia coli

Ashish Srivastava* and D.B. Mondal

Division of Veterinary Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, India

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The study was conducted to investigate the antibacterial efficacy against enteropathogenic E. coli of plants commonly used to treat calf-diarrhoea. Methanolic extracts of six plants (Aegle marmelos, Curcuma longa, Dalbergia sissoo, Mangifera indica, Psidium guajava and Punica granatum) were screened for their antibacterial property against enteropathogenic E. coli by standard disc diffusion method. Minimum inhibitory concentration (MIC) and of the extract exhibiting highest antibacterial activity was estimated by broth dilution method and minimum bactericidal concentration (MBC) was measured by streaking the contents of MIC tubes on nutrient agar plates. Among the six extracts tested, only extracts of Curcuma longa, Psidium guajava and Punica granatum exhibited antibacterial activity against E. coli. Out of these three, Punica granatum extract was found to be most effective with a mean inhibition zone of 14.67±0.577 mm followed by Psidium guajava (9.67±0.577 mm) and Curcuma longa (8.67±0.577 mm), produced by the disc containing 8.00 mg of respective extract. Minimum inhibitory concentration and minimum bactericidal concentration of the Punica granatum extract were estimated to be 02.00 mg/mL and 03.00 mg/mL respectively. These findings suggest that methanolic extracts of Curcuma longa, Psidium guajava and Punica granatum possess antibacterial activity against enteropathogenic E.coli

Antibacterial, Curcuma longa, Diarrhoea, Escherichia coli, Punica granatum, Psidium guajava
  1. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turk, M. (1966). Antibiotic susceptibility testing by a standardized single disc method, Am. J. Clin. Path., 45(4): 493-496.
  2. Constable, P.D. (2004). Antimicrobial use in the treatment of calf diarrhea, J. Vet. Intern. Med., 18(1): 8-17.
  3. Cowan, M.M. (1999). Plant products as antimicrobial agents, Clin. Microbiol. Rev., 12(4): 564 - 582.
  4. Duke, J.A., Mary, J.O., Bogenschutz, G., Judi-Du Cellier and Duke, P. K. (2000). Handbook of Medicinal Herbs, (2nd ed. CRC press).
  5. Eloff, J.N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? J. Ethnopharmacol., 60: 1 - 8.
  6. Gutierrez, R.M., Mitchell, S. and Solis, R.V. (2008). Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology, J. Ethnopharmacol., 117: 1 - 27.
  7. Haggard, D.L. (1985). Bovine enteric colibacillosis, Vet. Clin. North Am. Food Anim. Pract., 1: 495-508.
  8. Holetz, F.B., Pessini, G.L., Sanches, N.R., Cortez, D.A.G., Nakamura, C.V. and Filho, B.P. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases, Mem. Inst. Oswaldo. Cruz., Rio de Janeiro., 97(7): 1027- 1031.
  9. Laloo, D. and Hemalatha, S. (2011). Ethnomedicinal plants used for diarrhea by tribals of Meghalaya, Northeast India, Pharmacogn. Rev., 5(10): 147–154.
  10. Mazumder, R., Bhattacharya, S., Mazumder, A., Pattnaik, A.K., Tiwary, P.M. and Chaudhary, S. (2006). Antidiarrhoeal evaluation of Aegle marmelos (Correa) Linn. root extract, Phytother. Res., 20(1): 82 - 84.
  11. Mero, K.N., Rollin, R.E. and Phillips, R.W. (1985). Malabsorption due to selected oral antibiotics, Vet. Clin. North Am. Food Anim. Pract., 1(3): 581 -588.
  12. Negi, P.S., Jayaprakasha, G.K., Jaganmohan, R.L. and Sakariah, K.K. (1999). Antimicrobial activity of turmeric oil: a by-    product from curcumin manufacture, J. Agric. Food Chem., 47: 4297 - 4300. 
  13. Niamsa, N. and Sittiwet, C. (2009). Antimicrobial activity of Curcuma longa aqueous extract, J. Pharmacol. Toxicol., 4(4): 173-177.
  14. Ozcelik, B., Orhan, I. and Toker, G. (2006). Antiviral and antimicrobial assessment of some selected flavonoids, Z. Naturforsch., 61(9-10): 632 - 638. 
  15. Prashanth, D., Asha, M.K. and Amit, A. (2001). Antibacterial activity of Punica granatum, Fitoterapia., 72 (2): 171 - 173.
  16. Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. (2000). Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 9thed, (W.B. Saunders Company Ltd). pp: 779 - 801.
  17. Rani, P. and Khullar, N. (2004). Antimicrobial evaluation of some medicinal plants for their anti - enteric potential against multi-drug resistant Salmonella typhi, Phytother. Res., 18: 670 - 673.
  18. Scalbert, A. (1991). Antimicrobial properties of tannins, Phytochemistry, 30(12): 3875-3883.
  19. Snedecor, G. W. and Cochran, W. G. (1994). Statistical Methods. 8th ed, Oxford and IBH, New Delhi).
  20. Voravuthikunchai, S., Lortheeranuwat, A., Jeeju, W., Sririrak, T., Phongpaichit, S. and Supawita, T. (2004). Effective medicinal plants against Enterohaemorrhagic Escherichia coli O157:H7, J. Ethnopharmacol., 94(1): 49-54.
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