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volume 48 issue 1 (february 2014) : 11-13,   Doi: 10.5958/j.0976-0555.48.1.003

CD14 GENE POLYMORPHISM USING HINFI RESTRICTION ENZYME AND ITS ASSOCIATION WITH MASTITIS IN SAHIWAL CATTLE

V
Virendra Kumar
I
I.D. Gupta*
A
Archana Verma
S
S. Rajesh Kumar
M
M.V. Chaudhari
1Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal- 132 001, India
Cite article:- Kumar Virendra, Gupta* I.D., Verma Archana, Kumar Rajesh S., Chaudhari M.V. (2025). CD14 GENE POLYMORPHISM USING HINFI RESTRICTION ENZYME AND ITS ASSOCIATION WITH MASTITIS IN SAHIWAL CATTLE. Indian Journal of Animal Research. 48(1): 11-13. doi: 10.5958/j.0976-0555.48.1.003.

ABSTRACT

Cluster of diffferention-14 (CD14) gene is involved in innate immunity and has been identified as one of the candidate gene for disease resistance. CD14 molecule works as cell surface receptor present on surface of inflammatory cells.  Bovine CD14 gene spans over 2630 bp having 2 exons. Present study is based on identification of DNA polymorphism and to analyze the association between CD14 gene variants with incidence of mastitis in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. PCR-RFLP analysis of contig 4 using HinfI restriction enzyme showed three different genotypic patterns (CC, CD and DD). Remaining contig-enzyme combinations showed monomorphic pattern.  Genotypic frequency of CC genotype was found highest (54%). Chi-square (c2) analysis revealed that there is significant difference between these genotypes regarding mastitis incidence. CC genotyped animals were found to be less susceptible for mastitis.

REFERENCES

  1. Beecher, C., Daly, M., Childs, S., Berry, D.P., Magee, D.A., McCarthy, T.V. and Giblin, L. (2010). Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genetics, 11: 99-108.
  2. Beutler, B. and Rietschel, E.T. (2003). Innate immune sensing and its roots: the story of endotoxin. Nat. Rev. Immunol., 3 (2): 169–176.
  3. Bishop, S.C. and Mackenzie, K.M. 2003. Genetic management strategies for controlling infectious diseases in livestock populations. Genet. Sel. Evol., 35 (1): S3-S17.
  4. Ibeagha-Awemu, E.M., Lee, J.W. and Ibeagha, A.E. (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils. BMC Genetics, 9(50), doi: 10.1186/1471-2156-9-50.
  5. Ogorevc, J., Kunej, T., Razpet, A. and Dovc, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim.Genet, 40: 832–851.
  6. Sambrook, J. and Russell, D.W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
  7. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. 8th edn., Iowa State Univ. Press, Ames, Iowa.
  8. Ulevitch, R.J. and Tobias, P.S. (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 13: 437-457.
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Indian Journal of Animal Research
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    volume 48 issue 1 (february 2014) : 11-13,   Doi: 10.5958/j.0976-0555.48.1.003

    CD14 GENE POLYMORPHISM USING HINFI RESTRICTION ENZYME AND ITS ASSOCIATION WITH MASTITIS IN SAHIWAL CATTLE

    V
    Virendra Kumar
    I
    I.D. Gupta*
    A
    Archana Verma
    S
    S. Rajesh Kumar
    M
    M.V. Chaudhari
    1Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal- 132 001, India
    Cite article:- Kumar Virendra, Gupta* I.D., Verma Archana, Kumar Rajesh S., Chaudhari M.V. (2025). CD14 GENE POLYMORPHISM USING HINFI RESTRICTION ENZYME AND ITS ASSOCIATION WITH MASTITIS IN SAHIWAL CATTLE. Indian Journal of Animal Research. 48(1): 11-13. doi: 10.5958/j.0976-0555.48.1.003.

    ABSTRACT

    Cluster of diffferention-14 (CD14) gene is involved in innate immunity and has been identified as one of the candidate gene for disease resistance. CD14 molecule works as cell surface receptor present on surface of inflammatory cells.  Bovine CD14 gene spans over 2630 bp having 2 exons. Present study is based on identification of DNA polymorphism and to analyze the association between CD14 gene variants with incidence of mastitis in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. PCR-RFLP analysis of contig 4 using HinfI restriction enzyme showed three different genotypic patterns (CC, CD and DD). Remaining contig-enzyme combinations showed monomorphic pattern.  Genotypic frequency of CC genotype was found highest (54%). Chi-square (c2) analysis revealed that there is significant difference between these genotypes regarding mastitis incidence. CC genotyped animals were found to be less susceptible for mastitis.

    REFERENCES

    1. Beecher, C., Daly, M., Childs, S., Berry, D.P., Magee, D.A., McCarthy, T.V. and Giblin, L. (2010). Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genetics, 11: 99-108.
    2. Beutler, B. and Rietschel, E.T. (2003). Innate immune sensing and its roots: the story of endotoxin. Nat. Rev. Immunol., 3 (2): 169–176.
    3. Bishop, S.C. and Mackenzie, K.M. 2003. Genetic management strategies for controlling infectious diseases in livestock populations. Genet. Sel. Evol., 35 (1): S3-S17.
    4. Ibeagha-Awemu, E.M., Lee, J.W. and Ibeagha, A.E. (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils. BMC Genetics, 9(50), doi: 10.1186/1471-2156-9-50.
    5. Ogorevc, J., Kunej, T., Razpet, A. and Dovc, P. (2009). Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim.Genet, 40: 832–851.
    6. Sambrook, J. and Russell, D.W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
    7. Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. 8th edn., Iowa State Univ. Press, Ames, Iowa.
    8. Ulevitch, R.J. and Tobias, P.S. (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol., 13: 437-457.
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