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Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam

M. Hussain*, P. Chakravarty, S. Soren, S. Deori and S. S. Begum
National Research Centre on Yak, Dirang, West Kameng - 790 101, India.

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The study was undertaken to see the effect of recovery techniques and culture media on in-vitro maturation (IVM) of oocytes indigenous cattle of Assam. Oocytes were collected by aspiration and dissection techniques and were graded as A, B, C and D on the basis of their cumulus cell layers surrounding the zona pellucida. Oocytes with A, B and C categories were subjected to IVM using three different media (TCM-199, Ham’s F-12 and Ham’s F-10) with same additives (Sodium pyruvate, Gentamicin, L-glutamine, Porcine FSH, Follicular Fluid and HIOCS). Percentage of oocytes with expansion of cumulus cells was found to be the highest in dissection technique using TCM-199  media in comparison to Ham’s F-12 and Ham’s F-10 using same additives. However, it did not differ significantly between techniques. Incidence of Metaphase-I + Metaphase-II was the highest in oocytes recovered by dissection technique and cultured in TCM-199 with additives when incubated at 38.5°C for 24 hours and it differed significantly  between media but there was no significant difference between the recovery techniques. In conclusion, the culture medium TCM-199 along with additives may give better IVM rate of the oocytes recovered by dissection technique from indigenous cattle ovaries of Assam.
Cattle oocytes, Culture media, In-vitro maturation, Recovery technique.
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