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COMPUTER ASSISTED SPERM ANALYSIS OF FRESH AND FROZEN-THAWED HF X KANKREJ (F1) BULLS SEMEN AND THEIR INTERRELATIONSHIP

J.b. Patel1 and A.j. Dhami*
College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand-388 001, India
ajdhami@aau.in

DOI:
Page Range:
315-320
Article ID:
ARCC147
Online Published:
Abstract
A total of 30 good quality semen ejaculates from 5 mature bulls of HF x Kankrej (F1) breed were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The ejaculates immediately after collection in AV were assessed by CASA for fresh sperm motility and velocity parameters. A part of each ejaculate cryopreserved at -196°C in LN2 using standard Tris-citric acid-fructose-yolk-glycerol extender was thawed and assessed for post-thaw analysis of motility and velocity traits on CASA using via-dent stain.  The mean values of motile and progressively motile spermatozoa observed in fresh semen (74.73±1.71 and 51.57±2.61 %) were highly significantly (Ptozoa were significantly and positively interrelated. Thus, CASA appeared to be the fast, accurate and precise means of qualitative evaluation of the fresh and frozen-thawed bull semen.
Keywords
CASA, Crossbred bull, Fresh semen, Frozen-thawed semen, Motility, Velocity.
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