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Miao Long1,2, Jing Li3, Xia Qin2, Yiming Li1, Peng Li1, Guo-Wen Liu2* and Xiaobing Li2*
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 110866, P. R. China

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The objective of this study was to determine the effects of six yeast (Saccharomyces cerivisiae, Candida tropicalis, Pichia burtonii, Candida utilis, Rhodothece glutinis, Geotrichum candidum) on lactate utilization by the predominant ruminal bacterium Selenomonas ruminantium(S. ruminantium). Four different concentration of these yeasts (6g/l, 8g/l, 10g/l, 12g/l) and non-yeast control group were set. The pH and the concentration of lactate, acetate, and propionate in the culture fluids were detected at 12h after adding these different yeasts. The results indicated that lactate uptake can be stimulated by all the six yeast at all of six concentration. When the added yeast concentration was 10g/l-12g/l, lactate uptake by S. ruminantium was achieved the greatest promote. The six yeasts also increased the production of acetate and propionate and the ratio of propionate to acetate. Among the six yeasts, Candida tropicalis, Geotrichum candidum and Candida utilis affecting on above indexes were better than Saccharomyces cervisiae, Pichia burtonii and Rhodothece glutinis.
Lactate utilization, Rumen fermentation, S. ruminantium, Yeast.
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