Indian Journal Of Agricultural Research
your articles with us

Quick Facts

Payment Options

payment portals

Click here to pay directly


Ashok Kumar*, L.D. Bist and S.K. Maurya
Department of Horticulture, College of Agriculture G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, India

Page Range:
Article ID:
Online Published:
To accentuate the field study, biochemical characterization of total twenty one genotypes of low chill Pyrus spp. including relatives were subjected to leaf protein analysis through SDS-PAGE (Sodium dodecyl sulphate polyacrylamide gel electrophoresis). The leaf protein fragments exhibited appreciable polymorphism amongst the twenty one genotypes used for the study. The maximum numbers of protein bands were resolved in Pant Pear-1, Pant Pear-2, Le Conte and Thumb Pear (25 each), while the minimum number were reported in Pant Pear-13 and Pant Pear-18 (13 each), out of total twenty five protein fragments. The zone B comprised of eight bands with their corresponding molecular weight proteins ranging from above 66 kDa to 29 kDa and the major differences in the protein banding pattern are mainly confined to this zone. The protein bands B7 and C5 were found to be present in all the genotypes except Mehal and Pant Pear-9 respectively. Out of twenty five protein bands, ten fragments viz; four fragments (A1, A2, A4 and A5) of zone A, one fragment (B2) of zone B and five fragments (C4, C6, C7, C8 and C10) of zone C were found to be present homogeneously in all the twenty one genotypes. On the basis of UPGMA analysis, twenty one genotypes were categorized into three groups. Cluster one comprised of 3 genotypes namely Pant Pear-18 and Pant Pear-15 and one independent genotypes Pant Pear-13, cluster second comprised of two independent genotypes namely Pant Pear-9 and Mehal, while rest of the genotypes were present in third cluster.
Pear, Pyrus spp., SDS-PAGE, Cluster analysis.
  1. Ghafoor, A and Arshad M. (2008). Seed protein profiling of pisum sativum l., germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity. Pak. J. Bot., 40 : 2315-2321
  2. Gogorcena, Y. and Ortiz, J.M. (1993). Use of multivariate analysis in the taxonomy of Citrus aurantium L. and relatives. 1993. Sci. Hort., 53 : 301-310.
  3. Ladizinsky, G. and Hymowitz, T. (1979). Seed protein electrophoresis in taxonomic and evolutionary studies. Theor. Appl. Genet., 54 : 145-151.
  4. Leammli, U.K. (1970). Cleavage of structural proteins during the assembly head of bacteriophage T4. Nature. 227: 380-385.
  5. Liao, X.R.; Zhu, X.C. and He, P.C. (1997). Application of seed protein components in cluster analysis of Chinese Vitis plants. J. Hort. Sci., 72 : 109-115.
  6. Mandal, A.B.; Maiti, A.; Chowdhury, B. and Elanchezhian, R. (2001). Isozyme markers in varietal identification of banana. In vitro Cell Dev. Biol. Plant, 37 : 599-604.
  7. Moreno-Arribas, M.V.; Cabello, F.; Polo, M.C.; Martin-Alvarez, P.J. and Pueyo, E. (1999). Assessment of the native electrophoretic analysis of total grape must proteins for the characterization of Vitis vinifera L. cultivar. J. Agric. Food Chem., 47 : 114-120.
  8. Rao, S.Y.; Subbaroy, M.V. and Manga, V. (1992). Electrophoretic analysis of plant genetic variability. Indian Rev. Life Sci., 12 : 147-180.
  9. Rodríguez, G.R., L. Sequin ,G.R. Pratta , R. Zorzoli and L.A. Picardi (2008) Protein profiling in F and F generations of two tomato genotypes differing in ripening time. Biologia Plantarum 52 : 548-552
  10. Smith, J.S.C. and Smith, O.S. 1992. Fingerprinting crop varieties. Adv. Agron. 47: 85-140.
Global footprints

© 2015 AARC JOURNALS. All Rights Reserved. Powered By AARC JOURNALS