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EFFECT OF METAL IONS ON THE CYTOSOLIC NADP+- DEPENDENT ISOCITRATE DEHYDROGENASE FROM GERMINATING BLACKGRAM, VIGNA MUNGO (L.) HEPPER

M. Ayub Ali*, W. Joykumar Singh, L. Inaotombi Devi, S. Kunjeshwori Devi and L. Rupachandra Singh
Department of Biochemistry, Manipur University, Canchipur - 795 003, Manipur

DOI:
Page Range:
267 - 271
Article ID:
ARCC2783
Online Published:
Abstract
The cytosolic NADP+-dependent isocitrate dehydrogenase from germinating blackgram shows
an absolute requirement of a divalent metal ion such as Mn2+ or Mg2+ for expression of its full
activity. Other metal ions like Co2+, Zn2+ and Cu2+ could also activate the enzyme, though the
degree of activation was lower than that of either Mn2+ or Mg2+ at the same concentration. In
contrast, three metal ions Sn2+, Ba2+ and Ca2+ were found to be inhibitory. The kinetics analysis with Mn2+ shows sigmoidal response suggestive of a positive co-operativity in metal ion binding. The A0.5 was 3.3 μM as deduced from a Hill plot with a Hill coefficient value of 2.1.
Keywords
References
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