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Genetic diversity in Cinnamomum tamala Nees. accessions throughDNA fingerprinting using molecular markers

Garima Gwari, Ujjwal Bhandari, Gaurav Naik, S. Zafar Haider* and Nirpendra Chauhan
Centre for Aromatic Plants (CAP),Industrial Estate Selaqui, Dehradun-248 011, India.
zafarhaider.1@rediffmail.com

Page Range:
446-450
Article ID:
A-4360
Online Published:
17-08-2016
Abstract
The present study aims to investigate the genetic variation in Cinnamomum tamala Nees. (Lauraceae) accessions collected from different locations of Uttarakhand Himalaya. The leaves samples were analyzed by using RAPD and ISSR markers. A total 22 primers were used for initial screening in order to select the ones giving good amplification. Seven primers (OPA-12, OPA-18, OPB-10, OPB-17, ISSR-21, ISSR-24 and ISSR-30) were found to be polymorphic in eight accessions. Based on combined profile of ISSR and RAPD markers the dendrogram was constructed by using UPGMA cluster analysis and all the accessions showed discrimination from one another. All the accessions were clustered into two major groups, one containing T1-T4 and second T5-T8. The percentage of polymorphic bands was 86.3 % for three ISSR markers and 70.5 % for four RAPD markers. This emphasize that ISSR markers were found to be the best for genetic variation in the species.
Keywords
Cinnamomum tamala, Genetic diversity, ISSR, Polymorphic, RAPD.
References
  1. Anonymous. (1992). Wealth of India: Raw Materials, Vol II, Publication and Information Directorate, CSIR: New Delhi, India.
  2. Baruah, A.., Nath, S.C. and Hazarika, A.K. (2007). Investigation of the essential oil of Cinnamomum tamala Nees. grown at Jorhat, Assam. Indian Perfumer, 5 : 50-52.
  3. Chauhan, N.K. (2014). Agro technologies and post harvest management in aromatic crops. Indian Perfumer, 58: 49-60. 
  4. Chauhan, N.K., Haider, S.Z., Lohani, H., Sah, S. and Yadav, R.K. (2009). Quality evaluation of Cinnamomum tamala Nees. collected from different locations of Uttarakhand. J. Non-Timber Forest Prods., 16: 191-94.
  5. Chaurasia, J.K., Pandey, N. and Tripathi, Y.B. (2010). Effect of hexane fraction of leaves of Cinnamomum tamala Linn on macrophage functions. Inflammopharmacology, 18: 147–154.
  6. Devarumath, R., Nandy, S., Rani, V., Marimuthu, S., Muraleedharan, N. and Raina, S. (2002). RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp. assamica (Assam-India type). Plant cell report, 21: 166-173.
  7. Devi, S.L, Kannappan, S. and Anuradha, C.V. (2007). Evaluation of in vitro antioxidant activity of Indian bay leaf, Cinnamomum tamala (Buch. -Ham.) T. Nees & Eberm using rat brain synaptosomes as model system. Indian J. Exp. Biol., 45: 778-84.
  8. Dhulasavant, V., Shinde, S., Pawar, M. and Naikwade, N.S. (2010). Antihyperlipidemic activity of Cinnamomum tamala Nees, on high cholesterol diet induced hyperlipidemia. Int J. Pharm. Tech. Res., 2: 2517–2521.
  9. Dighe, V.V., Gursale, A.A., Sane, R.T., Menon, S. and Patel, P.H. (2005). Quantitative determination of eugenol from Cinnamomum tamala Nees and Ebern. Leaf powder and polyherbal formulation using reverse phase chromatography. Chromatographia, 61: 443-446. 
  10. Dongre, A., Parkhi, V. and Gahukar, S. (2004). Characterization of cotton (Gossypium hirsutum) germplasm by ISSR, RAPD markers and agronomic values. Indian J. of Biotechnol., 3: 388-393.
  11. Esselman, E. J., Jianqiang, L., Crawford, D.L., Windus, J.L. and Wolfe, A.D. (1999). Clonal diversity in the rare Calamagrostis porteri spp. Insperata (Poaceae): Comparative result for allozymes and random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Mol. Ecol., 8: 443–451.
  12. Galvan, M Z., Bornet, B., Balatti, P.A. and Branchard, M. (2003). Inter simple sequence repeat (ISSR) marker as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica, 132: 297-301. 
  13. Gambhire, M N., Juvekar, A.R. and Shaijesh, S.W. (2009). Anti-inflammatory activity of aqueous extract of Cinnamomum tamala leaves by in vivo and in vitro methods. J. Pharm Res., 2: 1521-1524.
  14. Hess, J., Kadereit, J.W. and Vargas, P. (2000). The colonization history of Olea europea L. in Macaronesia based on internal transcribed apacer 1 (ITS-1) sequence, randomly amplified polymorphic DNAs (RAPD) and inter-simple sequence repeats (ISSR). Mol. Ecol., 9: 857–868.
  15. Hooker, J.D. (1885). Flora of British India, Bishen Singh Mahendra Pal Singh, Dehradun, 5: 128-136.
  16. Jacob, H.J., Lindpaintner, K., Lincoln, S.E., Kusumi, K., Bunker, R.K., Mao, Y.P., Ganten, D., Dzau, V.J. and Lander, E.S. (1991). Genetic mapping of a gene causing hypertension in the stroke-prone spontaneously hypertensive rats. Cell, 67: 213-224.
  17. Joshi, S., Ranjeka, P. and Gupta, V. (2000). Molecular marker in plants genomes analysis. Curr. Sci, 77: 231-240.
  18. Moreno, S., Martin, J.P. and Ortiz, J.M. (1998). Inter-simple sequence repeats PCR for characterization of closely related grapevine Germplasm. Euphytica, 101: 117-125.
  19. Nagaoka, T. and Ogihara, Y. (1997). Applicability of inter simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Appl. Gen., 94: 597- 602.
  20. Nakamura, Y.M. Lepperl., Connell, P.O., Wolgg, R., Holm, T., Culver, M., Martin, C., Fijimoto, E., Hoff, M., Kumlin, E. and White, R. (1987). Variable number of tandem repeat (VNTR) markers for human gene mapping. Science, 235: 1616–1622.
  21. Nebauer, S.G., Agudo, L.C.D. and Segura, J. (1999). RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.). Theor Appl. Genet, 98: 985-994.
  22. Pandey, A.K., Mishra, A.K. and Mishra, A. (2012). Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomum tamala. Cell Mol Biol (Noisy-le grand), 58: 142-147.
  23. Pharmawati, M., Yan, G. and McFarlane, I.J. (2004). Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae). Aust. Syst. Bot., 17: 49-61.
  24. Rao, C.V., Chaurasia, J.K., Pandey, N. and Tripathi, Y.B. (2010). Effect of hexane fraction of leaves of Cinnamomum tamala Linn on macrophage functions. Inflammopharmacology, 18: 147–154. 
  25. Saghai M.M.A., Soliman, K.M., Jorgensen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the Nat. Acad. Sci. U.S.A. 81: 8014-8018.
  26. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular cloning a laboratory manual, 18-47.
  27. Shradha, B. and Sisodia, S.S. (2011). Assessment of antidiabetic potential of Cinnamomum tamala leaves extract in Streptozotocin induced diabetic rats. Indian J. Pharmacol, 43: 582-585. 
  28. Thimmappaiah, S.W.G., Shobha, D. and Melwyn, G.S. (2006). Assessment of genetic diversity in cashew germplasm using RAPD and ISSR markers. Scientia Hortic., 120: 411-417.
  29. Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res., 18: 7213-7218.
  30. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acid Res., 18: 205-212. 
  31. Yang, W., A.C. Oliveira., I. Godwin., K. Schertz. and J.L. Bennetzen. 1996. Comparison of DNA marker technologies in characterizing plant genome diversity: Variability in Chinese sorghums. Crop. Sci., 36: 1669-1676.
  32. Zietkiewicz, E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by Simple Sequence Repeat (SSR)-anchored polymerase chain reaction amplification, Genomics, 20: 176-183.
     
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