Submit

Asian Journal of Dairy and Food Research, volume 33 issue 4 (december 2014) : 276-284

OPTIMIZATION OF VISCOZYME-L ASSISTED EXTRACTION OF COCONUT MILK AND VIRGIN COCONUT OIL

Ravindra Kumar Agarwal*, S. John Don Bosco
1Department of Food Science and Technology, Pondicherry University, Puducherry, 605 014, India
Cite article:- Agarwal* Kumar Ravindra, Bosco Don John S. (2024). OPTIMIZATION OF VISCOZYME-L ASSISTED EXTRACTION OF COCONUT MILK AND VIRGIN COCONUT OIL. Asian Journal of Dairy and Food Research. 33(4): 276-284. doi: 10.5958/0976-0563.2014.00617.4.

ABSTRACT

The present work aimed to optimize the extraction of milk and oil from coconut by an aqueous enzymatic extraction process using the enzyme Viscozyme-L. The combine effect of grinding time (2-4min), amount of Viscozyme-L (60-180FBGU) and incubation time (2-7h), were optimized using Response Surface Methodology. The selected method provides the best yield of coconut milk (73.88±0.5%, w/w) and Virgin Coconut Oil (21.57±0.4%, w/w) with percentage recovery of oil was 86.14% of fat present in the fresh coconut kernel at grinding time (3min), the amount of enzyme (120FBGU) and incubation time (4.5h). The experimental results were significantly (P

REFERENCES

  1. Agarwal, R. K., and Bosco, S. J. D. (2013). Optimization of aqueous extraction of Virgin Coconut Oil using Response Surface Methodology. Cord (APCC). 29(1): 1-11.
  2. Aliakbarian, B., Faveri, D. De, Converti, A., and Perego, P. (2008). Optimisation of olive oil extraction by means of enzyme processing aids using response surface methodology. Bichem. Eng. J. 42: 34-40.
  3. AOAC (1997). Official Methods of Analysis. 16th edn. Association of Official Analytical Chemists. Washington, D.C.: 367-384.
  4. Asian and Pacific Coconut Community (APCC). Internet: Standard for Virgin Coconut Oil (2009). http://www.apccsec.org/ standards.htm/
  5. Bezerra, M. A., Santelli, R. E., Oliveira, E. P., Villar, L. S., and Escaleira, L. A. (2008). Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta. 76(5): 965-77.
  6. Campbell, K. A., and Glatz, C. E. (2009). Mechanisms of aqueous extraction of soybean oil. J. Agri. Food Chem. 57(22): 10904-12.
  7. Cochran, W. G., and Cox, G. M. (1992). Some methods for the study of response surfaces. In Experimental designs, pp. 335-375. New York: Wiley.
  8. Che Man, Y. B., Asbi, A. B., Azudin, M. N., and Wei, L. S. (1996). Aqueous enzymatic extraction of coconut oil. J. Am. Oil Chem. Soc. 73(6): 683-686.
  9. Danso-Baoteng, E. (2011). Effect of enzyme and heat pretreatment on sunflower oil recovery using Aqueous and Hexane Extractions. Eng. Technol. 80: 839-845.
  10. Evon, P., Vandenbossche, V., Pontalier, P. Y., and Rigal, L. (2009). Aqueous extraction of residual oil from the sunflower press cake using a twin-screw extruder: Feasibility study. Ind.Crop Prod. 29: 455-465.
  11. Firatligil-Durmus, E., and Evranuz, O. (2010). Response surface methodology for protein extraction, optimization of red pepper seed (Capsicum frutescens). LWT - Food Sci. Technol. 43(2): 226-231.
  12. Hellner, G., Sz³yk, E., and Szyd³owska-czerniak, A. (2010). Effect of enzymatic and hydrothermal treatments of rapeseeds on quality of the pressed rapeseed oils: part II. Oil yield and oxidative stability. Process Biochem. 45: 247-258.
  13. Jiang, L., Hua, D., Wang, Z., and Xu, S. (2010). Aqueous enzymatic extraction of peanut oil and protein hydrolysates. Food Bioprod. Process. 88: 233-238.
  14. Krasaechol, N., Chinnasarn, S., Itthisoponkul, T., and Yuenyongputtakal, W. (2011). Use of Viscozyme-L for pre- treatment of coconut prior to extraction by screw press. Thai J. Agr. Sci. 44(5): 436-442. Latif, S., and Anwar, F. (2009). Effect of Aqueous Enzymatic Processes on Sunflower Oil Quality. J. Am. Oil Chem. Soc. 86(4): 393-400.
  15. Latif, S., and Anwar, F. (2011). Aqueous enzymatic sesame oil and protein extraction. Food Chem. 125(2): 679-684.
  16. Lee, W. C., and Yusof, S. (2006). Optimizing conditions for hot water extraction of banana juice using response surface methodology. J. Food Eng.75: 473-479.
  17. Liu, S., Yang, F., Zhang, C., Ji, H., Hong, P., and Deng, C. (2009). Optimization of process parameters for supercritical carbon dioxide extraction of Passiflora seed oil by response surface methodology. The J. Supercrit. Fluids. 48(1): 9-14.
  18. Meziane, S. (2013). Optimization of oil extraction from olive pomace using response surface methodology. Food Sci. Technol. Int. 19(4): 315-322.
  19. Moreau, R. A., Johnston, D. B., Powell, M. J., and Hicks, K. B. (2004). A comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ. J. Am. Oil Chem. Soc. 81(11): 1071-1075.
  20. Moura, J. M. L. N., Campbell, K., Mahfuz, A., Jung, S., Glatz, C. E., and Johnson, L. (2008). Enzyme assisted aqueous extraction of oil and protein from soybeans and cream de-emulsification. J. Am. Oil Chem. Soc. 85(10): 985-995.
  21. Mistakes, G. C. (1980). Recovery of oil from soybeans. In D.R. Erickson (Ed.), Handbook of Soy Oil Processing and Utilization, (pp. 49-65). American Soybean Association and American Oil Chemists’ Society, St. Louis.
  22. Myers, R. H., and Montgomery, D. C. (2002). Response surface methodology: process and product optimization using design experiment (2nd edn.). New York: Wiley.
  23. Nevin, K. G., and Rajamohan, T. (2006). Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food Chem. 99(2): 260-266.
  24. Nevin, K. G., and Rajamohan, T. (2009). Wet and dry extraction of coconut oil: impact on lipid metabolic and antioxidant status in cholesterol co-administered rats. Can. J. Physiol. Pharm. 87(8): 610-616.
  25. Niu, Y. X., Li, W., Zhu, J., Huang, Q., Jiang, M., and Huang, F. (2012). Aqueous enzymatic extraction of rapeseed oil and protein from dehulled cold-pressed double-low rapeseed Cake. Int. J. Food Eng. 8(3): 1-14.
  26. Raghavendra, S. N., and Raghavarao, K. S. M. S. (2010). Aqueous extraction and enzymatic destabilization of coconut milk emulsions. J. Am. Oil Chem. Soc. 88(4): 481-487.
  27. Rustom, I. Y. S., Lopez-Leiva, M. H. and Nair. B. M. (1991). A study of factors affecting extraction of peanut (Arachis hypogaea L.) solids with water. Food Chem. 42: 153-165.
  28. Sant’Anna, B. P. M., Freitas, S. P., and Coelho, M. a. Z. (2003). Enzymatic aqueous technology for simultaneous coconut protein and oil extraction. Grasas Aceites. 54(1): 77-80.
  29. Servili, M., Selvaggini, R., Taticchi, A., Esposto, S., and Montedoro, G. (2003). Volatile compounds and phenolic composition of virgin olive oil: optimization of temperature and time of exposure of olive pastes to air contact during the mechanical extraction process. J. Agr. Food Chem. 51(27): 7980-7988.
  30. Sharma, A., Khare, S. K., and Gupta, M. N. (2002). Enzyme assisted aqueous extraction of peanut oil. J. Am. Oil Chem. Soc. 79(3): 8-11.
  31. Song, Y., Du, B., Zhou, T., Han, B., Yu, F., Yang, Li, Q. (2011). Optimization of extraction process by response surface methodology and preliminary structural analysis of polysaccharides from defatted peanut (Arachis hypogaea) cakes. Carbohyd. Res. 346(2): 305-10.
  32. Tano-Debrah, K., and Ohta, Y. (1997). Aqueous extraction of coconut oil by an enzyme-assisted process. J. Sci. Agr. 74: 497-502.
  33. Womeni, H. M., Ndjouenkeu, R., Kapseu, C., Mbiapo, F. T., Parmentier, M., and Fanni, J. (2008). Aqueous enzymatic oil extraction from Irvingia gabonensis seed kernels. Eur. J. Lipid Sci. Tech. 110(3): 232-238.
  34. Zhang, S. B., Wang, Z., and Xu, S. Y. (2007). Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J. Am. Oil Chem. Soc. 84(1): 97-105.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)