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BACTERICIDAL EFFECT OF MICROWAVE ON ISOLATED BACTERIAL CELLS IN MILK, PANEER AND KHOA

H. Manjunatha*, R. Prabha, B. Ramachandra., R. Krishna and P.A. Shankar
Department of Dairy Microbiology, Dairy Science College, KVAFSU, Hebbal, Bangalore-560 024, India
manjupdk007@rediffmail.com

DOI:
Page Range:
85 - 90
Article ID:
ARCC695
Online Published:
Abstract
The effect of microwave exposure of different menstra inoculated with selected bacterial cultures was investigated. The minimum exposure period in MYG broth required to bring out destruction of Salmonella sp., P.aeruginosa and S.aureus except E.coli, on MYGA and B.subtilis  was 30 sec  with low log count of 0.36 to 0.66. In skim milk the time taken for complete destruction of bacterial cultures when exposed to microwave was 40 sec except E.coli and B.subtilis. B.subtilis showed destruction at 50 sec exposure itself revealing the sensitivity of the culture to microwave. The bacterial cultures inoculated to whole milk at 3.56 to 7.26 and exposed to microwave showed complete destruction at 40 sec exposure except in case of E.coli. The minimum exposure period required to bring out complete destruction of inoculated E.coli, Salmonella sp., and S.aureus except P.aeruginosa and B.subtilis in paneer at 3.54 to 7.08 log count was 50 sec when plated on to selective media while low count of 0.63 to 1.60 noticed on MYGA at the same exposure time. In khoa, the time taken for complete destruction of inoculated E.coli, Salmonella and S.aureus with log count 6.48 to 6.90, when exposed to microwave was 40 sec on specific selective media  but on MYGA the complete destruction noticed by these bacterial cultures was at 50 sec.
Keywords
Microwave, Modified yeast extract broth, Menstra.
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