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AN EFFICIENT PROTOCOL ON PLANT REGENERATION OF UPLAND INDICA RICE (ORYZA SATIVA VARS. GHANTESHWARI AND SIDDHANTA)

Sushil P. Shirsat* and G.R. Rout
Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar- 751 003, India
grrout@rediffmail.com

Page Range:
211-214
Article ID:
ARCC311
Online Published:
Abstract
An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two varieties of indica rice i.e. Ghanteshwari and Siddhanta. Shoot apical meristems from 7-d-old seedlings produced axillary and adventitious shoots on MS media containing 3.0 mg/l 6-benzyladenine (BA). Both the genotypes responded positively on shoot multiplication depending upon media composition. The maximum rate of shoot multiplication was 90%, and the average number of multiple shoots per culture was 12.5 in case of var. Ghanteswari and 10.6 in case of Siddhanta within 4 weeks of culture on 3.0 mg /l BA in combination with 50 mg/l adenine sulphate (Ads). Actively multiplying shoot clumps were recovered with high frequency within 4 weeks of cultures. These excised shoots were rooted on MS medium supplemented with 0.5 mg/l NAA and 3% sucrose. Plantlets were success-fully established in soil. This efficient protocol may be utilized for the production of elite clones and also genetic improvement program.
Keywords
Growth regulator, Micropropagation, Multiple shoots, Rice, Rooting.
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